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Q47112

- CEA7_ECOLX

UniProt

Q47112 - CEA7_ECOLX

Protein

Colicin-E7

Gene

colE7

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
    Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi544 – 5441Zinc
    Metal bindingi569 – 5691Zinc
    Metal bindingi573 – 5731Zinc

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cytolysis Source: UniProtKB-KW
    2. defense response to bacterium Source: UniProtKB-KW
    3. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Colicin-E7 (EC:3.1.-.-)
    Gene namesi
    Name:colE7
    Synonyms:cea
    Encoded oniPlasmid ColE70 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Subcellular locationi

    GO - Cellular componenti

    1. extrachromosomal circular DNA Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 576576Colicin-E7PRO_0000218682Add
    BLAST

    Proteomic databases

    PRIDEiQ47112.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    immQ037082EBI-1035016,EBI-1035025

    Protein-protein interaction databases

    DIPiDIP-16990N.
    IntActiQ47112. 1 interaction.
    MINTiMINT-95495.

    Structurei

    Secondary structure

    1
    576
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi89 – 913
    Beta strandi97 – 1026
    Helixi109 – 1168
    Beta strandi127 – 1348
    Helixi136 – 1427
    Beta strandi149 – 1557
    Helixi156 – 1583
    Helixi164 – 1663
    Beta strandi173 – 1775
    Beta strandi179 – 1857
    Beta strandi188 – 19710
    Beta strandi199 – 2057
    Beta strandi214 – 2196
    Beta strandi222 – 2298
    Beta strandi243 – 2453
    Turni258 – 2614
    Beta strandi262 – 2687
    Helixi271 – 2733
    Beta strandi278 – 2847
    Helixi288 – 30720
    Beta strandi447 – 4493
    Beta strandi451 – 4544
    Turni462 – 4698
    Helixi478 – 4847
    Beta strandi488 – 4914
    Helixi492 – 50413
    Helixi507 – 5104
    Helixi515 – 5228
    Helixi531 – 5333
    Beta strandi542 – 5476
    Helixi549 – 5513
    Beta strandi555 – 5573
    Helixi558 – 5603
    Beta strandi561 – 5644
    Helixi566 – 5727
    Turni573 – 5753

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M08X-ray2.10A/B447-576[»]
    1MZ8X-ray2.00B/D446-576[»]
    1PT3X-ray2.50A/B449-576[»]
    1UJZX-ray2.10B446-573[»]
    1ZNSX-ray2.50A444-576[»]
    1ZNVX-ray2.00B/D444-576[»]
    2AXCX-ray1.70A60-316[»]
    2ERHX-ray2.00B447-573[»]
    2IVHX-ray2.80A449-576[»]
    2JAZX-ray2.03B/D446-576[»]
    2JB0X-ray1.91B446-576[»]
    2JBGX-ray2.20B/D446-576[»]
    3FBDX-ray2.90A/D445-576[»]
    3GJNX-ray2.48B/C446-576[»]
    3GKLX-ray2.20A/B446-576[»]
    3ZFKX-ray1.70A/B450-573[»]
    7CEIX-ray2.30B371-576[»]
    ProteinModelPortaliQ47112.
    SMRiQ47112. Positions 77-576.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ47112.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the colicin/pyosin nuclease family.Curated

