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Protein

REP-associated tyrosine transposase

Gene

rayT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transposase that is always flanked by repeated extragenic palindrome (REP) sequences, which are clustered in structures called bacterial interspersed mosaic elements (BIMEs). RayT catalyzes cleavage and recombination of BIMEs. Binds REP sequences and cleaves BIMEs both upstream and downstream of the REP sequence. Could be important in the creation of BIME variability and amplification.3 Publications

Enzyme regulationi

Cleavage occurs in the presence of magnesium, but is much more pronounced with manganese.1 Publication

GO - Molecular functioni

  • bubble DNA binding Source: EcoCyc
  • DNA binding Source: EcoCyc
  • DNA hairpin binding Source: EcoCyc
  • sequence-specific DNA binding Source: EcoCyc
  • single-stranded DNA binding Source: EcoCyc
  • single-stranded DNA endodeoxyribonuclease activity Source: EcoCyc
  • transposase activity Source: InterPro

GO - Biological processi

  • DNA recombination Source: EcoCyc
  • transposition, DNA-mediated Source: InterPro

Keywordsi

Molecular functionDNA-binding
Biological processTransposition
LigandManganese

Enzyme and pathway databases

BioCyciEcoCyc:G6112-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
REP-associated tyrosine transposase
Gene namesi
Name:rayT1 Publication
Synonyms:TnpAREP1 Publication, yafM
Ordered Locus Names:b0228, JW0218
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13150. rayT.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001685361 – 165REP-associated tyrosine transposaseAdd BLAST165

Proteomic databases

PaxDbiQ47152.
PRIDEiQ47152.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4259773. 31 interactors.
DIPiDIP-11217N.
IntActiQ47152. 1 interactor.
STRINGi316385.ECDH10B_0210.

Structurei

Secondary structure

1165
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 21Combined sources9
Helixi27 – 30Combined sources4
Helixi32 – 45Combined sources14
Beta strandi49 – 55Combined sources7
Beta strandi57 – 65Combined sources9
Helixi73 – 87Combined sources15
Beta strandi99 – 102Combined sources4
Helixi106 – 122Combined sources17
Helixi129 – 131Combined sources3
Beta strandi134 – 136Combined sources3
Helixi137 – 142Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ER8X-ray2.60A1-165[»]
ProteinModelPortaliQ47152.
SMRiQ47152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transposase 17 family. RAYT subfamily.Curated

Phylogenomic databases

eggNOGiENOG4108UK5. Bacteria.
COG1943. LUCA.
HOGENOMiHOG000117045.
InParanoidiQ47152.
KOiK07491.
PhylomeDBiQ47152.

Family and domain databases

Gene3Di3.30.70.1290. 1 hit.
InterProiView protein in InterPro
IPR002686. Transposase_17.
SMARTiView protein in SMART
SM01321. Y1_Tnp. 1 hit.
SUPFAMiSSF143422. SSF143422. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEYRRYYIK GGTWFFTVNL RNRRSQLLTT QYQMLRHAII KVKRDRPFEI
60 70 80 90 100
NAWVVLPEHM HCIWTLPEGD DDFSSRWREI KKQFTHACGL KNIWQPRFWE
110 120 130 140 150
HAIRNTKDYR HHVDYIYINP VKHGWVKQVS DWPFSTFHRD VARGLYPIDW
160
AGDVTDFSAG ERIIS
Length:165
Mass (Da):20,042
Last modified:November 1, 1997 - v1
Checksum:i970463B76841B864
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA. Translation: BAA07590.1.
U70214 Genomic DNA. Translation: AAB08648.1.
U00096 Genomic DNA. Translation: AAC73332.1.
AP009048 Genomic DNA. Translation: BAA77898.1.
PIRiE64747.
RefSeqiNP_414763.1. NC_000913.3.
WP_000006255.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73332; AAC73332; b0228.
BAA77898; BAA77898; BAA77898.
GeneIDi944913.
KEGGiecj:JW0218.
eco:b0228.
PATRICifig|1411691.4.peg.2055.

Similar proteinsi

Entry informationi

Entry nameiRAYT_ECOLI
AccessioniPrimary (citable) accession number: Q47152
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: August 30, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome, Transposable element

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families