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Protein

UDP-glucose 6-dehydrogenase

Gene

kfiD

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of UDP-glucuronic acid which is required for K5 capsule synthesis.

Catalytic activityi

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei257 – 2571By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 2318NADSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. UDP-glucose 6-dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
  2. UDP-glucuronate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Capsule biogenesis/degradation

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00038; UER00491.
UPA00934.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 6-dehydrogenase (EC:1.1.1.22)
Short name:
UDP-Glc dehydrogenase
Short name:
UDP-GlcDH
Short name:
UDPGDH
Gene namesi
Name:kfiD
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392UDP-glucose 6-dehydrogenasePRO_0000074045Add
BLAST

Post-translational modificationi

Phosphorylated on a tyrosine residue. It results in a significant increase of the dehydrogenase activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ47329.

Structurei

3D structure databases

ProteinModelPortaliQ47329.
SMRiQ47329. Positions 5-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028357. UDPglc_DH_bac.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500134. UDPglc_DH_bac. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47329-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGTLKITVS GAGYVGLSNG ILMAQNHEVV AFDTHQKKVD LLNDKLSPIE
60 70 80 90 100
DKEIENYLST KILNFRATTN KYEAYKNANY VIIATPTNYD PGSNYFDTSS
110 120 130 140 150
VEAVIRDVTE INPNAIMVVK STVPVGFTKT IKEHLGINNI IFSPEFLREG
160 170 180 190 200
RALYDNLHPS RIIIGECSER AERLAVLFQE GAIKQNIPVL FTDSTEAEAI
210 220 230 240 250
KLFSNTYLAM RVAFFNELDS YAESFGLNTR QIIDGVCLDP RIGNYYNNPS
260 270 280 290 300
FGYGGYCLPK DTKQLLANYQ SVPNKLISAI VDANRTRKDF ITNVILKHRP
310 320 330 340 350
QVVGVYRLIM KSGSDNFRDS SILGIIKRIK KKGVKVIIYE PLISGDTFFN
360 370 380 390
SPLERELAIF KGKADIIITN RMSEELNDVV DKVYSRDLFK CD
Length:392
Mass (Da):44,108
Last modified:November 1, 1997 - v1
Checksum:i69E15A27FCD25C79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77617 Genomic DNA. Translation: CAA54708.1.
PIRiS70197.
RefSeqiWP_001305084.1. NZ_JSSD01000054.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77617 Genomic DNA. Translation: CAA54708.1.
PIRiS70197.
RefSeqiWP_001305084.1. NZ_JSSD01000054.1.

3D structure databases

ProteinModelPortaliQ47329.
SMRiQ47329. Positions 5-392.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ47329.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00038; UER00491.
UPA00934.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028357. UDPglc_DH_bac.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500134. UDPglc_DH_bac. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Region 2 of the Escherichia coli K5 capsule gene cluster encoding proteins for the biosynthesis of the K5 polysaccharide."
    Petit C., Rigg G., Pazzani C., Smith A., Sieberth V., Stevens M., Boulnois G., Jann K., Roberts I.S.
    Mol. Microbiol. 17:611-620(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K5.

Entry informationi

Entry nameiUDG5_ECOLX
AccessioniPrimary (citable) accession number: Q47329
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 4, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.