Q470E9 (LPXA_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Short name=UDP-N-acetylglucosamine acyltransferase
EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	Reut_A1869

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus pinatubonensis

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00387
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine. HAMAP-Rule MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP-Rule MF_00387
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP-Rule MF_00387		PRO_0000302596

Sequences

	Sequence		Length	Mass (Da)
	Q470E9 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 5D710F04787FFD15	FASTA	267	28,199
	10 20 30 40 50 60 MTQIHPTALV DPKAELAADV TVGPFSIVGP NVRIGSGTSI GAHSTVEGHT TIGQGNNIGP 70 80 90 100 110 120 YASVGGVPQD MKYRNEPTRL DIGDRNTIRE FTTIHTGTVQ DRGVTTIGSD NWIMAYVHIA 130 140 150 160 170 180 HDCTVGNHTV FSSNAQIAGH VEVGDWAILG GMSGVHQFVR IGAHAMLGGA SALVQDVPPF 190 200 210 220 230 240 VIAASDKNGN KATPHGINVE GLRRRGFDAG QIAGLRQAYK LLYKSDLSFD EARNEIAALL 250 260 AQADASAAEP LRAFLDFIAA TQRGIVR

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ61234.1.
RefSeq	YP_296078.1. NC_007347.1.
3D structure databases
ProteinModelPortal	Q470E9.
SMR	Q470E9. Positions 2-267.
ModBase	Search...
Protein-protein interaction databases
STRING	264198.Reut_A1869.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ61234; AAZ61234; Reut_A1869.
GeneID	3609256.
KEGG	reu:Reut_A1869.
PATRIC	20229289. VBIRalEut24049_2503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1043.
HOGENOM	HOG000294326.
KO	K00677.
OMA	REFCTFN.
ProtClustDB	PRK05289.
Enzyme and pathway databases
BioCyc	CPIN264198:GIW3-1889-MONOMER.
UniPathway	UPA00359; UER00477.
Family and domain databases
HAMAP	MF_00387. LpxA.
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR010137. Lipid_A_LpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00132. Hexapep. 5 hits. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01852. lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_CUPPJ

Accession

Primary (citable) accession number: Q470E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program