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Q47112 (CEA7_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Colicin-E7

EC=3.1.-.-
Gene names
Name:colE7
Synonyms:cea
Encoded onPlasmid ColE7
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.

Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sequence similarities

Belongs to the colicin/pyosin nuclease family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

immQ037082EBI-1035016,EBI-1035025

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Colicin-E7
PRO_0000218682

Sites

Metal binding5441Zinc
Metal binding5691Zinc
Metal binding5731Zinc

Secondary structure

................................................................... 576
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q47112 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: E5B05E73B2E17249

FASTA57661,350
        10         20         30         40         50         60 
MSGGDGRGHN SGAHNTGGNI NGGPTGLGGN GGASDGSGWS SENNPWGGGS GSGVHWGGGS 

        70         80         90        100        110        120 
GHGNGGGNSN SGGGSNSSVA APMAFGFPAL AAPGAGTLGI SVSGEALSAA IADIFAALKG 

       130        140        150        160        170        180 
PFKFSAWGIA LYGILPSEIA KDDPNMMSKI VTSLPAETVT NVQVSTLPLD QATVSVTKRV 

       190        200        210        220        230        240 
TDVVKDTRQH IAVVAGVPMS VPVVNAKPTR TPGVFHASFP GVPSLTVSTV KGLPVSTTLP 

       250        260        270        280        290        300 
RGITEDKGRT AVPAGFTFGG GSHEAVIRFP KESGQKPVYV SVTDVLTPAQ VKQRQDEEKR 

       310        320        330        340        350        360 
LQQEWNDAHP VEVAERNYEQ ARAELNQANK DVARNQERQA KAVQVYNSRK SELDAANKTL 

       370        380        390        400        410        420 
ADAKAEIKQF ERFAREPMAA GHRMWQMAGL KAQRAQTDVN NKKAAFDAAA KEKSDADVAL 

       430        440        450        460        470        480 
SSALERRKQK ENKEKDAKAK LDKESKRNKP GKATGKGKPV NNKWLNNAGK DLGSPVPDRI 

       490        500        510        520        530        540 
ANKLRDKEFK SFDDFRKKFW EEVSKDPELS KQFSRNNNDR MKVGKAPKTR TQDVSGKRTS 

       550        560        570 
FELHHEKPIS QNGGVYDMDN ISVVTPKRHI DIHRGK 

« Hide

References

[1]"Characterization of the cea gene of the ColE7 plasmid."
Soong B.W., Lu F.M., Chak K.-F.
Mol. Gen. Genet. 233:177-183(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K317.
[2]"Nucleotide sequence encoding the immunity and lysis proteins and the carboxyl-terminal peptides of colicins E4 and E7."
Lau P.C.K., Parsons M.
Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 371-576.
Strain: K317.
[3]"The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein."
Ko T.P., Liao C.C., Ku W.-Y., Chak K.-F., Yuan H.S.
Structure 7:91-102(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 371-576.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M62409 Genomic DNA. Translation: AAA98054.1.
X63620 Genomic DNA. Translation: CAA45164.1.
PIRS22453.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M08X-ray2.10A/B447-576[»]
1MZ8X-ray2.00B/D446-576[»]
1PT3X-ray2.50A/B449-576[»]
1UJZX-ray2.10B446-573[»]
1ZNSX-ray2.50A444-576[»]
1ZNVX-ray2.00B/D444-576[»]
2AXCX-ray1.70A60-316[»]
2ERHX-ray2.00B447-573[»]
2IVHX-ray2.80A449-576[»]
2JAZX-ray2.03B/D446-576[»]
2JB0X-ray1.91B446-576[»]
2JBGX-ray2.20B/D446-576[»]
3FBDX-ray2.90A/D445-576[»]
3GJNX-ray2.48B/C446-576[»]
3GKLX-ray2.20A/B446-576[»]
3ZFKX-ray1.70A/B450-573[»]
7CEIX-ray2.30B371-576[»]
ProteinModelPortalQ47112.
SMRQ47112. Positions 77-576.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-16990N.
IntActQ47112. 1 interaction.
MINTMINT-95495.

Proteomic databases

PRIDEQ47112.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamPF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSPR01295. CLOACIN.
SMARTSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMSSF69369. SSF69369. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ47112.

Entry information

Entry nameCEA7_ECOLX
AccessionPrimary (citable) accession number: Q47112
Secondary accession number(s): Q51604
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: April 16, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references