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Protein

Antitoxin DinJ

Gene

dinJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a type II toxin-antitoxin (TA) module (PubMed:17263853). A labile antitoxin that counteracts the effect of cognate toxin YafQ (PubMed:17263853). YafQ and DinJ together bind their own promoter, and repress its expression (PubMed:24898247). There are 2 operators with imperfect inverted repeats (IR) in the dinJ promoter, YafQ-(DinJ)2-YafQ only binds to the first (most upstream) of them to repress transcription; binding to a single IR is sufficient for activity in vivo and in vitro (PubMed:24898247). DinJ alone is as potent a transcriptional repressor as the heterotetramer and also only needs to bind 1 IR to act (PubMed:24898247).2 Publications
Cell death governed by the MazE-MazF and DinJ-YafQ TA modules seems to play a role in biofilm formation (PubMed:19707553).1 Publication

GO - Molecular functioni

  • bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding Source: EcoCyc
  • DNA binding Source: EcoCyc
  • sequence-specific DNA binding Source: CollecTF
  • toxic substance binding Source: EcoCyc

GO - Biological processi

  • negative regulation of DNA-templated transcription, initiation Source: EcoCyc
  • single-species biofilm formation Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G6110-MONOMER.
MetaCyc:G6110-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin DinJ
Gene namesi
Name:dinJ
Ordered Locus Names:b0226, JW0216
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13142. dinJ.

Subcellular locationi

GO - Cellular componenti

  • protein-DNA complex Source: CollecTF

Pathology & Biotechi

Disruption phenotypei

Cells missing dinJ-yafQ have reduced biofilm formation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2 – 44Missing : About 50% loss of promoter repression, probably forms YafQ-DinQ dimers. 1 PublicationAdd BLAST43
Mutagenesisi2 – 12Missing : About 75% loss of promoter repression, probably forms YafQ-DinQ tetramers. 1 PublicationAdd BLAST11
Mutagenesisi10R → A: Nearly complete loss of promoter repression, YafQ-(DinJ)2-YafQ no longer binds DNA. 1 Publication1
Mutagenesisi35R → A: About 90% loss of promoter repression, YafQ-(DinJ)2-YafQ no longer binds DNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000799031 – 86Antitoxin DinJAdd BLAST86

Post-translational modificationi

Probably degraded by the Lon and ClpPX proteases in vivo.1 Publication

Proteomic databases

PaxDbiQ47150.
PRIDEiQ47150.

Expressioni

Inductioni

Not induced by the DNA damaging agent mitomycin C (PubMed:19210620). Transcription is autorepressed by DinJ or YafQ-(DinJ)2-YafQ via operator 1, repressed by LexA via operator 2 (PubMed:24898247).2 Publications

Gene expression databases

CollecTFiEXPREG_00000df0.

Interactioni

Subunit structurei

Forms a heterotetramer with antitoxin DinJ, with 2 YafQ-DinJ dimers associated via the N-terminus of the DinJ antitoxins (YafQ-(DinJ)2-YafQ) (PubMed:17263853, PubMed:24898247). In this complex the toxin activity is inhibited.3 Publications

Protein-protein interaction databases

BioGridi4259772. 55 interactors.
DIPiDIP-9446N.
IntActiQ47150. 2 interactors.
STRINGi316385.ECDH10B_0208.

Structurei

Secondary structure

186
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 11Combined sources8
Helixi13 – 24Combined sources12
Turni25 – 27Combined sources3
Helixi30 – 44Combined sources15
Helixi56 – 67Combined sources12
Turni68 – 70Combined sources3
Beta strandi71 – 76Combined sources6
Helixi77 – 84Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q2UX-ray1.80A/C/E/G/I/K/M/O1-86[»]
ProteinModelPortaliQ47150.
SMRiQ47150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RelB/DinJ antitoxin family.Curated

Phylogenomic databases

eggNOGiENOG4105QHH. Bacteria.
COG3077. LUCA.
HOGENOMiHOG000008424.
KOiK07473.

Family and domain databases

InterProiView protein in InterPro
IPR026262. DinJ.
IPR007337. RelB/DinJ.
PfamiView protein in Pfam
PF04221. RelB. 1 hit.
PIRSFiPIRSF003108. DinJ. 1 hit.
TIGRFAMsiTIGR02384. RelB_DinJ. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47150-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANAFVRAR IDEDLKNQAA DVLAGMGLTI SDLVRITLTK VAREKALPFD
60 70 80
LREPNQLTIQ SIKNSEAGID VHKAKDADDL FDKLGI
Length:86
Mass (Da):9,406
Last modified:November 1, 1997 - v1
Checksum:i9B8786E70D90B3E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38582 Genomic DNA. Translation: BAA07588.1.
U00096 Genomic DNA. Translation: AAC73330.1.
AP009048 Genomic DNA. Translation: BAA77896.1.
PIRiC64747.
RefSeqiNP_414761.1. NC_000913.3.
WP_000729703.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73330; AAC73330; b0226.
BAA77896; BAA77896; BAA77896.
GeneIDi944914.
KEGGiecj:JW0216.
eco:b0226.
PATRICifig|1411691.4.peg.2057.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDINJ_ECOLI
AccessioniPrimary (citable) accession number: Q47150
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 5, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families