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Protein

Type I restriction enzyme EcoprrI M protein

Gene

hsdM

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. The EcoprrI enzyme recognizes 5'-CCAN7ATGC-3'.

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg2+ as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei254S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3552 M.EcoprrI

Names & Taxonomyi

Protein namesi
Recommended name:
Type I restriction enzyme EcoprrI M protein (EC:2.1.1.72)
Short name:
M.EcoprrI
Gene namesi
Name:hsdM
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880241 – 520Type I restriction enzyme EcoprrI M proteinAdd BLAST520

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Structurei

3D structure databases

ProteinModelPortaliQ47163
SMRiQ47163
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni198 – 203S-adenosyl-L-methionine bindingBy similarity6
Regioni230 – 232S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di1.20.1260.30, 1 hit
InterProiView protein in InterPro
IPR022749 D12N6_MeTrfase_N
IPR038333 D12N6_MeTrfase_N_sf
IPR003356 DNA_methylase_A-5
IPR002052 DNA_methylase_N6_adenine_CS
IPR004546 Restrct_endonuc_typeI_HsdM
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF12161 HsdM_N, 1 hit
PF02384 N6_Mtase, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
TIGRFAMsiTIGR00497 hsdM, 1 hit
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q47163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMTSIQQRA ELHRQIWQIA NDVRGSVDGW DFKQYVLGAL FYRFISENFS
60 70 80 90 100
SYIEAGDDSI CYAKLDDSVI TDDIKDDAIK TKGYFIYPSQ LFCNVAAKAN
110 120 130 140 150
TNDRLNADLN SIFVAIESSA YGYPSEADIK GLFADFDTTS NRLGNTVKDK
160 170 180 190 200
NARLAAVLKG VEGLKLGDFN EHQIDLFGDA YEFLISNYAA NAGKSGGEFF
210 220 230 240 250
TPQHVSKLIA QLAMHGQTHV NKIYDPAAGS GSLLLQAKKQ FDDHIIEEGF
260 270 280 290 300
FGQEINHTTY NLARMNMFLH NINYDKFDIK LGNTLTEPHF RDEKPFDAIV
310 320 330 340 350
SNPPYSVKWI GSDDPTLIND ERFAPAGVLA PKSKADFAFV LHALNYLSAK
360 370 380 390 400
GRAAIVCFPG IFYRGGAEQK IRQYLVDNNY VETVISLAPN LFFGTTIAVN
410 420 430 440 450
ILVLSKHKTD TKVQFIDASE LFKKETNNNI LTDAHIEQIM QVFASKEDVA
460 470 480 490 500
HLAKSVAFET VVANDYNLSV SSYVEAKDTR EIIDIAELNA ELKTTVSKID
510 520
QLRKDIDAIV AEIEGCEVQK
Length:520
Mass (Da):58,015
Last modified:November 1, 1996 - v1
Checksum:i79401EC1720EB257
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75452 Genomic DNA Translation: CAA53205.1

Similar proteinsi

Entry informationi

Entry nameiT1MP_ECOLX
AccessioniPrimary (citable) accession number: Q47163
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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