Flavohemoprotein - Dickeya dadantii (strain 3937)

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Q47266 (HMP_DICD3) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 79. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Flavohemoprotein

Alternative name(s):
Flavohemoglobin
Hemoglobin-like protein
Hemoprotein X
Nitric oxide dioxygenase
Short name=NO oxygenase
Short name=NOD
EC=1.14.12.17

Gene names

Name:	hmp
Synonyms:	hmpX
Ordered Locus Names:	Dda3937_03368

Organism

Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) [Complete proteome] [HAMAP]

Taxonomic identifier

198628 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O₂ and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress By similarity. HAMAP MF_01252
Catalytic activity	2 NO + 2 O₂ + NAD(P)H = 2 NO₃^- + NAD(P)⁺. HAMAP MF_01252
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 1 FAD per subunit By similarity. HAMAP MF_01252
Subcellular location	Cytoplasm HAMAP MF_01252.
Domain	Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H. HAMAP MF_01252
Sequence similarities	Belongs to the globin family. Two-domain flavohemoproteins subfamily. In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Detoxification Oxygen transport Transport
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein Heme Iron Metal-binding NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to nitrosative stress Inferred from electronic annotation. Source: InterPro response to toxin Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro nitric oxide dioxygenase activity Inferred from electronic annotation. Source: EC oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 395

395

Flavohemoprotein HAMAP MF_01252

PRO_0000052434

Regions

Domain

150 – 255

106

FAD-binding FR-type

Nucleotide binding

204 – 207

FAD By similarity

Nucleotide binding

268 – 273

NADP By similarity

Nucleotide binding

388 – 391

FAD By similarity

Region

1 – 138

138

Globin HAMAP MF_01252

Region

147 – 395

249

Reductase HAMAP MF_01252

Region

259 – 395

137

NAD or NADP-binding HAMAP MF_01252

Sites

Active site

Charge relay system By similarity

Active site

135

Charge relay system By similarity

Metal binding

Iron (heme proximal ligand) By similarity

Binding site

188

FAD By similarity

Site

Involved in heme-bound ligand stabilization and O-O bond activation By similarity

Site

Influences the redox potential of the prosthetic heme and FAD groups By similarity

Site

387

Influences the redox potential of the prosthetic heme and FAD groups By similarity

Experimental info

Sequence conflict

123

A → R in CAA53500. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q47266 [UniParc].

Last modified November 30, 2010. Version 2.
Checksum: 025225B7587745E6
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FASTA

395

44,230

        10         20         30         40         50         60 
MLDQQTIATI KSTIPLLAET GPALTAHFYQ RMFHHNPELK DIFNMSNQRN GDQREALFNA 

        70         80         90        100        110        120 
ICAYATHIEN LPALLPAVER IAQKHASFNI QPEQYQIVGT HLLATLEEMF QPGQAVLDAW 

       130        140        150        160        170        180 
GKAYGVLANV FIQRESDIYQ QSAGQNGGWH GIRPFRIVAK QPQSSLITSF TLEPVDGGPI 

       190        200        210        220        230        240 
AAFRPGQYLA VYIRDKRFEY QEIRQYSLTN EPNGRYYRIA VKRETMGSVS GYLHDVAREG 

       250        260        270        280        290        300 
DVIELAAPHG DFYLEVTPET PVALISAGVG QTPMLSMLHS LKNQQHQADI FWLHAAENTE 

       310        320        330        340        350        360 
VHAFADEIAD VAATLPQLQS YVWYREASSE AARSAHAFHG LMALKDLPTP LPMTNLHCYL 

       370        380        390 
CGPVAFMQFA ARQLLELGIT ESQIHYECFG PHKVI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Flavohaemoglobin HmpX: a new pathogenicity determinant in Erwinia chrysanthemi strain 3937." Favey S., Labesse G., Vouille V., Boccara M. Microbiology 141:863-871(1995) [PubMed: 7773389] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 3937.
[2]	"Complete genome sequence of Dickeya dadantii 3937." International Erwinia Consortium Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3937.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75893 Genomic DNA. Translation: CAA53500.1. CP002038 Genomic DNA. Translation: ADM99548.1.
PIR	S44277.
RefSeq	YP_003884105.1. NC_014500.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9734799.
GenomeReviews	Gene locus Dda3937_03368 in contig CP002038_GR.
KEGG	ddd:Dda3937_03368.
PATRIC	42318985. VBIDicDad25310_3305.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01252. Hmp. [Tree]
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR009050. Globin-like. IPR012292. Globin_dom. IPR000971. Globin_subset. IPR023950. Hmp. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR001221. Phe_hydroxylase. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.490.10. Globin_related. 1 hit.
KO	K05916.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00042. Globin. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00371. FPNCR. PR00410. PHEHYDRXLASE.
SUPFAM	SSF46458. Globin_like. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. PS01033. GLOBIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMP_DICD3

Accession

Primary (citable) accession number: Q47266
Secondary accession number(s): E0SAY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 30, 2010
Last modified:	January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Family and domain databases

Entry information

Relevant documents