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Protein

Antitoxin YafN

Gene

yafN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin; overexpression prevents YafO-mediated cessation of cell growth and inhibition of cell proliferation.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA repair Source: UniProtKB-KW
  • negative regulation of molecular function Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoCyc
  • SOS response Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

DNA damage, DNA repair, SOS response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6116-MONOMER.
ECOL316407:JW0222-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin YafN
Gene namesi
Name:yafN
Ordered Locus Names:b0232, JW0222
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13151. yafN.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9797Antitoxin YafNPRO_0000168538Add
BLAST

Proteomic databases

PaxDbiQ47156.
PRIDEiQ47156.

Expressioni

Inductioni

Induced by amino acid starvation, glucose starvation, the DNA cross-linker mitomycin C (SOS response) and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the YafN antitoxin. Transcription is negatively regulated by the cognate locus, probably by this protein. A member of the dinB-yafNOP operon; it has 2 promoters, 1 upstream of dinB and 1 specific for yafN-yfaO.2 Publications

Interactioni

Subunit structurei

Probably forms a complex with the mRNA interferase YafO which inhibits the mRNA interferase activity.Curated

Protein-protein interaction databases

BioGridi4259762. 72 interactions.
IntActiQ47156. 2 interactions.
STRINGi511145.b0232.

Structurei

3D structure databases

ProteinModelPortaliQ47156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phD/YefM antitoxin family.Curated

Phylogenomic databases

eggNOGiENOG4105UG9. Bacteria.
COG2161. LUCA.
HOGENOMiHOG000117056.
InParanoidiQ47156.
KOiK19161.
OMAiNNKPAGY.
OrthoDBiEOG6WT8JC.

Family and domain databases

InterProiIPR006442. Antitoxin_Phd/YefM.
[Graphical view]
PfamiPF02604. PhdYeFM_antitox. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRILAEKSV NITELRKNPA KYFIDQPVAV LSNNRPAGYL LSASAFEALM
60 70 80 90
DMLAEQEEKK PIKARFRPSA ARLEEITRRA EQYLNDMTDD DFNDFKE
Length:97
Mass (Da):11,234
Last modified:November 1, 1997 - v1
Checksum:iC49811AE1E90A659
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA. Translation: BAA07594.1.
U70214 Genomic DNA. Translation: AAB08652.1.
U00096 Genomic DNA. Translation: AAC73336.1.
AP009048 Genomic DNA. Translation: BAE76047.1.
PIRiA64748.
RefSeqiNP_414767.1. NC_000913.3.
WP_000554758.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73336; AAC73336; b0232.
BAE76047; BAE76047; BAE76047.
GeneIDi944920.
KEGGiecj:JW0222.
eco:b0232.
PATRICi32115579. VBIEscCol129921_0234.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA. Translation: BAA07594.1.
U70214 Genomic DNA. Translation: AAB08652.1.
U00096 Genomic DNA. Translation: AAC73336.1.
AP009048 Genomic DNA. Translation: BAE76047.1.
PIRiA64748.
RefSeqiNP_414767.1. NC_000913.3.
WP_000554758.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ47156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259762. 72 interactions.
IntActiQ47156. 2 interactions.
STRINGi511145.b0232.

Proteomic databases

PaxDbiQ47156.
PRIDEiQ47156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73336; AAC73336; b0232.
BAE76047; BAE76047; BAE76047.
GeneIDi944920.
KEGGiecj:JW0222.
eco:b0232.
PATRICi32115579. VBIEscCol129921_0234.

Organism-specific databases

EchoBASEiEB2945.
EcoGeneiEG13151. yafN.

Phylogenomic databases

eggNOGiENOG4105UG9. Bacteria.
COG2161. LUCA.
HOGENOMiHOG000117056.
InParanoidiQ47156.
KOiK19161.
OMAiNNKPAGY.
OrthoDBiEOG6WT8JC.

Enzyme and pathway databases

BioCyciEcoCyc:G6116-MONOMER.
ECOL316407:JW0222-MONOMER.

Miscellaneous databases

PROiQ47156.

Family and domain databases

InterProiIPR006442. Antitoxin_Phd/YefM.
[Graphical view]
PfamiPF02604. PhdYeFM_antitox. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "dinP, a new gene in Escherichia coli, whose product shows similarities to UmuC and its homologues."
    Ohmori H., Hatada E., Qiao Y., Tsuji M., Fukuda R.
    Mutat. Res. 347:1-7(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The dinB operon and spontaneous mutation in Escherichia coli."
    McKenzie G.J., Magner D.B., Lee P.L., Rosenberg S.M.
    J. Bacteriol. 185:3972-3977(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUGGESTION OF ANTITOXIN FUNCTION, INDUCTION, OPERON STRUCTURE.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Characterization of YafO, an Escherichia coli toxin."
    Zhang Y., Yamaguchi Y., Inouye M.
    J. Biol. Chem. 284:25522-25531(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN MRNA INTERFERASE ANTITOXIN.
    Strain: K12 / BW25113.
  7. "Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses."
    Christensen-Dalsgaard M., Jorgensen M.G., Gerdes K.
    Mol. Microbiol. 75:333-348(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN MRNA INTERFERASE ANTITOXIN, INDUCTION, OPERON STRUCTURE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiYAFN_ECOLI
AccessioniPrimary (citable) accession number: Q47156
Secondary accession number(s): Q2MCF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 13, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.