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Protein

Acyl-coenzyme A dehydrogenase

Gene

fadE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the dehydrogenation of acyl-coenzymes A (acyl-CoAs) to 2-enoyl-CoAs, the first step of the beta-oxidation cycle of fatty acid degradation. Is required for E.coli to utilize dodecanoate or oleate as the sole carbon and energy source for growth.1 Publication

Caution

Was originally named fadF.Curated

Catalytic activityi

A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein.1 Publication
Long-chain-acyl-CoA + electron-transfer flavoprotein = long-chain-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein.1 Publication

Cofactori

FADBy similarity

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei497Proton acceptorBy similarity1

GO - Molecular functioni

  • acyl-CoA dehydrogenase activity Source: EcoCyc
  • flavin adenine dinucleotide binding Source: InterPro

GO - Biological processi

  • fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:ACYLCOADEHYDROG-MONOMER
MetaCyc:ACYLCOADEHYDROG-MONOMER
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A dehydrogenase1 Publication (EC:1.3.8.71 Publication, EC:1.3.8.81 Publication)
Short name:
ACDH
Short name:
Acyl-CoA dehydrogenase1 Publication
Gene namesi
Name:fadE1 Publication
Synonyms:yafH
Ordered Locus Names:b0221, JW5020
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13145 fadE

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are unable to grow on octanoate, decanoate, dodecanoate, or oleate as the sole carbon and energy source, but grow well on acetate, while the wild-type strain can grow on acetate, dodecanoate, and oleate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002012001 – 814Acyl-coenzyme A dehydrogenaseAdd BLAST814

Proteomic databases

PaxDbiQ47146
PRIDEiQ47146

Expressioni

Inductioni

Transcription of fadE is negatively regulated by FadR. Induced in the presence of fatty acids, in a FadR-dependent manner.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259770, 205 interactors
STRINGi316385.ECDH10B_0203

Structurei

3D structure databases

ProteinModelPortaliQ47146
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4107QPN Bacteria
COG1960 LUCA
HOGENOMiHOG000274434
InParanoidiQ47146
KOiK06445
OMAiPIGKMEG
PhylomeDBiQ47146

Family and domain databases

Gene3Di1.10.540.10, 1 hit
InterProiView protein in InterPro
IPR006091 Acyl-CoA_Oxase/DH_cen-dom
IPR036250 AcylCo_DH-like_C
IPR009075 AcylCo_DH/oxidase_C
IPR013786 AcylCoA_DH/ox_N
IPR037069 AcylCoA_DH/ox_N_sf
IPR009100 AcylCoA_DH/oxidase_NM_dom
IPR015396 DUF1974
PfamiView protein in Pfam
PF00441 Acyl-CoA_dh_1, 1 hit
PF02770 Acyl-CoA_dh_M, 1 hit
PF02771 Acyl-CoA_dh_N, 1 hit
PF09317 DUF1974, 1 hit
SUPFAMiSSF47203 SSF47203, 1 hit
SSF56645 SSF56645, 1 hit

Sequencei

Sequence statusi: Complete.

Q47146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMILSILATV VLLGALFYHR VSLFISSLIL LAWTAALGVA GLWSAWVLVP
60 70 80 90 100
LAIILVPFNF APMRKSMISA PVFRGFRKVM PPMSRTEKEA IDAGTTWWEG
110 120 130 140 150
DLFQGKPDWK KLHNYPQPRL TAEEQAFLDG PVEEACRMAN DFQITHELAD
160 170 180 190 200
LPPELWAYLK EHRFFAMIIK KEYGGLEFSA YAQSRVLQKL SGVSGILAIT
210 220 230 240 250
VGVPNSLGPG ELLQHYGTDE QKDHYLPRLA RGQEIPCFAL TSPEAGSDAG
260 270 280 290 300
AIPDTGIVCM GEWQGQQVLG MRLTWNKRYI TLAPIATVLG LAFKLSDPEK
310 320 330 340 350
LLGGAEDLGI TCALIPTTTP GVEIGRRHFP LNVPFQNGPT RGKDVFVPID
360 370 380 390 400
YIIGGPKMAG QGWRMLVECL SVGRGITLPS NSTGGVKSVA LATGAYAHIR
410 420 430 440 450
RQFKISIGKM EGIEEPLARI AGNAYVMDAA ASLITYGIML GEKPAVLSAI
460 470 480 490 500
VKYHCTHRGQ QSIIDAMDIT GGKGIMLGQS NFLARAYQGA PIAITVEGAN
510 520 530 540 550
ILTRSMMIFG QGAIRCHPYV LEEMEAAKNN DVNAFDKLLF KHIGHVGSNK
560 570 580 590 600
VRSFWLGLTR GLTSSTPTGD ATKRYYQHLN RLSANLALLS DVSMAVLGGS
610 620 630 640 650
LKRRERISAR LGDILSQLYL ASAVLKRYDD EGRNEADLPL VHWGVQDALY
660 670 680 690 700
QAEQAMDDLL QNFPNRVVAG LLNVVIFPTG RHYLAPSDKL DHKVAKILQV
710 720 730 740 750
PNATRSRIGR GQYLTPSEHN PVGLLEEALV DVIAADPIHQ RICKELGKNL
760 770 780 790 800
PFTRLDELAH NALVKGLIDK DEAAILVKAE ESRLRSINVD DFDPEELATK
810
PVKLPEKVRK VEAA
Length:814
Mass (Da):89,224
Last modified:November 1, 2002 - v2
Checksum:iAD9E40ACB44CF781
GO

Sequence cautioni

The sequence BAA07583 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70214 Genomic DNA Translation: AAB08643.1
U00096 Genomic DNA Translation: AAC73325.2
AP009048 Genomic DNA Translation: BAA77891.2
D38582 Genomic DNA Translation: BAA07583.1 Different initiation.
PIRiF64746
RefSeqiNP_414756.2, NC_000913.3
WP_000973083.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73325; AAC73325; b0221
BAA77891; BAA77891; BAA77891
GeneIDi949007
KEGGiecj:JW5020
eco:b0221
PATRICifig|1411691.4.peg.2062

Similar proteinsi

Entry informationi

Entry nameiFADE_ECOLI
AccessioniPrimary (citable) accession number: Q47146
Secondary accession number(s): P71283, P75673, Q47680
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 2002
Last modified: March 28, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health