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Protein

Colicin-E7

Gene

colE7

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi544 – 5441Zinc
Metal bindingi569 – 5691Zinc
Metal bindingi573 – 5731Zinc

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cytolysis Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E7 (EC:3.1.-.-)
Gene namesi
Name:colE7
Synonyms:cea
Encoded oniPlasmid ColE70 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  1. extrachromosomal circular DNA Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Colicin-E7PRO_0000218682Add
BLAST

Proteomic databases

PRIDEiQ47112.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
immQ037082EBI-1035016,EBI-1035025

Protein-protein interaction databases

DIPiDIP-16990N.
IntActiQ47112. 1 interaction.
MINTiMINT-95495.

Structurei

Secondary structure

1
576
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi89 – 913Combined sources
Beta strandi97 – 1026Combined sources
Helixi109 – 1168Combined sources
Beta strandi127 – 1348Combined sources
Helixi136 – 1427Combined sources
Beta strandi149 – 1557Combined sources
Helixi156 – 1583Combined sources
Helixi164 – 1663Combined sources
Beta strandi173 – 1775Combined sources
Beta strandi179 – 1857Combined sources
Beta strandi188 – 19710Combined sources
Beta strandi199 – 2057Combined sources
Beta strandi214 – 2196Combined sources
Beta strandi222 – 2298Combined sources
Beta strandi243 – 2453Combined sources
Turni258 – 2614Combined sources
Beta strandi262 – 2687Combined sources
Helixi271 – 2733Combined sources
Beta strandi278 – 2847Combined sources
Helixi288 – 30720Combined sources
Beta strandi447 – 4493Combined sources
Beta strandi451 – 4544Combined sources
Turni462 – 4698Combined sources
Helixi478 – 4847Combined sources
Beta strandi488 – 4914Combined sources
Helixi492 – 50413Combined sources
Helixi507 – 5104Combined sources
Helixi515 – 5228Combined sources
Helixi531 – 5333Combined sources
Beta strandi542 – 5476Combined sources
Helixi549 – 5513Combined sources
Beta strandi555 – 5573Combined sources
Helixi558 – 5603Combined sources
Beta strandi561 – 5644Combined sources
Helixi566 – 5727Combined sources
Turni573 – 5753Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M08X-ray2.10A/B447-576[»]
1MZ8X-ray2.00B/D446-576[»]
1PT3X-ray2.50A/B449-576[»]
1UJZX-ray2.10B446-573[»]
1ZNSX-ray2.50A444-576[»]
1ZNVX-ray2.00B/D444-576[»]
2AXCX-ray1.70A60-316[»]
2ERHX-ray2.00B447-573[»]
2IVHX-ray2.80A449-576[»]
2JAZX-ray2.03B/D446-576[»]
2JB0X-ray1.91B446-576[»]
2JBGX-ray2.20B/D446-576[»]
3FBDX-ray2.90A/D445-576[»]
3GJNX-ray2.48B/C446-576[»]
3GKLX-ray2.20A/B446-576[»]
3ZFKX-ray1.70A/B450-573[»]
7CEIX-ray2.30B371-576[»]
ProteinModelPortaliQ47112.
SMRiQ47112. Positions 77-576.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ47112.

Family & Domainsi

Sequence similaritiesi

Belongs to the colicin/pyosin nuclease family.Curated

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMiSSF69369. SSF69369. 1 hit.

Sequencei

Sequence statusi: Complete.

