Q471D2 (KATG1_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 1
Short name=CP 1
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	Reut_A1635

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	751 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 751	751	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354876
Sites
	Active site	104	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	100	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	103 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-103) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q471D2 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 927BEE598B274E08	FASTA	751	83,059
	10 20 30 40 50 60 MTDKQHTRSV SESENPAIPS PTPKVSRPRR NKDWWPNQLD LSVLHTHSHL SCPLEEEFDY 70 80 90 100 110 120 ADQFKGLDVD ALKQDLIQLM TTSQDWWPAD YGHYGPLFIR MSWHAAGTYR IADGRGGGGE 130 140 150 160 170 180 GQQRFAPLNS WPDNANLDKA RRLLWPLKKK YGQKVSWADL LIFAGNVAYE SMGFKTFGFG 190 200 210 220 230 240 FGRPDVWEPE DIFWGPEDTW LGDERYSGDR ELAKPLANVQ MGLIYVNPEG PGGNPDPLAA 250 260 270 280 290 300 ARDIRETFAR MAMNDEETVA LIVGGHTVGK THGAAPAAGN VGLEPEGAPI EEQGLGWKNK 310 320 330 340 350 360 FGSGKGSDAI TSGLEGAWTN NPTKWDNGFL ENLFKYEWEL TTSPAGAKQW KPKNPEANDT 370 380 390 400 410 420 VPDAHGASRR HSPTMLTTDL SLRMDPIYGP IAKRFHDNPD QLEDAFAKAW FKLLHRDMGP 430 440 450 460 470 480 RSRYLGPWIP EAQLWQDPIP PVDHELVSEQ DIDALKRTIL GSGLSVPELI STAWGAAASF 490 500 510 520 530 540 RGTDKRGGAN GARIRLAPQK DWESNEPSRL AKVLTALERI QNDFNGSQSG GKKVSLADLI 550 560 570 580 590 600 VLGGCAAVEE AARKAGFNIS VPFAPGRTDA SQEQTDESIF DVLEPIGDAF RNYFRAEDPL 610 620 630 640 650 660 SPETRLLDRA NLLKLTAPQM TVLVGGMRML DANHGQSRHG VFTDKPGTLS NEFFVNLLDI 670 680 690 700 710 720 GTAWRPSVAD KSVYEGIDRT SGKTRWTATA ADLVFGAHSQ LRALAEVYAC DDGKERFVRD 730 740 750 FVAAWNKVMN LDRYDLLGTR NGRRAVTSKP V

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ61001.1.
RefSeq	YP_295845.1. NC_007347.1.
3D structure databases
HSSP	HSSP built from PDB template 2CCA based on UniProtKB Q50555.
ProteinModelPortal	Q471D2.
SMR	Q471D2. Positions 31-736.
ModBase	Search...
Protein-protein interaction databases
STRING	Q471D2.
Protein family/group databases
PeroxiBase	2661. ReCP02_JMP134.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3610060.
GenomeReviews	Gene locus Reut_A1635 in contig CP000090_GR.
KEGG	reu:Reut_A1635.
NMPDR	fig|264198.3.peg.2155.
PATRIC	20228793. VBIRalEut24049_2259.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	QGKFVED.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_A1635-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_CUPPJ

Accession

Primary (citable) accession number: Q471D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program