    Family and domain databases

    Gene3Di1.10.287.620. 1 hit.
    3.90.540.10. 1 hit.
    InterProiIPR024575. Cloacin_colicin_fam.
    IPR024622. Colicin/pyocin_DNase_dom.
    IPR024566. Colicin_R_dom.
    IPR003615. HNH_nuc.
    IPR016128. Pyosin/cloacin_T_dom.
    [Graphical view]
    PfamiPF03515. Cloacin. 1 hit.
    PF12639. Colicin-DNase. 1 hit.
    PF11570. E2R135. 1 hit.
    [Graphical view]
    PRINTSiPR01295. CLOACIN.
    SMARTiSM00507. HNHc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69369. SSF69369. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q47112-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGGDGRGHN SGAHNTGGNI NGGPTGLGGN GGASDGSGWS SENNPWGGGS    50
    GSGVHWGGGS GHGNGGGNSN SGGGSNSSVA APMAFGFPAL AAPGAGTLGI 100
    SVSGEALSAA IADIFAALKG PFKFSAWGIA LYGILPSEIA KDDPNMMSKI 150
    VTSLPAETVT NVQVSTLPLD QATVSVTKRV TDVVKDTRQH IAVVAGVPMS 200
    VPVVNAKPTR TPGVFHASFP GVPSLTVSTV KGLPVSTTLP RGITEDKGRT 250
    AVPAGFTFGG GSHEAVIRFP KESGQKPVYV SVTDVLTPAQ VKQRQDEEKR 300
    LQQEWNDAHP VEVAERNYEQ ARAELNQANK DVARNQERQA KAVQVYNSRK 350
    SELDAANKTL ADAKAEIKQF ERFAREPMAA GHRMWQMAGL KAQRAQTDVN 400
    NKKAAFDAAA KEKSDADVAL SSALERRKQK ENKEKDAKAK LDKESKRNKP 450
    GKATGKGKPV NNKWLNNAGK DLGSPVPDRI ANKLRDKEFK SFDDFRKKFW 500
    EEVSKDPELS KQFSRNNNDR MKVGKAPKTR TQDVSGKRTS FELHHEKPIS 550
    QNGGVYDMDN ISVVTPKRHI DIHRGK 576
    Length:576
    Mass (Da):61,350
    Last modified:May 30, 2000 - v2
    Checksum:iE5B05E73B2E17249
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62409 Genomic DNA. Translation: AAA98054.1.
    X63620 Genomic DNA. Translation: CAA45164.1.
    PIRiS22453.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62409 Genomic DNA. Translation: AAA98054.1 .
    X63620 Genomic DNA. Translation: CAA45164.1 .
    PIRi S22453.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M08 X-ray 2.10 A/B 447-576 [» ]
    1MZ8 X-ray 2.00 B/D 446-576 [» ]
    1PT3 X-ray 2.50 A/B 449-576 [» ]
    1UJZ X-ray 2.10 B 446-573 [» ]
    1ZNS X-ray 2.50 A 444-576 [» ]
    1ZNV X-ray 2.00 B/D 444-576 [» ]
    2AXC X-ray 1.70 A 60-316 [» ]
    2ERH X-ray 2.00 B 447-573 [» ]
    2IVH X-ray 2.80 A 449-576 [» ]
    2JAZ X-ray 2.03 B/D 446-576 [» ]
    2JB0 X-ray 1.91 B 446-576 [» ]
    2JBG X-ray 2.20 B/D 446-576 [» ]
    3FBD X-ray 2.90 A/D 445-576 [» ]
    3GJN X-ray 2.48 B/C 446-576 [» ]
    3GKL X-ray 2.20 A/B 446-576 [» ]
    3ZFK X-ray 1.70 A/B 450-573 [» ]
    7CEI X-ray 2.30 B 371-576 [» ]
    ProteinModelPortali Q47112.
    SMRi Q47112. Positions 77-576.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-16990N.
    IntActi Q47112. 1 interaction.
    MINTi MINT-95495.

    Proteomic databases

    PRIDEi Q47112.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q47112.

    Family and domain databases

    Gene3Di 1.10.287.620. 1 hit.
    3.90.540.10. 1 hit.
    InterProi IPR024575. Cloacin_colicin_fam.
    IPR024622. Colicin/pyocin_DNase_dom.
    IPR024566. Colicin_R_dom.
    IPR003615. HNH_nuc.
    IPR016128. Pyosin/cloacin_T_dom.
    [Graphical view ]
    Pfami PF03515. Cloacin. 1 hit.
    PF12639. Colicin-DNase. 1 hit.
    PF11570. E2R135. 1 hit.
    [Graphical view ]
    PRINTSi PR01295. CLOACIN.
    SMARTi SM00507. HNHc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69369. SSF69369. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the cea gene of the ColE7 plasmid."
      Soong B.W., Lu F.M., Chak K.-F.
      Mol. Gen. Genet. 233:177-183(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K317.
    2. "Nucleotide sequence encoding the immunity and lysis proteins and the carboxyl-terminal peptides of colicins E4 and E7."
      Lau P.C.K., Parsons M.
      Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 371-576.
      Strain: K317.
    3. "The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein."
      Ko T.P., Liao C.C., Ku W.-Y., Chak K.-F., Yuan H.S.
      Structure 7:91-102(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 371-576.

    Entry informationi

    Entry nameiCEA7_ECOLX
    AccessioniPrimary (citable) accession number: Q47112
    Secondary accession number(s): Q51604
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3