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Protein

Antitoxin YafN

Gene

yafN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin; overexpression prevents YafO-mediated cessation of cell growth and inhibition of cell proliferation.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA repair Source: UniProtKB-KW
  • negative regulation of molecular function Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoCyc
  • SOS response Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

DNA damage, DNA repair, SOS response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6116-MONOMER.
ECOL316407:JW0222-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin YafN
Gene namesi
Name:yafN
Ordered Locus Names:b0232, JW0222
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13151. yafN.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001685381 – 97Antitoxin YafNAdd BLAST97

Proteomic databases

PaxDbiQ47156.
PRIDEiQ47156.

Expressioni

Inductioni

Induced by amino acid starvation, glucose starvation, the DNA cross-linker mitomycin C (SOS response) and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the YafN antitoxin. Transcription is negatively regulated by the cognate locus, probably by this protein. A member of the dinB-yafNOP operon; it has 2 promoters, 1 upstream of dinB and 1 specific for yafN-yfaO.2 Publications

Interactioni

Subunit structurei

Probably forms a complex with the mRNA interferase YafO which inhibits the mRNA interferase activity.Curated

Protein-protein interaction databases

BioGridi4259762. 72 interactors.
IntActiQ47156. 2 interactors.
STRINGi511145.b0232.

Structurei

3D structure databases

ProteinModelPortaliQ47156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phD/YefM antitoxin family.Curated

Phylogenomic databases

eggNOGiENOG4105UG9. Bacteria.
COG2161. LUCA.
HOGENOMiHOG000117056.
InParanoidiQ47156.
KOiK19161.
OMAiNNKPAGY.

Family and domain databases

InterProiIPR006442. Antitoxin_Phd/YefM.
[Graphical view]
PfamiPF02604. PhdYeFM_antitox. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRILAEKSV NITELRKNPA KYFIDQPVAV LSNNRPAGYL LSASAFEALM
60 70 80 90
DMLAEQEEKK PIKARFRPSA ARLEEITRRA EQYLNDMTDD DFNDFKE
Length:97
Mass (Da):11,234
Last modified:November 1, 1997 - v1
Checksum:iC49811AE1E90A659
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA. Translation: BAA07594.1.
U70214 Genomic DNA. Translation: AAB08652.1.
U00096 Genomic DNA. Translation: AAC73336.1.
AP009048 Genomic DNA. Translation: BAE76047.1.
PIRiA64748.
RefSeqiNP_414767.1. NC_000913.3.
WP_000554758.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73336; AAC73336; b0232.
BAE76047; BAE76047; BAE76047.
GeneIDi944920.
KEGGiecj:JW0222.
eco:b0232.
PATRICi32115579. VBIEscCol129921_0234.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA. Translation: BAA07594.1.
U70214 Genomic DNA. Translation: AAB08652.1.
U00096 Genomic DNA. Translation: AAC73336.1.
AP009048 Genomic DNA. Translation: BAE76047.1.
PIRiA64748.
RefSeqiNP_414767.1. NC_000913.3.
WP_000554758.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ47156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259762. 72 interactors.
IntActiQ47156. 2 interactors.
STRINGi511145.b0232.

Proteomic databases

PaxDbiQ47156.
PRIDEiQ47156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73336; AAC73336; b0232.
BAE76047; BAE76047; BAE76047.
GeneIDi944920.
KEGGiecj:JW0222.
eco:b0232.
PATRICi32115579. VBIEscCol129921_0234.

Organism-specific databases

EchoBASEiEB2945.
EcoGeneiEG13151. yafN.

Phylogenomic databases

eggNOGiENOG4105UG9. Bacteria.
COG2161. LUCA.
HOGENOMiHOG000117056.
InParanoidiQ47156.
KOiK19161.
OMAiNNKPAGY.

Enzyme and pathway databases

BioCyciEcoCyc:G6116-MONOMER.
ECOL316407:JW0222-MONOMER.

Miscellaneous databases

PROiQ47156.

Family and domain databases

InterProiIPR006442. Antitoxin_Phd/YefM.
[Graphical view]
PfamiPF02604. PhdYeFM_antitox. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYAFN_ECOLI
AccessioniPrimary (citable) accession number: Q47156
Secondary accession number(s): Q2MCF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.