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Protein

Hca operon transcriptional activator HcaR

Gene

hcaR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator of the hca operon for 3-phenylpropionic acid catabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi18 – 38H-T-H motifPROSITE-ProRule annotationAdd BLAST21

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7331-MONOMER.
ECOL316407:JW2521-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hca operon transcriptional activator HcaR
Gene namesi
Name:hcaR
Synonyms:phdR, yfhT
Ordered Locus Names:b2537, JW2521
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13455. hcaR.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001056311 – 296Hca operon transcriptional activator HcaRAdd BLAST296

Proteomic databases

PaxDbiQ47141.
PRIDEiQ47141.

Interactioni

Protein-protein interaction databases

BioGridi4261574. 17 interactors.
IntActiQ47141. 2 interactors.
STRINGi511145.b2537.

Structurei

3D structure databases

ProteinModelPortaliQ47141.
SMRiQ47141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58HTH lysR-typePROSITE-ProRule annotationAdd BLAST58

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108W5M. Bacteria.
ENOG4111IYC. LUCA.
HOGENOMiHOG000233514.
InParanoidiQ47141.
KOiK05817.
OMAiGFGRIRY.
PhylomeDBiQ47141.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELRHLRYFV AVAQALNFTR AAEKLHTSQP SLSSQIRDLE NCVGVPLLVR
60 70 80 90 100
DKRKVALTAA GECFLQDALA ILEQAENAKL RARKIVQEDR QLTIGFVPSA
110 120 130 140 150
EVNLLPKVLP MFRLRQPDTL IELVSLITTQ QEEKIRRGEL DVGLMRHPVY
160 170 180 190 200
SPEIDYLELF DEPLVVVLPV DHPLAHEKEI TAAQLDGVNF VSTDPVYSGS
210 220 230 240 250
LAPIVKAWFA QENSQPNIVQ VATNILVTMN LVGMGLGVTL IPGYMNNFNT
260 270 280 290
GQVVFRPIAG NVPSIALLMA WKKGEMKPAL RDFIAIVQER LASVTA
Length:296
Mass (Da):32,838
Last modified:November 1, 1997 - v2
Checksum:i72FF08A62D490AAA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127 – 141ITTQQ…RGELD → SPRNRRKKFAVVNST in CAA86020 (Ref. 1) CuratedAdd BLAST15
Sequence conflicti145 – 148MRHP → NCAIH in CAA86020 (Ref. 1) Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37966 Genomic DNA. Translation: CAA86020.1.
U00096 Genomic DNA. Translation: AAC75590.1.
AP009048 Genomic DNA. Translation: BAA16432.2.
PIRiH65030.
RefSeqiNP_417032.1. NC_000913.3.
WP_000423261.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75590; AAC75590; b2537.
BAA16432; BAA16432; BAA16432.
GeneIDi947000.
KEGGiecj:JW2521.
eco:b2537.
PATRICi32120471. VBIEscCol129921_2638.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37966 Genomic DNA. Translation: CAA86020.1.
U00096 Genomic DNA. Translation: AAC75590.1.
AP009048 Genomic DNA. Translation: BAA16432.2.
PIRiH65030.
RefSeqiNP_417032.1. NC_000913.3.
WP_000423261.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ47141.
SMRiQ47141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261574. 17 interactors.
IntActiQ47141. 2 interactors.
STRINGi511145.b2537.

Proteomic databases

PaxDbiQ47141.
PRIDEiQ47141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75590; AAC75590; b2537.
BAA16432; BAA16432; BAA16432.
GeneIDi947000.
KEGGiecj:JW2521.
eco:b2537.
PATRICi32120471. VBIEscCol129921_2638.

Organism-specific databases

EchoBASEiEB3228.
EcoGeneiEG13455. hcaR.

Phylogenomic databases

eggNOGiENOG4108W5M. Bacteria.
ENOG4111IYC. LUCA.
HOGENOMiHOG000233514.
InParanoidiQ47141.
KOiK05817.
OMAiGFGRIRY.
PhylomeDBiQ47141.

Enzyme and pathway databases

BioCyciEcoCyc:G7331-MONOMER.
ECOL316407:JW2521-MONOMER.

Miscellaneous databases

PROiQ47141.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHCAR_ECOLI
AccessioniPrimary (citable) accession number: Q47141
Secondary accession number(s): P76583, P77167
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The 21 C-terminal amino acids have been proposed to be expressed from an independent promoter within this gene and called IroK. Overexpression of IroK has been suggested to be responsible for 3-hydroxypropionic acid (3-HP) resistance. However the IroK peptide's existence has not been proven, nor has mutagenesis of HcaR itself rather than overexpression of IroK been ruled out as the cause of 3-HP resistance (PubMed:22161628).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.