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Q470D5

- GLND_CUPPJ

UniProt

Q470D5 - GLND_CUPPJ

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-1903-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:Reut_A1883
OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002697: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 869869Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000231689Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_A1883.

Structurei

3D structure databases

ProteinModelPortaliQ470D5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini451 – 54898HDUniRule annotationAdd
BLAST
Domaini689 – 77385ACT 1UniRule annotationAdd
BLAST
Domaini800 – 86970ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 331331UridylyltransferaseAdd
BLAST
Regioni332 – 688357Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q470D5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTNLPALPM DTTPELLLAA RVRDQLKADK QALFADFDLS SHVGTLVTRL
60 70 80 90 100
RRAVDAALAE AWRGLDMPAD AALVAVGGYG RGELFPYSDV DVLLLLRAEP
110 120 130 140 150
DADTVSRLER FIGMCWDLGL EIGSSVRTVE DCIREARADI TIQTSLLEAR
160 170 180 190 200
LLTGNRKLFE ALRTQHQADL DPAAFFQAKL LEMRQRHAKY QDTPYALEPN
210 220 230 240 250
CKESPGGLRD LQVILWMTKA AGLGDSWKEL FERGLLTQRE AQELSRNERL
260 270 280 290 300
LKTIRARLHL VAGRRQDVLV FDLQTALAES FGYRQNANKR ASEQLMRRYF
310 320 330 340 350
WAAKAVTQLN SVLLLNIEAL LFPSESQVTR VINERFVERQ GMLEITSDSL
360 370 380 390 400
YEDDPHAILE TFLLYERTPG IKGLSPRTLR GLYNARTVMD ARWRSDPENR
410 420 430 440 450
RLFLAILQEP QGITHALRLM NQTSVLGRYL INFRRIVGQM QHDLFHVYTV
460 470 480 490 500
DQHILMVVRN MRRFAIVEHT HEFPFCSQLM ASFDKPWVLS VAALFHDIAK
510 520 530 540 550
GRGGDHSKLG TVDARRFCKQ HGIGREDADL ICWLVEHHLT MSHVAQKQDL
560 570 580 590 600
TDPDVVHAFA RVVGDERHLT ALYLLTVADV RGTSPKVWNA WKGKLLEDLY
610 620 630 640 650
RITLRVLGGA RVDPHSIWAQ RQEETISQLR LKAFDPELGK PLWAQLDVAF
660 670 680 690 700
FLRHDSRDIA WLTRHLFNKV DSPVPVVKAR ISPAGEGLQV AVYVKDQPDL
710 720 730 740 750
FARICGYFER KAFSIQDAKI HTTRHGYALD TFQVTDPGLA DDGGNYRDIL
760 770 780 790 800
ALVEHELGDR LQQQAALPEP SQGRLSRQSR SFPIKPRVDL RPDERGQYYL
810 820 830 840 850
LSLSANDRTG LLYAITRVLA KHRVSVHTAR INTLGERVED VFLVDGSRLA
860
ADNRLQIQLE QDLLAALEI
Length:869
Mass (Da):98,722
Last modified:September 13, 2005 - v1
Checksum:i8028D86E94BE91AE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000090 Genomic DNA. Translation: AAZ61248.1.
RefSeqiYP_296092.1. NC_007347.1.

Genome annotation databases

EnsemblBacteriaiAAZ61248; AAZ61248; Reut_A1883.
GeneIDi3609562.
KEGGireu:Reut_A1883.
PATRICi20229317. VBIRalEut24049_2517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000090 Genomic DNA. Translation: AAZ61248.1 .
RefSeqi YP_296092.1. NC_007347.1.

3D structure databases

ProteinModelPortali Q470D5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264198.Reut_A1883.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ61248 ; AAZ61248 ; Reut_A1883 .
GeneIDi 3609562.
KEGGi reu:Reut_A1883.
PATRICi 20229317. VBIRalEut24049_2517.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci CPIN264198:GIW3-1903-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JMP134 / LMG 1197.

Entry informationi

Entry nameiGLND_CUPPJ
AccessioniPrimary (citable) accession number: Q470D5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3