Q47281T1RE_ECOLXType I restriction enzyme EcoEI endonuclease subunitEcoEIR protein3.1.21.3hsdRhsrEscherichia coliBacteriaPseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichiaConservation of motifs within the unusually variable polypeptide sequences of type I restriction and modification enzymes.NUCLEOTIDE SEQUENCE [GENOMIC DNA]A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.NOMENCLATUREThe restriction (R) subunit of a type I restriction enzyme that recognizes 5'-GAGN(7)ATGC-3' and cleaves a random distance away. Subunit R is required for both nuclease and ATPase activities, but not for modification. After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.The type I restriction/modification system is composed of three polypeptides R, M and S; the restriction enzyme has stoichiometry R(2)M(2)S(1) while the methyltransferase is M(2)S(1).Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.Belongs to the HsdR family.ATP-bindingDNA-bindingEndonucleaseHydrolaseNucleaseNucleotide-bindingRestriction systemATPMAGVNKTHLTETDIITKFILPAVKDAGWDVMSQIRQEVKLRDGKIVVRGKLASRIKVKSADIVLYHKPNLPLAVIEAKANKHAISKGMQQGLDYASLLDVPFVFASNGDGFIFHDKTNPQQLESEIALSDFPTPEQLWQKYCAWKGFTQEQLPVISQDYFDDGSGKAPRYYQMQAINRTVDAVSAGKNRILLVMATGTGKTYTAFQIIWRLWKAKNKKRILFLADRNILVDQTKRNDFQPFGNAMTKVTGRTIDPAYEVHLALYQAITGPEEHQKAYKQVAPDFFDLIVIDECHRGSASEDSAWREILEYFGSATQVGLTATPKETEDVSNIDYFGEPVYTYSLKEGIEDGFLAPYKVVRVDIDVDVQGWRPVKGQLDKYGEEIEDRIYNLKDFDRTLVIDERTMLVAQTITDYLKRTNPMDKTIVFCNDIDHADRMRHALVVLNPEQVLKNEKYVMKITGDDDIGKAQLDNFINPKKAYPVIATTSELMTTGVDAQTCKLVVLDQNIQSMTKFKQIIGRGTRINEKHGKLWFTILDFKKATELFADPRFDGLPEKVLVVKPGDISDKDSDFNEQLDAENNADGGDNDASEAREDVADYQVNRDKQAGNGEFHDDDENKVRKFYVNGVDVKVLAKRVQYYDSDGKLVTESFQDYTRKTMLKDSEYASLDSFVRKWQDAPRKQVIIEELEQLGILWDVLAEEVGKDLDPFDLLCHVVYGQPPLTRQERVANVRKRNYFTKYAEPAQQVLNTLLDKYADEGVQEIEDVQVLKLKPFDTLGRPIEIIKTRFGDKKAYEQAVNELENEIYQLPPRSA
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