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Q47281

- T1RE_ECOLX

UniProt

Q47281 - T1RE_ECOLX

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Protein
Type I restriction enzyme EcoEI R protein
Gene
hsdR, hsr
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

The EcoEI enzyme recognizes 5'-GAGN7ATGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification.

Catalytic activityi

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi195 – 2017ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. Type I site-specific deoxyribonuclease activity Source: UniProtKB-EC
  4. helicase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Type I restriction enzyme EcoEI R protein (EC:3.1.21.3)
Short name:
R.EcoEI
Gene namesi
Name:hsdR
Synonyms:hsr
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Type I restriction enzyme EcoEI R protein
PRO_0000077258Add
BLAST

Proteomic databases

PRIDEiQ47281.

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Structurei

3D structure databases

ProteinModelPortaliQ47281.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini181 – 341161Helicase ATP-binding
Add
BLAST
Domaini410 – 561152Helicase C-terminal
Add
BLAST

Sequence similaritiesi

Belongs to the HsdR family.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR013670. EcoEI_R_C_dom.
IPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
[Graphical view]
PfamiPF08463. EcoEI_R_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47281-1 [UniParc]FASTAAdd to Basket

« Hide

MAGVNKTHLT ETDIITKFIL PAVKDAGWDV MSQIRQEVKL RDGKIVVRGK    50
LASRIKVKSA DIVLYHKPNL PLAVIEAKAN KHAISKGMQQ GLDYASLLDV 100
PFVFASNGDG FIFHDKTNPQ QLESEIALSD FPTPEQLWQK YCAWKGFTQE 150
QLPVISQDYF DDGSGKAPRY YQMQAINRTV DAVSAGKNRI LLVMATGTGK 200
TYTAFQIIWR LWKAKNKKRI LFLADRNILV DQTKRNDFQP FGNAMTKVTG 250
RTIDPAYEVH LALYQAITGP EEHQKAYKQV APDFFDLIVI DECHRGSASE 300
DSAWREILEY FGSATQVGLT ATPKETEDVS NIDYFGEPVY TYSLKEGIED 350
GFLAPYKVVR VDIDVDVQGW RPVKGQLDKY GEEIEDRIYN LKDFDRTLVI 400
DERTMLVAQT ITDYLKRTNP MDKTIVFCND IDHADRMRHA LVVLNPEQVL 450
KNEKYVMKIT GDDDIGKAQL DNFINPKKAY PVIATTSELM TTGVDAQTCK 500
LVVLDQNIQS MTKFKQIIGR GTRINEKHGK LWFTILDFKK ATELFADPRF 550
DGLPEKVLVV KPGDISDKDS DFNEQLDAEN NADGGDNDAS EAREDVADYQ 600
VNRDKQAGNG EFHDDDENKV RKFYVNGVDV KVLAKRVQYY DSDGKLVTES 650
FQDYTRKTML KDSEYASLDS FVRKWQDAPR KQVIIEELEQ LGILWDVLAE 700
EVGKDLDPFD LLCHVVYGQP PLTRQERVAN VRKRNYFTKY AEPAQQVLNT 750
LLDKYADEGV QEIEDVQVLK LKPFDTLGRP IEIIKTRFGD KKAYEQAVNE 800
LENEIYQLPP RSA 813
Length:813
Mass (Da):92,627
Last modified:November 1, 1996 - v1
Checksum:iA1EB1794123F0ADD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L18759 Genomic DNA. Translation: AAD15048.1.
PIRiI41292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L18759 Genomic DNA. Translation: AAD15048.1 .
PIRi I41292.

3D structure databases

ProteinModelPortali Q47281.
ModBasei Search...

Proteomic databases

PRIDEi Q47281.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR013670. EcoEI_R_C_dom.
IPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
[Graphical view ]
Pfami PF08463. EcoEI_R_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Conservation of motifs within the unusually variable polypeptide sequences of type I restriction and modification enzymes."
    Murray N.E., Daniel A.S., Cowan G.M., Sharp P.M.
    Mol. Microbiol. 9:133-143(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A58.

Entry informationi

Entry nameiT1RE_ECOLX
AccessioniPrimary (citable) accession number: Q47281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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