Q47141 (HCAR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hca operon transcriptional activator

Gene names

Name:	hcaR
Synonyms:	phdR, yfhT
Ordered Locus Names:	b2537, JW2521

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator of the hca operon for 3-phenylpropionic acid catabolism.
Sequence similarities	Contains 1 HTH lysR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 11200225. Source: EcoCyc positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 11200225. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from direct assay PubMed 15808934. Source: EcoliWiki sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 11200225. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Hca operon transcriptional activator		PRO_0000105631
Regions
	Domain	1 – 58	58	HTH lysR-type
	DNA binding	18 – 38	21	H-T-H motif Potential
Experimental info
	Sequence conflict	127 – 141	15	ITTQQ…RGELD → SPRNRRKKFAVVNST Ref.1
	Sequence conflict	145 – 148	4	MRHP → NCAIH Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q47141 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 72FF08A62D490AAA	FASTA	296	32,838
	10 20 30 40 50 60 MELRHLRYFV AVAQALNFTR AAEKLHTSQP SLSSQIRDLE NCVGVPLLVR DKRKVALTAA 70 80 90 100 110 120 GECFLQDALA ILEQAENAKL RARKIVQEDR QLTIGFVPSA EVNLLPKVLP MFRLRQPDTL 130 140 150 160 170 180 IELVSLITTQ QEEKIRRGEL DVGLMRHPVY SPEIDYLELF DEPLVVVLPV DHPLAHEKEI 190 200 210 220 230 240 TAAQLDGVNF VSTDPVYSGS LAPIVKAWFA QENSQPNIVQ VATNILVTMN LVGMGLGVTL 250 260 270 280 290 IPGYMNNFNT GQVVFRPIAG NVPSIALLMA WKKGEMKPAL RDFIAIVQER LASVTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Turlin E., Gasser F., Biville F. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12." Diaz E., Ferrandez A., Garcia J.L. J. Bacteriol. 180:2915-2923(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z37966 Genomic DNA. Translation: CAA86020.1. U00096 Genomic DNA. Translation: AAC75590.1. AP009048 Genomic DNA. Translation: BAA16432.2.
PIR	H65030.
RefSeq	NP_417032.1. NC_000913.2. YP_490765.1. NC_007779.1.
3D structure databases
ProteinModelPortal	Q47141.
SMR	Q47141. Positions 1-288.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q47141. 2 interactions.
STRING	511145.b2537.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75590; AAC75590; b2537. BAA16432; BAA16432; BAA16432.
GeneID	12932580. 947000.
KEGG	ecj:Y75_p2490. eco:b2537.
PATRIC	32120471. VBIEscCol129921_2638.
Organism-specific databases
EchoBASE	EB3228.
EcoGene	EG13455. hcaR.
Phylogenomic databases
eggNOG	COG0583.
HOGENOM	HOG000233514.
KO	K05817.
OMA	GSVERHI.
ProtClustDB	PRK09906.
Enzyme and pathway databases
BioCyc	EcoCyc:G7331-MONOMER. ECOL316407:JW2521-MONOMER.
Gene expression databases
Genevestigator	Q47141.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR005119. LysR_subst-bd. IPR000847. Tscrpt_reg_HTH_LysR. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00126. HTH_1. 1 hit. PF03466. LysR_substrate. 1 hit. [Graphical view]
PRINTS	PR00039. HTHLYSR.
PROSITE	PS50931. HTH_LYSR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCAR_ECOLI

Accession

Primary (citable) accession number: Q47141
Secondary accession number(s): P76583, P77167

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program