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Protein

mRNA interferase YafO

Gene

yafO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. A translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin YafN. Overexpression causes cleavage of a number of mRNAs in a ribosome-dependent fashion. YafO binding to the 50S ribosomal subunit in the translation complex induces mRNA cleavage 3' to the region protected by the ribosome; YafO alone is not able to digest mRNA.2 Publications

GO - Molecular functioni

  • endoribonuclease activity Source: UniProtKB
  • ribosomal large subunit binding Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • negative regulation of translation Source: EcoCyc
  • SOS response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Toxin

Keywords - Biological processi

DNA damage, DNA repair, SOS response, Stress response

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6117-MONOMER.
ECOL316407:JW0223-MONOMER.
MetaCyc:G6117-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA interferase YafO (EC:3.1.-.-)
Alternative name(s):
Endoribonuclease YafO
Toxin YafO
Gene namesi
Name:yafO
Ordered Locus Names:b0233, JW0223
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13152. yafO.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001685391 – 132mRNA interferase YafOAdd BLAST132

Proteomic databases

PaxDbiQ47157.
PRIDEiQ47157.

Expressioni

Inductioni

Induced by amino acid starvation, glucose starvation, the DNA cross-linker mitomycin C (SOS response) and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the YafN antitoxin. Transcription is negatively regulated by the cognate locus, probably by YafN. A member of the dinB-yafNOP operon; it has 2 promoters, 1 upstream of dinB and 1 specific for yafN-yfaO.2 Publications

Interactioni

Subunit structurei

Probably forms a complex with the antitoxin YafN which inhibits the mRNA interferase activity.Curated

Protein-protein interaction databases

BioGridi4259763. 133 interactors.
IntActiQ47157. 6 interactors.
STRINGi511145.b0233.

Structurei

3D structure databases

ProteinModelPortaliQ47157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG41065DM. Bacteria.
ENOG410XTZP. LUCA.
HOGENOMiHOG000117057.
KOiK19160.
OMAiMSIRIFK.

Family and domain databases

InterProiIPR020353. Toxin_YafO.
[Graphical view]
PfamiPF13957. YafO_toxin. 1 hit.
[Graphical view]
ProDomiPD082093. Uncharacterised_YafO. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q47157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVFKTKLIR LQLTAEELDA LTADFISYKR DGVLPDIFGR DALYDDSFTW
60 70 80 90 100
PLIKFERVAH IHLANENNPF PPQLRQFSRT NDEAHLVYCQ GAFDEQAWLL
110 120 130
IAILKPEPHK LARDNNQMHK IGKMAEAFRM RF
Length:132
Mass (Da):15,488
Last modified:November 1, 1997 - v1
Checksum:i6EAC0D9E38599CCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA. Translation: BAA07595.1.
U70214 Genomic DNA. Translation: AAB08653.1.
U00096 Genomic DNA. Translation: AAC73337.1.
AP009048 Genomic DNA. Translation: BAA77902.1.
PIRiB64748.
RefSeqiNP_414768.1. NC_000913.3.
WP_001263489.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73337; AAC73337; b0233.
BAA77902; BAA77902; BAA77902.
GeneIDi944916.
KEGGiecj:JW0223.
eco:b0233.
PATRICi32115581. VBIEscCol129921_0235.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA. Translation: BAA07595.1.
U70214 Genomic DNA. Translation: AAB08653.1.
U00096 Genomic DNA. Translation: AAC73337.1.
AP009048 Genomic DNA. Translation: BAA77902.1.
PIRiB64748.
RefSeqiNP_414768.1. NC_000913.3.
WP_001263489.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ47157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259763. 133 interactors.
IntActiQ47157. 6 interactors.
STRINGi511145.b0233.

Proteomic databases

PaxDbiQ47157.
PRIDEiQ47157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73337; AAC73337; b0233.
BAA77902; BAA77902; BAA77902.
GeneIDi944916.
KEGGiecj:JW0223.
eco:b0233.
PATRICi32115581. VBIEscCol129921_0235.

Organism-specific databases

EchoBASEiEB2946.
EcoGeneiEG13152. yafO.

Phylogenomic databases

eggNOGiENOG41065DM. Bacteria.
ENOG410XTZP. LUCA.
HOGENOMiHOG000117057.
KOiK19160.
OMAiMSIRIFK.

Enzyme and pathway databases

BioCyciEcoCyc:G6117-MONOMER.
ECOL316407:JW0223-MONOMER.
MetaCyc:G6117-MONOMER.

Miscellaneous databases

PROiQ47157.

Family and domain databases

InterProiIPR020353. Toxin_YafO.
[Graphical view]
PfamiPF13957. YafO_toxin. 1 hit.
[Graphical view]
ProDomiPD082093. Uncharacterised_YafO. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiYAFO_ECOLI
AccessioniPrimary (citable) accession number: Q47157
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.