Q47151 (YAFL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable endopeptidase YafL
EC=3.4.-.-

Alternative name(s):
Uncharacterized lipoprotein YafL

Gene names

Name:	yafL
Ordered Locus Names:	b0227, JW0217

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor Potential.
Sequence similarities	Belongs to the peptidase C40 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yhhK	P37613	1	EBI-1114812,EBI-1132877

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 249	232	Probable endopeptidase YafL		PRO_0000019767
Sites
	Active site	147	1	Nucleophile By similarity
	Active site	202	1	Proton acceptor By similarity
Amino acid modifications
	Lipidation	18	1	N-palmitoyl cysteine Potential
	Lipidation	18	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q47151 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 3C4F4618212C3466	FASTA	249	28,785
	10 20 30 40 50 60 MSLPSIPSFV LSGLLLICLP FSSFASATTS HISFSYAARQ RMQNRARLLK QYQTHLKKQA 70 80 90 100 110 120 SYIVEGNAES KRALRQHNRE QIKQHPEWFP APLKASDRRW QALAENNHFL SSDHLHNITE 130 140 150 160 170 180 VAIHRLEQQL GKPYVWGGTR PDKGFDCSGL VFYAYNKILE AKLPRTANEM YHYRRATIVA 190 200 210 220 230 240 NNDLRRGDLL FFHIHSREIA DHMGVYLGDG QFIESPRTGE TIRISRLAEP FWQDHFLGAR RILTEETIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"dinP, a new gene in Escherichia coli, whose product shows similarities to UmuC and its homologues." Ohmori H., Hatada E., Qiao Y., Tsuji M., Fukuda R. Mutat. Res. 347:1-7(1995) [PubMed: 7596361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38582 Genomic DNA. Translation: BAA07589.1. U70214 Genomic DNA. Translation: AAB08647.1. U00096 Genomic DNA. Translation: AAC73331.1. AP009048 Genomic DNA. Translation: BAA77897.2.
PIR	D64747.
RefSeq	NP_414762.1. NC_000913.2.
3D structure databases
ProteinModelPortal	Q47151.
SMR	Q47151. Positions 116-244.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q47151. 6 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002858; EBESCP00000002858; EBESCG00000002331. EBESCT00000016266; EBESCP00000015557; EBESCG00000015326.
GeneID	944899.
GenomeReviews	Gene locus JW0217 in contig AP009048_GR. Gene locus b0227 in contig U00096_GR.
KEGG	ecj:JW0217. eco:b0227.
PATRIC	32115569. VBIEscCol129921_0229.
Organism-specific databases
EchoBASE	EB2943.
EcoGene	EG13149. yafL.
Phylogenomic databases
eggNOG	COG0791.
GeneTree	EBGT00050000009578.
HOGENOM	HBG362075.
OMA	MHDQPLL.
PhylomeDB	Q47151.
ProtClustDB	CLSK879595.
Enzyme and pathway databases
BioCyc	EcoCyc:G6111-MONOMER.
Gene expression databases
Genevestigator	Q47151.
Family and domain databases
InterPro	IPR000064. NLP_P60. [Graphical view]
Pfam	PF00877. NLPC_P60. 1 hit. [Graphical view]
PROSITE	PS51257. PROKAR_LIPOPROTEIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAFL_ECOLI

Accession

Primary (citable) accession number: Q47151
Secondary accession number(s): Q9R2E0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program