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Q47112

- CEA7_ECOLX

UniProt

Q47112 - CEA7_ECOLX

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Protein
Colicin-E7
Gene
colE7, cea
Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi544 – 5441Zinc
Metal bindingi569 – 5691Zinc
Metal bindingi573 – 5731Zinc

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: IntAct

GO - Biological processi

  1. cytolysis Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E7 (EC:3.1.-.-)
Gene namesi
Name:colE7
Synonyms:cea
Encoded oniPlasmid ColE70 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  1. extrachromosomal circular DNA Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Colicin-E7
PRO_0000218682Add
BLAST

Proteomic databases

PRIDEiQ47112.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
immQ037082EBI-1035016,EBI-1035025

Protein-protein interaction databases

DIPiDIP-16990N.
IntActiQ47112. 1 interaction.
MINTiMINT-95495.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi89 – 913
Beta strandi97 – 1026
Helixi109 – 1168
Beta strandi127 – 1348
Helixi136 – 1427
Beta strandi149 – 1557
Helixi156 – 1583
Helixi164 – 1663
Beta strandi173 – 1775
Beta strandi179 – 1857
Beta strandi188 – 19710
Beta strandi199 – 2057
Beta strandi214 – 2196
Beta strandi222 – 2298
Beta strandi243 – 2453
Turni258 – 2614
Beta strandi262 – 2687
Helixi271 – 2733
Beta strandi278 – 2847
Helixi288 – 30720
Beta strandi447 – 4493
Beta strandi451 – 4544
Turni462 – 4698
Helixi478 – 4847
Beta strandi488 – 4914
Helixi492 – 50413
Helixi507 – 5104
Helixi515 – 5228
Helixi531 – 5333
Beta strandi542 – 5476
Helixi549 – 5513
Beta strandi555 – 5573
Helixi558 – 5603
Beta strandi561 – 5644
Helixi566 – 5727
Turni573 – 5753

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M08X-ray2.10A/B447-576[»]
1MZ8X-ray2.00B/D446-576[»]
1PT3X-ray2.50A/B449-576[»]
1UJZX-ray2.10B446-573[»]
1ZNSX-ray2.50A444-576[»]
1ZNVX-ray2.00B/D444-576[»]
2AXCX-ray1.70A60-316[»]
2ERHX-ray2.00B447-573[»]
2IVHX-ray2.80A449-576[»]
2JAZX-ray2.03B/D446-576[»]
2JB0X-ray1.91B446-576[»]
2JBGX-ray2.20B/D446-576[»]
3FBDX-ray2.90A/D445-576[»]
3GJNX-ray2.48B/C446-576[»]
3GKLX-ray2.20A/B446-576[»]
3ZFKX-ray1.70A/B450-573[»]
7CEIX-ray2.30B371-576[»]
ProteinModelPortaliQ47112.
SMRiQ47112. Positions 77-576.

Miscellaneous databases

EvolutionaryTraceiQ47112.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMiSSF69369. SSF69369. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47112-1 [UniParc]FASTAAdd to Basket

« Hide

MSGGDGRGHN SGAHNTGGNI NGGPTGLGGN GGASDGSGWS SENNPWGGGS    50
GSGVHWGGGS GHGNGGGNSN SGGGSNSSVA APMAFGFPAL AAPGAGTLGI 100
SVSGEALSAA IADIFAALKG PFKFSAWGIA LYGILPSEIA KDDPNMMSKI 150
VTSLPAETVT NVQVSTLPLD QATVSVTKRV TDVVKDTRQH IAVVAGVPMS 200
VPVVNAKPTR TPGVFHASFP GVPSLTVSTV KGLPVSTTLP RGITEDKGRT 250
AVPAGFTFGG GSHEAVIRFP KESGQKPVYV SVTDVLTPAQ VKQRQDEEKR 300
LQQEWNDAHP VEVAERNYEQ ARAELNQANK DVARNQERQA KAVQVYNSRK 350
SELDAANKTL ADAKAEIKQF ERFAREPMAA GHRMWQMAGL KAQRAQTDVN 400
NKKAAFDAAA KEKSDADVAL SSALERRKQK ENKEKDAKAK LDKESKRNKP 450
GKATGKGKPV NNKWLNNAGK DLGSPVPDRI ANKLRDKEFK SFDDFRKKFW 500
EEVSKDPELS KQFSRNNNDR MKVGKAPKTR TQDVSGKRTS FELHHEKPIS 550
QNGGVYDMDN ISVVTPKRHI DIHRGK 576
Length:576
Mass (Da):61,350
Last modified:May 30, 2000 - v2
Checksum:iE5B05E73B2E17249
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62409 Genomic DNA. Translation: AAA98054.1.
X63620 Genomic DNA. Translation: CAA45164.1.
PIRiS22453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62409 Genomic DNA. Translation: AAA98054.1 .
X63620 Genomic DNA. Translation: CAA45164.1 .
PIRi S22453.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1M08 X-ray 2.10 A/B 447-576 [» ]
1MZ8 X-ray 2.00 B/D 446-576 [» ]
1PT3 X-ray 2.50 A/B 449-576 [» ]
1UJZ X-ray 2.10 B 446-573 [» ]
1ZNS X-ray 2.50 A 444-576 [» ]
1ZNV X-ray 2.00 B/D 444-576 [» ]
2AXC X-ray 1.70 A 60-316 [» ]
2ERH X-ray 2.00 B 447-573 [» ]
2IVH X-ray 2.80 A 449-576 [» ]
2JAZ X-ray 2.03 B/D 446-576 [» ]
2JB0 X-ray 1.91 B 446-576 [» ]
2JBG X-ray 2.20 B/D 446-576 [» ]
3FBD X-ray 2.90 A/D 445-576 [» ]
3GJN X-ray 2.48 B/C 446-576 [» ]
3GKL X-ray 2.20 A/B 446-576 [» ]
3ZFK X-ray 1.70 A/B 450-573 [» ]
7CEI X-ray 2.30 B 371-576 [» ]
ProteinModelPortali Q47112.
SMRi Q47112. Positions 77-576.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-16990N.
IntActi Q47112. 1 interaction.
MINTi MINT-95495.

Proteomic databases

PRIDEi Q47112.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q47112.

Family and domain databases

Gene3Di 1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProi IPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view ]
Pfami PF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view ]
PRINTSi PR01295. CLOACIN.
SMARTi SM00507. HNHc. 1 hit.
[Graphical view ]
SUPFAMi SSF69369. SSF69369. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the cea gene of the ColE7 plasmid."
    Soong B.W., Lu F.M., Chak K.-F.
    Mol. Gen. Genet. 233:177-183(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K317.
  2. "Nucleotide sequence encoding the immunity and lysis proteins and the carboxyl-terminal peptides of colicins E4 and E7."
    Lau P.C.K., Parsons M.
    Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 371-576.
    Strain: K317.
  3. "The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein."
    Ko T.P., Liao C.C., Ku W.-Y., Chak K.-F., Yuan H.S.
    Structure 7:91-102(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 371-576.

Entry informationi

Entry nameiCEA7_ECOLX
AccessioniPrimary (citable) accession number: Q47112
Secondary accession number(s): Q51604
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: June 11, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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