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Protein

mRNA interferase toxin YafO

Gene

yafO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Toxic component of a type II toxin-antitoxin (TA) system. A translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin YafN. Overexpression causes cleavage of a number of mRNAs in a ribosome-dependent fashion. YafO binding to the 50S ribosomal subunit in the translation complex induces mRNA cleavage 3' to the region protected by the ribosome; YafO alone is not able to digest mRNA. mRNA interferases play a role in bacterial persistence to antibiotics; overexpression of this protein induces persisters resistant to ciprofloxacin and ampicillin (PubMed:21788497).3 Publications

GO - Molecular functioni

  • endoribonuclease activity Source: UniProtKB
  • ribosomal large subunit binding Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • negative regulation of translation Source: EcoCyc
  • SOS response Source: UniProtKB-KW

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding
Biological processDNA damage, DNA repair, SOS response, Stress response, Toxin-antitoxin system

Enzyme and pathway databases

BioCyciEcoCyc:G6117-MONOMER
MetaCyc:G6117-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA interferase toxin YafO (EC:3.1.-.-)
Alternative name(s):
Endoribonuclease YafO
Toxin YafO
Gene namesi
Name:yafO
Ordered Locus Names:b0233, JW0223
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13152 yafO

Pathology & Biotechi

Disruption phenotypei

mRNA interferases play a role in bacterial persistence to antibiotics; as 10 mRNA interferases are successively deleted reduced levels of persisters are generated (PubMed:21788497).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001685391 – 132mRNA interferase toxin YafOAdd BLAST132

Proteomic databases

PaxDbiQ47157
PRIDEiQ47157

Expressioni

Inductioni

Induced by amino acid starvation, glucose starvation, the DNA cross-linker mitomycin C (SOS response) and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the YafN antitoxin. Transcription is negatively regulated by the cognate locus, probably by YafN. A member of the dinB-yafNOP operon; it has 2 promoters, 1 upstream of dinB and 1 specific for yafN-yfaO.2 Publications

Interactioni

Subunit structurei

Probably forms a complex with the antitoxin YafN which inhibits the mRNA interferase activity.Curated

Protein-protein interaction databases

BioGridi4259763, 133 interactors
IntActiQ47157, 6 interactors
STRINGi316385.ECDH10B_0215

Structurei

3D structure databases

ProteinModelPortaliQ47157
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG41065DM Bacteria
ENOG410XTZP LUCA
HOGENOMiHOG000117057
KOiK19160
OMAiIAIIKHW

Family and domain databases

InterProiView protein in InterPro
IPR020353 Toxin_YafO
PfamiView protein in Pfam
PF13957 YafO_toxin, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD082093 Uncharacterised_YafO, 1 hit

Sequencei

Sequence statusi: Complete.

Q47157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVFKTKLIR LQLTAEELDA LTADFISYKR DGVLPDIFGR DALYDDSFTW
60 70 80 90 100
PLIKFERVAH IHLANENNPF PPQLRQFSRT NDEAHLVYCQ GAFDEQAWLL
110 120 130
IAILKPEPHK LARDNNQMHK IGKMAEAFRM RF
Length:132
Mass (Da):15,488
Last modified:November 1, 1997 - v1
Checksum:i6EAC0D9E38599CCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA Translation: BAA07595.1
U70214 Genomic DNA Translation: AAB08653.1
U00096 Genomic DNA Translation: AAC73337.1
AP009048 Genomic DNA Translation: BAA77902.1
PIRiB64748
RefSeqiNP_414768.1, NC_000913.3
WP_001263489.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73337; AAC73337; b0233
BAA77902; BAA77902; BAA77902
GeneIDi944916
KEGGiecj:JW0223
eco:b0233
PATRICifig|1411691.4.peg.2050

Similar proteinsi

Entry informationi

Entry nameiYAFO_ECOLI
AccessioniPrimary (citable) accession number: Q47157
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health