Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Colicin-E7

Gene

colE7

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi544Zinc1
Metal bindingi569Zinc1
Metal bindingi573Zinc1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E7 (EC:3.1.-.-)
Gene namesi
Name:colE7
Synonyms:cea
Encoded oniPlasmid ColE70 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002186821 – 576Colicin-E7Add BLAST576

Proteomic databases

PRIDEiQ47112.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
immQ037082EBI-1035016,EBI-1035025

Protein-protein interaction databases

DIPiDIP-16990N.
IntActiQ47112. 1 interactor.
MINTiMINT-95495.

Structurei

Secondary structure

1576
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi89 – 91Combined sources3
Beta strandi97 – 102Combined sources6
Helixi109 – 116Combined sources8
Beta strandi127 – 134Combined sources8
Helixi136 – 142Combined sources7
Beta strandi149 – 155Combined sources7
Helixi156 – 158Combined sources3
Helixi164 – 166Combined sources3
Beta strandi173 – 177Combined sources5
Beta strandi179 – 185Combined sources7
Beta strandi188 – 197Combined sources10
Beta strandi199 – 205Combined sources7
Beta strandi214 – 219Combined sources6
Beta strandi222 – 229Combined sources8
Beta strandi243 – 245Combined sources3
Turni258 – 261Combined sources4
Beta strandi262 – 268Combined sources7
Helixi271 – 273Combined sources3
Beta strandi278 – 284Combined sources7
Helixi288 – 307Combined sources20
Beta strandi447 – 449Combined sources3
Beta strandi451 – 454Combined sources4
Turni462 – 469Combined sources8
Helixi478 – 484Combined sources7
Beta strandi488 – 491Combined sources4
Helixi492 – 504Combined sources13
Helixi507 – 510Combined sources4
Helixi515 – 522Combined sources8
Helixi531 – 533Combined sources3
Beta strandi542 – 547Combined sources6
Helixi549 – 551Combined sources3
Beta strandi555 – 557Combined sources3
Helixi558 – 560Combined sources3
Beta strandi561 – 564Combined sources4
Helixi566 – 572Combined sources7
Turni573 – 575Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M08X-ray2.10A/B447-576[»]
1MZ8X-ray2.00B/D446-576[»]
1PT3X-ray2.50A/B449-576[»]
1UJZX-ray2.10B446-573[»]
1ZNSX-ray2.50A444-576[»]
1ZNVX-ray2.00B/D444-576[»]
2AXCX-ray1.70A60-316[»]
2ERHX-ray2.00B447-573[»]
2IVHX-ray2.80A449-576[»]
2JAZX-ray2.03B/D446-576[»]
2JB0X-ray1.91B446-576[»]
2JBGX-ray2.20B/D446-576[»]
3FBDX-ray2.90A/D445-576[»]
3GJNX-ray2.48B/C446-576[»]
3GKLX-ray2.20A/B446-576[»]
3ZFKX-ray1.70A/B450-573[»]
7CEIX-ray2.30B371-576[»]
ProteinModelPortaliQ47112.
SMRiQ47112.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ47112.

Family & Domainsi

Sequence similaritiesi

Belongs to the colicin/pyosin nuclease family.Curated

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMiSSF69369. SSF69369. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGDGRGHN SGAHNTGGNI NGGPTGLGGN GGASDGSGWS SENNPWGGGS
60 70 80 90 100
GSGVHWGGGS GHGNGGGNSN SGGGSNSSVA APMAFGFPAL AAPGAGTLGI
110 120 130 140 150
SVSGEALSAA IADIFAALKG PFKFSAWGIA LYGILPSEIA KDDPNMMSKI
160 170 180 190 200
VTSLPAETVT NVQVSTLPLD QATVSVTKRV TDVVKDTRQH IAVVAGVPMS
210 220 230 240 250
VPVVNAKPTR TPGVFHASFP GVPSLTVSTV KGLPVSTTLP RGITEDKGRT
260 270 280 290 300
AVPAGFTFGG GSHEAVIRFP KESGQKPVYV SVTDVLTPAQ VKQRQDEEKR
310 320 330 340 350
LQQEWNDAHP VEVAERNYEQ ARAELNQANK DVARNQERQA KAVQVYNSRK
360 370 380 390 400
SELDAANKTL ADAKAEIKQF ERFAREPMAA GHRMWQMAGL KAQRAQTDVN
410 420 430 440 450
NKKAAFDAAA KEKSDADVAL SSALERRKQK ENKEKDAKAK LDKESKRNKP
460 470 480 490 500
GKATGKGKPV NNKWLNNAGK DLGSPVPDRI ANKLRDKEFK SFDDFRKKFW
510 520 530 540 550
EEVSKDPELS KQFSRNNNDR MKVGKAPKTR TQDVSGKRTS FELHHEKPIS
560 570
QNGGVYDMDN ISVVTPKRHI DIHRGK
Length:576
Mass (Da):61,350
Last modified:May 30, 2000 - v2
Checksum:iE5B05E73B2E17249
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62409 Genomic DNA. Translation: AAA98054.1.
X63620 Genomic DNA. Translation: CAA45164.1.
PIRiS22453.
RefSeqiWP_021530049.1. NZ_JSJZ01000029.1.
YP_009060493.1. NC_024962.1.

Genome annotation databases

GeneIDi20467021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62409 Genomic DNA. Translation: AAA98054.1.
X63620 Genomic DNA. Translation: CAA45164.1.
PIRiS22453.
RefSeqiWP_021530049.1. NZ_JSJZ01000029.1.
YP_009060493.1. NC_024962.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M08X-ray2.10A/B447-576[»]
1MZ8X-ray2.00B/D446-576[»]
1PT3X-ray2.50A/B449-576[»]
1UJZX-ray2.10B446-573[»]
1ZNSX-ray2.50A444-576[»]
1ZNVX-ray2.00B/D444-576[»]
2AXCX-ray1.70A60-316[»]
2ERHX-ray2.00B447-573[»]
2IVHX-ray2.80A449-576[»]
2JAZX-ray2.03B/D446-576[»]
2JB0X-ray1.91B446-576[»]
2JBGX-ray2.20B/D446-576[»]
3FBDX-ray2.90A/D445-576[»]
3GJNX-ray2.48B/C446-576[»]
3GKLX-ray2.20A/B446-576[»]
3ZFKX-ray1.70A/B450-573[»]
7CEIX-ray2.30B371-576[»]
ProteinModelPortaliQ47112.
SMRiQ47112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-16990N.
IntActiQ47112. 1 interactor.
MINTiMINT-95495.

Proteomic databases

PRIDEiQ47112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20467021.

Miscellaneous databases

EvolutionaryTraceiQ47112.

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMiSSF69369. SSF69369. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCEA7_ECOLX
AccessioniPrimary (citable) accession number: Q47112
Secondary accession number(s): Q51604
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 30, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.