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Protein

Probable 3-phenylpropionic acid transporter

Gene

hcaT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable permease involved in the uptake of 3-phenylpropionic acid.

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:HCAT-MONOMER.
ECOL316407:JW2520-MONOMER.

Protein family/group databases

TCDBi2.A.1.27.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 3-phenylpropionic acid transporter
Gene namesi
Name:hcaT
Synonyms:yfhS
Ordered Locus Names:b2536, JW2520
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13454. hcaT.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicBy similarity4
Transmembranei5 – 31Helical; Name=1By similarityAdd BLAST27
Topological domaini32 – 37PeriplasmicBy similarity6
Transmembranei38 – 66Helical; Name=2By similarityAdd BLAST29
Topological domaini67 – 70CytoplasmicBy similarity4
Transmembranei71 – 96Helical; Name=3By similarityAdd BLAST26
Topological domaini97 – 100PeriplasmicBy similarity4
Transmembranei101 – 118Helical; Name=4By similarityAdd BLAST18
Topological domaini119 – 129CytoplasmicBy similarityAdd BLAST11
Transmembranei130 – 152Helical; Name=5By similarityAdd BLAST23
Topological domaini153 – 155PeriplasmicBy similarity3
Transmembranei156 – 175Helical; Name=6By similarityAdd BLAST20
Topological domaini176 – 207CytoplasmicBy similarityAdd BLAST32
Transmembranei208 – 227Helical; Name=7By similarityAdd BLAST20
Topological domaini228 – 231PeriplasmicBy similarity4
Transmembranei232 – 256Helical; Name=8By similarityAdd BLAST25
Topological domaini257 – 266CytoplasmicBy similarity10
Transmembranei267 – 286Helical; Name=9By similarityAdd BLAST20
Topological domaini287 – 289PeriplasmicBy similarity3
Transmembranei290 – 312Helical; Name=10By similarityAdd BLAST23
Topological domaini313 – 323CytoplasmicBy similarityAdd BLAST11
Transmembranei324 – 351Helical; Name=11By similarityAdd BLAST28
Topological domaini352 – 354PeriplasmicBy similarity3
Transmembranei355 – 375Helical; Name=12By similarityAdd BLAST21
Topological domaini376 – 379CytoplasmicBy similarity4

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001961891 – 379Probable 3-phenylpropionic acid transporterAdd BLAST379

Proteomic databases

PaxDbiQ47142.
PRIDEiQ47142.

Interactioni

Protein-protein interaction databases

BioGridi4261387. 11 interactors.
STRINGi511145.b2536.

Structurei

3D structure databases

ProteinModelPortaliQ47142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D8G. Bacteria.
ENOG410XQVS. LUCA.
HOGENOMiHOG000272903.
InParanoidiQ47142.
KOiK05820.
OMAiMDYGRVR.
PhylomeDBiQ47142.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR026032. HcaT.
IPR024989. MFS_assoc_dom.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF12832. MFS_1_like. 1 hit.
[Graphical view]
PIRSFiPIRSF004925. HcaT. 1 hit.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00902. 2A0127. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47142-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLQSTRWLA LGYFTYFFSY GIFLPFWSVW LKGIGLTPET IGLLLGAGLV
60 70 80 90 100
ARFLGSLLIA PRVSDPSRLI SALRVLALLT LLFAVAFWAG AHVAWLMLVM
110 120 130 140 150
IGFNLFFSPL VPLTDALANT WQKQFPLDYG KVRLWGSVAF VIGSALTGKL
160 170 180 190 200
VTMFDYRVIL ALLTLGVASM LLGFLIRPTI QPQGASRQQE STGWSAWLAL
210 220 230 240 250
VRQNWRFLAC VCLLQGAHAA YYGFSAIYWQ AAGYSASAVG YLWSLGVVAE
260 270 280 290 300
VIIFALSNKL FRRCSARDML LISAICGVVR WGIMGATTAL PWLIVVQILH
310 320 330 340 350
CGTFTVCHLA AMRYIAARQG SEVIRLQAVY SAVAMGGSIA IMTVFAGFLY
360 370
QYLGHGVFWV MALVALPAMF LRPKVVPSC
Length:379
Mass (Da):41,593
Last modified:November 1, 1997 - v2
Checksum:i76429AD807623482
GO

Sequence cautioni

The sequence CAA86021 differs from that shown. Reason: Frameshift at position 208.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191S → N in CAA71954 (PubMed:9603882).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37966 Genomic DNA. Translation: CAA86021.1. Frameshift.
Y11071 Genomic DNA. Translation: CAA71954.1.
U00096 Genomic DNA. Translation: AAC75589.1.
AP009048 Genomic DNA. Translation: BAA16431.1.
PIRiG65030.
S49295.
RefSeqiNP_417031.1. NC_000913.3.
WP_000244193.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75589; AAC75589; b2536.
BAA16431; BAA16431; BAA16431.
GeneIDi947007.
KEGGiecj:JW2520.
eco:b2536.
PATRICi32120469. VBIEscCol129921_2637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37966 Genomic DNA. Translation: CAA86021.1. Frameshift.
Y11071 Genomic DNA. Translation: CAA71954.1.
U00096 Genomic DNA. Translation: AAC75589.1.
AP009048 Genomic DNA. Translation: BAA16431.1.
PIRiG65030.
S49295.
RefSeqiNP_417031.1. NC_000913.3.
WP_000244193.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ47142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261387. 11 interactors.
STRINGi511145.b2536.

Protein family/group databases

TCDBi2.A.1.27.1. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiQ47142.
PRIDEiQ47142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75589; AAC75589; b2536.
BAA16431; BAA16431; BAA16431.
GeneIDi947007.
KEGGiecj:JW2520.
eco:b2536.
PATRICi32120469. VBIEscCol129921_2637.

Organism-specific databases

EchoBASEiEB3227.
EcoGeneiEG13454. hcaT.

Phylogenomic databases

eggNOGiENOG4105D8G. Bacteria.
ENOG410XQVS. LUCA.
HOGENOMiHOG000272903.
InParanoidiQ47142.
KOiK05820.
OMAiMDYGRVR.
PhylomeDBiQ47142.

Enzyme and pathway databases

BioCyciEcoCyc:HCAT-MONOMER.
ECOL316407:JW2520-MONOMER.

Miscellaneous databases

PROiQ47142.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR026032. HcaT.
IPR024989. MFS_assoc_dom.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF12832. MFS_1_like. 1 hit.
[Graphical view]
PIRSFiPIRSF004925. HcaT. 1 hit.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00902. 2A0127. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHCAT_ECOLI
AccessioniPrimary (citable) accession number: Q47142
Secondary accession number(s): O05660, P77638
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.