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Protein

Antitoxin YafN

Gene

yafN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Antitoxin component of a type II toxin-antitoxin (TA) system. Functions as an mRNA interferase antitoxin; overexpression prevents YafO-mediated cessation of cell growth and inhibition of cell proliferation.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA repair Source: UniProtKB-KW
  • negative regulation of molecular function Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoCyc
  • SOS response Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processDNA damage, DNA repair, SOS response, Toxin-antitoxin system, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G6116-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin YafN
Gene namesi
Name:yafN
Ordered Locus Names:b0232, JW0222
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13151 yafN

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001685381 – 97Antitoxin YafNAdd BLAST97

Proteomic databases

PaxDbiQ47156
PRIDEiQ47156

Expressioni

Inductioni

Induced by amino acid starvation, glucose starvation, the DNA cross-linker mitomycin C (SOS response) and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the YafN antitoxin. Transcription is negatively regulated by the cognate locus, probably by this protein. A member of the dinB-yafNOP operon; it has 2 promoters, 1 upstream of dinB and 1 specific for yafN-yfaO.2 Publications

Interactioni

Subunit structurei

Probably forms a complex with the mRNA interferase YafO which inhibits the mRNA interferase activity.Curated

Protein-protein interaction databases

BioGridi4259762, 73 interactors
IntActiQ47156, 2 interactors
STRINGi316385.ECDH10B_0214

Structurei

3D structure databases

ProteinModelPortaliQ47156
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phD/YefM antitoxin family.Curated

Phylogenomic databases

eggNOGiENOG4105UG9 Bacteria
COG2161 LUCA
HOGENOMiHOG000117056
InParanoidiQ47156
KOiK19161
OMAiNNKPAGY

Family and domain databases

InterProiView protein in InterPro
IPR006442 Antitoxin_Phd/YefM
IPR036165 YefM-like_sf
PfamiView protein in Pfam
PF02604 PhdYeFM_antitox, 1 hit
SUPFAMiSSF143120 SSF143120, 1 hit

Sequencei

Sequence statusi: Complete.

Q47156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRILAEKSV NITELRKNPA KYFIDQPVAV LSNNRPAGYL LSASAFEALM
60 70 80 90
DMLAEQEEKK PIKARFRPSA ARLEEITRRA EQYLNDMTDD DFNDFKE
Length:97
Mass (Da):11,234
Last modified:November 1, 1997 - v1
Checksum:iC49811AE1E90A659
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA Translation: BAA07594.1
U70214 Genomic DNA Translation: AAB08652.1
U00096 Genomic DNA Translation: AAC73336.1
AP009048 Genomic DNA Translation: BAE76047.1
PIRiA64748
RefSeqiNP_414767.1, NC_000913.3
WP_000554758.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73336; AAC73336; b0232
BAE76047; BAE76047; BAE76047
GeneIDi944920
KEGGiecj:JW0222
eco:b0232
PATRICifig|1411691.4.peg.2051

Similar proteinsi

Entry informationi

Entry nameiYAFN_ECOLI
AccessioniPrimary (citable) accession number: Q47156
Secondary accession number(s): Q2MCF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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