Q47096 (GUNV_PECCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 5
EC=3.2.1.4

Alternative name(s):
Cellulase V
Endo-1,4-beta-glucanase V
Endoglucanase V

Gene names

Name:

celV

Organism

Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)

Taxonomic identifier

555 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium › Erwinia carotovora

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endoglucanase with some exoglucanase activity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM3 (carbohydrate binding type-3) domain.
Biophysicochemical properties	pH dependence: Optimum pH is about 7.0. Temperature dependence: Optimum temperature is about 42 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 505	474	Endoglucanase 5		PRO_0000007858
Regions
	Domain	353 – 505	153	CBM3
	Region	32 – 334	303	Catalytic
	Region	335 – 352	18	Linker
Sites
	Active site	168	1	Proton donor By similarity
	Active site	256	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q47096 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DBEA9337BB4D2623	FASTA	505	54,900
	10 20 30 40 50 60 MWMRRNQIVR KLTLGVVTTV LGMSLSFSAL SATPVETHGQ LSIENGRLVD EQGKRVQLRG 70 80 90 100 110 120 ISSHGLQWFG DYVNKDSMKW LRDDWGINVF RVAMYTAADG YISNPSLANK VKEAVAAAQS 130 140 150 160 170 180 LGVYIIIDWH ILSDNDPNIY KAQAKTFFAE MAGLYGSSPN VIYEIANEPN GGVTWNGQIR 190 200 210 220 230 240 PYALEVTDTI RSKDPDNLII VGTGTWSQDI HDAADNQLPD PNTMYALHFY AGTHGQFLRD 250 260 270 280 290 300 RIDYAQSRGA AIFVSEWGTS DASGNGGPFL PESQTWIDFL NNRGVSWVNW SLTDKSEASA 310 320 330 340 350 360 ALAPGASKSG GWTEQNLSTS GKFVREQIRA GANLGGGDTP TTPTEPTNPG NGTTGDVVLQ 370 380 390 400 410 420 YRNVDNNPSD DAIRMAVNIK NTGSTPIKLS DLQVRYYFHD DGKPGANLFV DWANVGPNNI 430 440 450 460 470 480 VTSTGTPAAS TDKANRYVLV TFSSGAGSLQ PGAETGEVQV RIHAGDWSNV NETNDYSYGA 490 500 NVTSYANWDK ITVHDKGTLV WGVEP

References

[1]	"Molecular analysis of the major cellulase (CelV) of Erwinia carotovora: evidence for an evolutionary 'mix-and-match' of enzyme domains." Cooper V.J.C., Salmond G.P.C. Mol. Gen. Genet. 241:341-350(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SCRI 193.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76000 Genomic DNA. Translation: CAA53592.1.
PIR	S39962.
3D structure databases
ProteinModelPortal	Q47096.
SMR	Q47096. Positions 35-333.
ModBase	Search...
Protein family/group databases
CAZy	CBM3. Carbohydrate-Binding Module Family 3. GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.40.710. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR008965. Carb-bd_dom. IPR001956. CBD_3. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00942. CBM_3. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view]
SMART	SM01067. CBM_3. 1 hit. [Graphical view]
SUPFAM	SSF49384. Cellul_bind. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS51172. CBM3. 1 hit. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNV_PECCC

Accession

Primary (citable) accession number: Q47096

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program