Q47112-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGGDGRGHN SGAHNTGGNI NGGPTGLGGN GGASDGSGWS SENNPWGGGS
60 70 80 90 100
GSGVHWGGGS GHGNGGGNSN SGGGSNSSVA APMAFGFPAL AAPGAGTLGI
110 120 130 140 150
SVSGEALSAA IADIFAALKG PFKFSAWGIA LYGILPSEIA KDDPNMMSKI
160 170 180 190 200
VTSLPAETVT NVQVSTLPLD QATVSVTKRV TDVVKDTRQH IAVVAGVPMS
210 220 230 240 250
VPVVNAKPTR TPGVFHASFP GVPSLTVSTV KGLPVSTTLP RGITEDKGRT
260 270 280 290 300
AVPAGFTFGG GSHEAVIRFP KESGQKPVYV SVTDVLTPAQ VKQRQDEEKR
310 320 330 340 350
LQQEWNDAHP VEVAERNYEQ ARAELNQANK DVARNQERQA KAVQVYNSRK
360 370 380 390 400
SELDAANKTL ADAKAEIKQF ERFAREPMAA GHRMWQMAGL KAQRAQTDVN
410 420 430 440 450
NKKAAFDAAA KEKSDADVAL SSALERRKQK ENKEKDAKAK LDKESKRNKP
460 470 480 490 500
GKATGKGKPV NNKWLNNAGK DLGSPVPDRI ANKLRDKEFK SFDDFRKKFW
510 520 530 540 550
EEVSKDPELS KQFSRNNNDR MKVGKAPKTR TQDVSGKRTS FELHHEKPIS
560 570
QNGGVYDMDN ISVVTPKRHI DIHRGK
Length:576
Mass (Da):61,350
Last modified:May 30, 2000 - v2
Checksum:iE5B05E73B2E17249
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62409 Genomic DNA. Translation: AAA98054.1.
X63620 Genomic DNA. Translation: CAA45164.1.
PIRiS22453.
RefSeqiYP_009060493.1. NC_024962.1.

Genome annotation databases

GeneIDi20467021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62409 Genomic DNA. Translation: AAA98054.1.
X63620 Genomic DNA. Translation: CAA45164.1.
PIRiS22453.
RefSeqiYP_009060493.1. NC_024962.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M08X-ray2.10A/B447-576[»]
1MZ8X-ray2.00B/D446-576[»]
1PT3X-ray2.50A/B449-576[»]
1UJZX-ray2.10B446-573[»]
1ZNSX-ray2.50A444-576[»]
1ZNVX-ray2.00B/D444-576[»]
2AXCX-ray1.70A60-316[»]
2ERHX-ray2.00B447-573[»]
2IVHX-ray2.80A449-576[»]
2JAZX-ray2.03B/D446-576[»]
2JB0X-ray1.91B446-576[»]
2JBGX-ray2.20B/D446-576[»]
3FBDX-ray2.90A/D445-576[»]
3GJNX-ray2.48B/C446-576[»]
3GKLX-ray2.20A/B446-576[»]
3ZFKX-ray1.70A/B450-573[»]
7CEIX-ray2.30B371-576[»]
ProteinModelPortaliQ47112.
SMRiQ47112. Positions 77-576.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-16990N.
IntActiQ47112. 1 interaction.
MINTiMINT-95495.

Proteomic databases

PRIDEiQ47112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20467021.

Miscellaneous databases

EvolutionaryTraceiQ47112.

Family and domain databases

Gene3Di1.10.287.620. 1 hit.
3.90.540.10. 1 hit.
InterProiIPR024575. Cloacin_colicin_fam.
IPR024622. Colicin/pyocin_DNase_dom.
IPR024566. Colicin_R_dom.
IPR003615. HNH_nuc.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF12639. Colicin-DNase. 1 hit.
PF11570. E2R135. 1 hit.
[Graphical view]
PRINTSiPR01295. CLOACIN.
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
SUPFAMiSSF69369. SSF69369. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the cea gene of the ColE7 plasmid."
    Soong B.W., Lu F.M., Chak K.-F.
    Mol. Gen. Genet. 233:177-183(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K317.
  2. "Nucleotide sequence encoding the immunity and lysis proteins and the carboxyl-terminal peptides of colicins E4 and E7."
    Lau P.C.K., Parsons M.
    Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 371-576.
    Strain: K317.
  3. "The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein."
    Ko T.P., Liao C.C., Ku W.-Y., Chak K.-F., Yuan H.S.
    Structure 7:91-102(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 371-576.

Entry informationi

Entry nameiCEA7_ECOLX
AccessioniPrimary (citable) accession number: Q47112
Secondary accession number(s): Q51604
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: January 7, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.