Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acyl-coenzyme A dehydrogenase

Gene

fadE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the dehydrogenation of acyl-CoA.1 Publication

Catalytic activityi

An acyl-CoA + FAD = a dehydrogenated acyl-CoA + FADH2.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: EcoCyc
  • lipid homeostasis Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:ACYLCOADEHYDROG-MONOMER.
ECOL316407:JW5020-MONOMER.
MetaCyc:ACYLCOADEHYDROG-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A dehydrogenase (EC:1.3.99.-)
Short name:
ACDH
Gene namesi
Name:fadE
Synonyms:yafH
Ordered Locus Names:b0221, JW5020
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13145. fadE.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 814814Acyl-coenzyme A dehydrogenasePRO_0000201200Add
BLAST

Proteomic databases

PaxDbiQ47146.
PRIDEiQ47146.

Expressioni

Inductioni

Negatively regulated by FadR.

Interactioni

Protein-protein interaction databases

BioGridi4259770. 205 interactions.
STRINGi511145.b0221.

Structurei

3D structure databases

ProteinModelPortaliQ47146.
SMRiQ47146. Positions 109-522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4107QPN. Bacteria.
COG1960. LUCA.
HOGENOMiHOG000274434.
InParanoidiQ47146.
KOiK06445.
OMAiNDFEITH.
OrthoDBiEOG6VF2ZZ.
PhylomeDBiQ47146.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
IPR015396. DUF1974.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
PF09317. DUF1974. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMILSILATV VLLGALFYHR VSLFISSLIL LAWTAALGVA GLWSAWVLVP
60 70 80 90 100
LAIILVPFNF APMRKSMISA PVFRGFRKVM PPMSRTEKEA IDAGTTWWEG
110 120 130 140 150
DLFQGKPDWK KLHNYPQPRL TAEEQAFLDG PVEEACRMAN DFQITHELAD
160 170 180 190 200
LPPELWAYLK EHRFFAMIIK KEYGGLEFSA YAQSRVLQKL SGVSGILAIT
210 220 230 240 250
VGVPNSLGPG ELLQHYGTDE QKDHYLPRLA RGQEIPCFAL TSPEAGSDAG
260 270 280 290 300
AIPDTGIVCM GEWQGQQVLG MRLTWNKRYI TLAPIATVLG LAFKLSDPEK
310 320 330 340 350
LLGGAEDLGI TCALIPTTTP GVEIGRRHFP LNVPFQNGPT RGKDVFVPID
360 370 380 390 400
YIIGGPKMAG QGWRMLVECL SVGRGITLPS NSTGGVKSVA LATGAYAHIR
410 420 430 440 450
RQFKISIGKM EGIEEPLARI AGNAYVMDAA ASLITYGIML GEKPAVLSAI
460 470 480 490 500
VKYHCTHRGQ QSIIDAMDIT GGKGIMLGQS NFLARAYQGA PIAITVEGAN
510 520 530 540 550
ILTRSMMIFG QGAIRCHPYV LEEMEAAKNN DVNAFDKLLF KHIGHVGSNK
560 570 580 590 600
VRSFWLGLTR GLTSSTPTGD ATKRYYQHLN RLSANLALLS DVSMAVLGGS
610 620 630 640 650
LKRRERISAR LGDILSQLYL ASAVLKRYDD EGRNEADLPL VHWGVQDALY
660 670 680 690 700
QAEQAMDDLL QNFPNRVVAG LLNVVIFPTG RHYLAPSDKL DHKVAKILQV
710 720 730 740 750
PNATRSRIGR GQYLTPSEHN PVGLLEEALV DVIAADPIHQ RICKELGKNL
760 770 780 790 800
PFTRLDELAH NALVKGLIDK DEAAILVKAE ESRLRSINVD DFDPEELATK
810
PVKLPEKVRK VEAA
Length:814
Mass (Da):89,224
Last modified:November 1, 2002 - v2
Checksum:iAD9E40ACB44CF781
GO

Sequence cautioni

The sequence BAA07583.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08643.1.
U00096 Genomic DNA. Translation: AAC73325.2.
AP009048 Genomic DNA. Translation: BAA77891.2.
D38582 Genomic DNA. Translation: BAA07583.1. Different initiation.
PIRiF64746.
RefSeqiNP_414756.2. NC_000913.3.
WP_000973083.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73325; AAC73325; b0221.
BAA77891; BAA77891; BAA77891.
GeneIDi949007.
KEGGiecj:JW5020.
eco:b0221.
PATRICi32115557. VBIEscCol129921_0223.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA. Translation: AAB08643.1.
U00096 Genomic DNA. Translation: AAC73325.2.
AP009048 Genomic DNA. Translation: BAA77891.2.
D38582 Genomic DNA. Translation: BAA07583.1. Different initiation.
PIRiF64746.
RefSeqiNP_414756.2. NC_000913.3.
WP_000973083.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ47146.
SMRiQ47146. Positions 109-522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259770. 205 interactions.
STRINGi511145.b0221.

Proteomic databases

PaxDbiQ47146.
PRIDEiQ47146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73325; AAC73325; b0221.
BAA77891; BAA77891; BAA77891.
GeneIDi949007.
KEGGiecj:JW5020.
eco:b0221.
PATRICi32115557. VBIEscCol129921_0223.

Organism-specific databases

EchoBASEiEB2939.
EcoGeneiEG13145. fadE.

Phylogenomic databases

eggNOGiENOG4107QPN. Bacteria.
COG1960. LUCA.
HOGENOMiHOG000274434.
InParanoidiQ47146.
KOiK06445.
OMAiNDFEITH.
OrthoDBiEOG6VF2ZZ.
PhylomeDBiQ47146.

Enzyme and pathway databases

UniPathwayiUPA00659.
BioCyciEcoCyc:ACYLCOADEHYDROG-MONOMER.
ECOL316407:JW5020-MONOMER.
MetaCyc:ACYLCOADEHYDROG-MONOMER.

Miscellaneous databases

PROiQ47146.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
IPR015396. DUF1974.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
PF09317. DUF1974. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
    Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "dinP, a new gene in Escherichia coli, whose product shows similarities to UmuC and its homologues."
    Ohmori H., Hatada E., Qiao Y., Tsuji M., Fukuda R.
    Mutat. Res. 347:1-7(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-315.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "The enigmatic Escherichia coli fadE gene is yafH."
    Campbell J.W., Cronan J.E. Jr.
    J. Bacteriol. 184:3759-3764(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiFADE_ECOLI
AccessioniPrimary (citable) accession number: Q47146
Secondary accession number(s): P71283, P75673, Q47680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 2002
Last modified: January 20, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally named fadF.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.