SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q47077

- BGAL1_ENTCL

UniProt

Q47077 - BGAL1_ENTCL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Beta-galactosidase
Gene
lacZ
Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041Substrate By similarity
Metal bindingi203 – 2031Sodium By similarity
Binding sitei203 – 2031Substrate By similarity
Sitei359 – 3591Transition state stabilizer By similarity
Sitei393 – 3931Transition state stabilizer By similarity
Metal bindingi418 – 4181Magnesium 1 By similarity
Metal bindingi420 – 4201Magnesium 1 By similarity
Active sitei463 – 4631Proton donor By similarity
Metal bindingi463 – 4631Magnesium 1 By similarity
Binding sitei463 – 4631Substrate By similarity
Active sitei539 – 5391Nucleophile By similarity
Metal bindingi599 – 5991Magnesium 2 By similarity
Metal bindingi603 – 6031Sodium; via carbonyl oxygen By similarity
Metal bindingi606 – 6061Sodium By similarity
Binding sitei606 – 6061Substrate By similarity
Binding sitei1003 – 10031Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10281028Beta-galactosidaseUniRule annotation
PRO_0000057661Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ47077.
SMRiQ47077. Positions 15-1026.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni539 – 5424Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47077-1 [UniParc]FASTAAdd to Basket

« Hide

MSDTPSLTLS ALLARRDWEN PVVTQWNRLA AHAPLHSWRN EPSARDDAGS     50
ARRQTLNGLW RFSYFTAPEQ VPQAWVTEDC ADAVAMPVPS NWQMQGFDTP 100
IYTNVTYPIP VNPPFVPQEN PTGCYSLTFE VDDAWLQSGQ TRIIFDGVNS 150
AFHLWCNGQW IGYSQDSRLP AEFDLSAALR PGQNRLAVMV LRWCDGSYLE 200
DQDMWRMSGI FRDVTLLHKP ETQIADYHVV TDLNAELDRA VLKVDVTLAG 250
AGFADGEVVF TLWRKGEKCA SVSRQPGSAI VDERGSWAER LTVTIPVETP 300
ALWSAETPEL YRLTMALLNP QGEVLEIEAC DVGFRRVEIS NGLLKLNGKP 350
LLIRGVNRHE HHSENGQVMD EATMRRDIET MKQHSFNAVR CSHYPNHPLW 400
YQLCDRYGLY VVDEANIETH GMVPMSRLAD DPRWLPAMSE RVTRMVQRDR 450
NHPSIIIWSL GNESGHGANH DALYRWLKTT DPTRPVQYEG GGANTAATDI 500
VCPMYARVDR DQPFPAVPKW SIKKWIGMPD ETRPLILCEY AHAMGNSFGG 550
FAKYWQAFRS HPRLQGGFVW DWVDQALTKR DEDGNTFWAY GGDFGDKPND 600
RQFCLNGLVF PDRTPHPALY EAHGPQQFFT FTRVSTSPLV IEVQSGYLFR 650
HTDNEVLNWT VARDGDVLVA GEVTLAMVPE GTQRLEIALP ELNAGPGEVW 700
LNVEVRQPRA TPWSPAAIAA AGSSGRFRLR SLLLRQPRRR AAVLTQTDRI 750
LEIAHRQQRW QFDRASGNLT QWWRNGVETL LSPLTDNVSR APLDNDIGVS 800
EATKIDPNAW VERWKAAGMY DLTPRVLHCE AEQHAGEVVV TTQHVLEYRG 850
KALFLSRKVW RIDEQGVLHG DIQVDMASDI PEPARIGLSV HLAETPENVR 900
WLGLGPHENY PDRKLAAQQG RWTLPLEAMH TPYIFPTENG LRCDTRELVV 950
GMHQLNGHFH FSVSRYSQQQ LRETTHHHLL REEPGCWLNL DAFHMGVGGD 1000
DSWSPSVSPE FILQTRQLRY TFSWQQNP 1028
Length:1,028
Mass (Da):116,678
Last modified:November 1, 1996 - v1
Checksum:iF4D61F55F9FEEAEC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D42077 Genomic DNA. Translation: BAA07673.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D42077 Genomic DNA. Translation: BAA07673.1 .

3D structure databases

ProteinModelPortali Q47077.
SMRi Q47077. Positions 15-1026.
ModBasei Search...

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of the beta-galactosidase gene from Enterobacter cloacae GAO."
    Nagano H., Kawaguchi T., Omori M., Shoji Z., Arai M.
    Biosci. Biotechnol. Biochem. 58:1866-1869(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GAO.

Entry informationi

Entry nameiBGAL1_ENTCL
AccessioniPrimary (citable) accession number: Q47077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi