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Protein

Beta-galactosidase

Gene

lacZ

Organism
Enterobacter cloacae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041SubstrateUniRule annotation
Metal bindingi203 – 2031SodiumUniRule annotation
Binding sitei203 – 2031SubstrateUniRule annotation
Sitei359 – 3591Transition state stabilizerUniRule annotation
Sitei393 – 3931Transition state stabilizerUniRule annotation
Metal bindingi418 – 4181Magnesium 1UniRule annotation
Metal bindingi420 – 4201Magnesium 1UniRule annotation
Active sitei463 – 4631Proton donorUniRule annotation
Metal bindingi463 – 4631Magnesium 1UniRule annotation
Binding sitei463 – 4631SubstrateUniRule annotation
Active sitei539 – 5391NucleophileUniRule annotation
Metal bindingi599 – 5991Magnesium 2UniRule annotation
Metal bindingi603 – 6031Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi606 – 6061SodiumUniRule annotation
Binding sitei606 – 6061SubstrateUniRule annotation
Binding sitei1003 – 10031SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10281028Beta-galactosidasePRO_0000057661Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ47077.
SMRiQ47077. Positions 15-1026.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni539 – 5424Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47077-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDTPSLTLS ALLARRDWEN PVVTQWNRLA AHAPLHSWRN EPSARDDAGS
60 70 80 90 100
ARRQTLNGLW RFSYFTAPEQ VPQAWVTEDC ADAVAMPVPS NWQMQGFDTP
110 120 130 140 150
IYTNVTYPIP VNPPFVPQEN PTGCYSLTFE VDDAWLQSGQ TRIIFDGVNS
160 170 180 190 200
AFHLWCNGQW IGYSQDSRLP AEFDLSAALR PGQNRLAVMV LRWCDGSYLE
210 220 230 240 250
DQDMWRMSGI FRDVTLLHKP ETQIADYHVV TDLNAELDRA VLKVDVTLAG
260 270 280 290 300
AGFADGEVVF TLWRKGEKCA SVSRQPGSAI VDERGSWAER LTVTIPVETP
310 320 330 340 350
ALWSAETPEL YRLTMALLNP QGEVLEIEAC DVGFRRVEIS NGLLKLNGKP
360 370 380 390 400
LLIRGVNRHE HHSENGQVMD EATMRRDIET MKQHSFNAVR CSHYPNHPLW
410 420 430 440 450
YQLCDRYGLY VVDEANIETH GMVPMSRLAD DPRWLPAMSE RVTRMVQRDR
460 470 480 490 500
NHPSIIIWSL GNESGHGANH DALYRWLKTT DPTRPVQYEG GGANTAATDI
510 520 530 540 550
VCPMYARVDR DQPFPAVPKW SIKKWIGMPD ETRPLILCEY AHAMGNSFGG
560 570 580 590 600
FAKYWQAFRS HPRLQGGFVW DWVDQALTKR DEDGNTFWAY GGDFGDKPND
610 620 630 640 650
RQFCLNGLVF PDRTPHPALY EAHGPQQFFT FTRVSTSPLV IEVQSGYLFR
660 670 680 690 700
HTDNEVLNWT VARDGDVLVA GEVTLAMVPE GTQRLEIALP ELNAGPGEVW
710 720 730 740 750
LNVEVRQPRA TPWSPAAIAA AGSSGRFRLR SLLLRQPRRR AAVLTQTDRI
760 770 780 790 800
LEIAHRQQRW QFDRASGNLT QWWRNGVETL LSPLTDNVSR APLDNDIGVS
810 820 830 840 850
EATKIDPNAW VERWKAAGMY DLTPRVLHCE AEQHAGEVVV TTQHVLEYRG
860 870 880 890 900
KALFLSRKVW RIDEQGVLHG DIQVDMASDI PEPARIGLSV HLAETPENVR
910 920 930 940 950
WLGLGPHENY PDRKLAAQQG RWTLPLEAMH TPYIFPTENG LRCDTRELVV
960 970 980 990 1000
GMHQLNGHFH FSVSRYSQQQ LRETTHHHLL REEPGCWLNL DAFHMGVGGD
1010 1020
DSWSPSVSPE FILQTRQLRY TFSWQQNP
Length:1,028
Mass (Da):116,678
Last modified:November 1, 1996 - v1
Checksum:iF4D61F55F9FEEAEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42077 Genomic DNA. Translation: BAA07673.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42077 Genomic DNA. Translation: BAA07673.1.

3D structure databases

ProteinModelPortaliQ47077.
SMRiQ47077. Positions 15-1026.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of the beta-galactosidase gene from Enterobacter cloacae GAO."
    Nagano H., Kawaguchi T., Omori M., Shoji Z., Arai M.
    Biosci. Biotechnol. Biochem. 58:1866-1869(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GAO.

Entry informationi

Entry nameiBGAL1_ENTCL
AccessioniPrimary (citable) accession number: Q47077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.