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Q47163

- T1MP_ECOLX

UniProt

Q47163 - T1MP_ECOLX

Protein

Type I restriction enzyme EcoprrI M protein

Gene

hsdM

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. The EcoprrI enzyme recognizes 5'-CCAN7ATGC-3'.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei254 – 2541S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3552. M.EcoprrI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type I restriction enzyme EcoprrI M protein (EC:2.1.1.72)
    Short name:
    M.EcoprrI
    Gene namesi
    Name:hsdM
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 520520Type I restriction enzyme EcoprrI M proteinPRO_0000088024Add
    BLAST

    Proteomic databases

    PRIDEiQ47163.

    Interactioni

    Subunit structurei

    The type I restriction/modification system is composed of three polypeptides R, M and S.

    Structurei

    3D structure databases

    ProteinModelPortaliQ47163.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni198 – 2036S-adenosyl-L-methionine bindingBy similarity
    Regioni230 – 2323S-adenosyl-L-methionine bindingBy similarity

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR022749. D12N6_MeTrfase_N.
    IPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR004546. Restrct_endonuc_typeI_HsdM.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF12161. HsdM_N. 1 hit.
    PF02384. N6_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00497. hsdM. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q47163-1 [UniParc]FASTAAdd to Basket

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    MKMTSIQQRA ELHRQIWQIA NDVRGSVDGW DFKQYVLGAL FYRFISENFS    50
    SYIEAGDDSI CYAKLDDSVI TDDIKDDAIK TKGYFIYPSQ LFCNVAAKAN 100
    TNDRLNADLN SIFVAIESSA YGYPSEADIK GLFADFDTTS NRLGNTVKDK 150
    NARLAAVLKG VEGLKLGDFN EHQIDLFGDA YEFLISNYAA NAGKSGGEFF 200
    TPQHVSKLIA QLAMHGQTHV NKIYDPAAGS GSLLLQAKKQ FDDHIIEEGF 250
    FGQEINHTTY NLARMNMFLH NINYDKFDIK LGNTLTEPHF RDEKPFDAIV 300
    SNPPYSVKWI GSDDPTLIND ERFAPAGVLA PKSKADFAFV LHALNYLSAK 350
    GRAAIVCFPG IFYRGGAEQK IRQYLVDNNY VETVISLAPN LFFGTTIAVN 400
    ILVLSKHKTD TKVQFIDASE LFKKETNNNI LTDAHIEQIM QVFASKEDVA 450
    HLAKSVAFET VVANDYNLSV SSYVEAKDTR EIIDIAELNA ELKTTVSKID 500
    QLRKDIDAIV AEIEGCEVQK 520
    Length:520
    Mass (Da):58,015
    Last modified:November 1, 1996 - v1
    Checksum:i79401EC1720EB257
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75452 Genomic DNA. Translation: CAA53205.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75452 Genomic DNA. Translation: CAA53205.1 .

    3D structure databases

    ProteinModelPortali Q47163.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3552. M.EcoprrI.

    Proteomic databases

    PRIDEi Q47163.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR022749. D12N6_MeTrfase_N.
    IPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR004546. Restrct_endonuc_typeI_HsdM.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF12161. HsdM_N. 1 hit.
    PF02384. N6_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00497. hsdM. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Escherichia coli prr region encodes a functional type IC DNA restriction system closely integrated with an anticodon nuclease gene."
      Tyndall C., Meister J., Bickle T.A.
      J. Mol. Biol. 237:266-274(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CTR5X.

    Entry informationi

    Entry nameiT1MP_ECOLX
    AccessioniPrimary (citable) accession number: Q47163
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg2+ as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3