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Protein

3-phenylpropionate/cinnamic acid dioxygenase subunit beta

Gene

hcaF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.1 Publication

Catalytic activityi

3-phenylpropanoate + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+.
(2E)-3-phenylprop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD+.

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

GO - Molecular functioni

GO - Biological processi

  • 3-phenylpropionate catabolic process Source: EcoCyc

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processAromatic hydrocarbons catabolism
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:HCAA2-MONOMER
MetaCyc:HCAA2-MONOMER
UniPathwayiUPA00714

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase subunit beta (EC:1.14.12.19)
Gene namesi
Name:hcaF
Synonyms:digB, hcaA2, hcaB, phdC2, yfhV
Ordered Locus Names:b2539, JW2523
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13457 hcaF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000850771 – 1723-phenylpropionate/cinnamic acid dioxygenase subunit betaAdd BLAST172

Proteomic databases

PaxDbiQ47140
PRIDEiQ47140

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).

Protein-protein interaction databases

BioGridi4261576, 29 interactors
STRINGi316385.ECDH10B_2706

Structurei

3D structure databases

ProteinModelPortaliQ47140
SMRiQ47140
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108SBK Bacteria
COG5517 LUCA
HOGENOMiHOG000106036
InParanoidiQ47140
KOiK05709
OMAiLQAHQFL

Family and domain databases

CDDicd00667 ring_hydroxylating_dioxygenase, 1 hit
HAMAPiMF_01649 HcaF, 1 hit
InterProiView protein in InterPro
IPR023712 HcaF
IPR032710 NTF2-like_dom_sf
IPR000391 Rng_hydr_dOase-bsu
PANTHERiPTHR41534 PTHR41534, 1 hit
PfamiView protein in Pfam
PF00866 Ring_hydroxyl_B, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit

Sequencei

Sequence statusi: Complete.

Q47140-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAQVSLELH HRISQFLFHE ASLLDDWKFR DWLAQLDEEI RYTMRTTVNA
60 70 80 90 100
QTRDRRKGVQ PPTTWIFNDT KDQLERRIAR LETGMAWAEE PPSRTRHLIS
110 120 130 140 150
NCQISETDIP NVFAVRVNYL LYRAQKERDE TFYVGTRFDK VRRLEDDNWR
160 170
LLERDIVLDQ AVITSHNLSV LF
Length:172
Mass (Da):20,579
Last modified:November 1, 1997 - v2
Checksum:i83833B22A75BE546
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11H → Y in CAA86019 (Ref. 4) Curated1
Sequence conflicti49N → D in CAA86019 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75592.1
AP009048 Genomic DNA Translation: BAA16442.1
Z37966 Genomic DNA Translation: CAA86019.1
Y11070 Genomic DNA Translation: CAA71949.1
PIRiB65031
RefSeqiNP_417034.1, NC_000913.3
WP_001276072.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75592; AAC75592; b2539
BAA16442; BAA16442; BAA16442
GeneIDi946997
KEGGiecj:JW2523
eco:b2539
PATRICifig|1411691.4.peg.4195

Similar proteinsi

Entry informationi

Entry nameiHCAF_ECOLI
AccessioniPrimary (citable) accession number: Q47140
Secondary accession number(s): P77508
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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