Q470S7 (KATG2_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 2
Short name=CP 2
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	Reut_A1741

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000354877
Sites
	Active site	92	1	Proton acceptor By similarity
	Metal binding	255	1	Iron (heme axial ligand) By similarity
	Site	88	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	91 ↔ 214		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-240) By similarity
	Cross-link	214 ↔ 240		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q470S7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 5372D3204B187B5E	FASTA	728	79,863
	10 20 30 40 50 60 MSTEAKCPFN HAAGSGTSNR DWWPHQLNLG ILRQHSSLAD PMDKGFNYAE EFKSLDLAAV 70 80 90 100 110 120 KHDLAALMTV SQDWWPADFG HYGGLFIRMA WHAAGTYRTG DGRGGAGSGQ QRFAPLNSWP 130 140 150 160 170 180 DNVNLDKARR LLWPIKQKYG KKISWADLIV LTGNVALESM GFKTFGFGGG REDVWEPDQD 190 200 210 220 230 240 VYWGSETTWL ADKRYSGVRD LENPLAAVQM GLIYVNPEGP NGNPDPAKAA VDIRETFARM 250 260 270 280 290 300 AMNDEETVAL IAGGHTFGKT HGAGPASHVG PEPEAAGIEE QGLGWRSSFG SGKGSDAITS 310 320 330 340 350 360 GLEVIWTSTP TKWSNNFFWN LFGYEWELTK SPAGAHQWKP NGDAGANSIP DPYDPSRRRG 370 380 390 400 410 420 PTMLTTDLSL RVDPAYEKIS RRFHENPDQF ADAFARAWFK LTHRDMGPRS RYLGPEVPAE 430 440 450 460 470 480 ELIWQDPIPA VDHVLIDEQD VAALKGKILA TGVPVSQLVS TAWASASTFR GSDKRGGANG 490 500 510 520 530 540 ARIRLAPQKD WEVNQPAVLA QVLETLEGIR NEFNSSQSGG KKVSLADLIV LAGCAGIEQA 550 560 570 580 590 600 AKKGGHDVKV PFTPGRMDAT QEQTDVESFS VMEPIADGFR NYLKGTYAVT AETLLVDKAQ 610 620 630 640 650 660 LLTLTVPEVT ALVGGMRVLN ANFGQTQHGV FTDRPETLTN DFFVNVLDMG TEWKPTADNK 670 680 690 700 710 720 DLFEGYDRAT GKLKWTGTRV DLIFGSNSEL RAVAEVYGCA DAKEKFVQDF VAAWNKVMNL DRFEAANR

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ61106.1.
RefSeq	YP_295950.1. NC_007347.1.
3D structure databases
HSSP	HSSP built from PDB template 2CCA based on UniProtKB Q50555.
ProteinModelPortal	Q470S7.
SMR	Q470S7. Positions 14-726.
ModBase	Search...
Protein-protein interaction databases
STRING	Q470S7.
Protein family/group databases
PeroxiBase	2322. ReCP01_JMP134.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3609473.
GenomeReviews	Gene locus Reut_A1741 in contig CP000090_GR.
KEGG	reu:Reut_A1741.
NMPDR	fig|264198.3.peg.2368.
PATRIC	20229015. VBIRalEut24049_2370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_A1741-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_CUPPJ

Accession

Primary (citable) accession number: Q470S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program