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Q47077

- BGAL1_ENTCL

UniProt

Q47077 - BGAL1_ENTCL

Protein

Beta-galactosidase

Gene

lacZ

Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041SubstrateUniRule annotation
    Metal bindingi203 – 2031SodiumUniRule annotation
    Binding sitei203 – 2031SubstrateUniRule annotation
    Sitei359 – 3591Transition state stabilizerUniRule annotation
    Sitei393 – 3931Transition state stabilizerUniRule annotation
    Metal bindingi418 – 4181Magnesium 1UniRule annotation
    Metal bindingi420 – 4201Magnesium 1UniRule annotation
    Active sitei463 – 4631Proton donorUniRule annotation
    Metal bindingi463 – 4631Magnesium 1UniRule annotation
    Binding sitei463 – 4631SubstrateUniRule annotation
    Active sitei539 – 5391NucleophileUniRule annotation
    Metal bindingi599 – 5991Magnesium 2UniRule annotation
    Metal bindingi603 – 6031Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi606 – 6061SodiumUniRule annotation
    Binding sitei606 – 6061SubstrateUniRule annotation
    Binding sitei1003 – 10031SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    OrganismiEnterobacter cloacae
    Taxonomic identifieri550 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10281028Beta-galactosidasePRO_0000057661Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ47077.
    SMRiQ47077. Positions 15-1026.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni539 – 5424Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q47077-1 [UniParc]FASTAAdd to Basket

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    MSDTPSLTLS ALLARRDWEN PVVTQWNRLA AHAPLHSWRN EPSARDDAGS     50
    ARRQTLNGLW RFSYFTAPEQ VPQAWVTEDC ADAVAMPVPS NWQMQGFDTP 100
    IYTNVTYPIP VNPPFVPQEN PTGCYSLTFE VDDAWLQSGQ TRIIFDGVNS 150
    AFHLWCNGQW IGYSQDSRLP AEFDLSAALR PGQNRLAVMV LRWCDGSYLE 200
    DQDMWRMSGI FRDVTLLHKP ETQIADYHVV TDLNAELDRA VLKVDVTLAG 250
    AGFADGEVVF TLWRKGEKCA SVSRQPGSAI VDERGSWAER LTVTIPVETP 300
    ALWSAETPEL YRLTMALLNP QGEVLEIEAC DVGFRRVEIS NGLLKLNGKP 350
    LLIRGVNRHE HHSENGQVMD EATMRRDIET MKQHSFNAVR CSHYPNHPLW 400
    YQLCDRYGLY VVDEANIETH GMVPMSRLAD DPRWLPAMSE RVTRMVQRDR 450
    NHPSIIIWSL GNESGHGANH DALYRWLKTT DPTRPVQYEG GGANTAATDI 500
    VCPMYARVDR DQPFPAVPKW SIKKWIGMPD ETRPLILCEY AHAMGNSFGG 550
    FAKYWQAFRS HPRLQGGFVW DWVDQALTKR DEDGNTFWAY GGDFGDKPND 600
    RQFCLNGLVF PDRTPHPALY EAHGPQQFFT FTRVSTSPLV IEVQSGYLFR 650
    HTDNEVLNWT VARDGDVLVA GEVTLAMVPE GTQRLEIALP ELNAGPGEVW 700
    LNVEVRQPRA TPWSPAAIAA AGSSGRFRLR SLLLRQPRRR AAVLTQTDRI 750
    LEIAHRQQRW QFDRASGNLT QWWRNGVETL LSPLTDNVSR APLDNDIGVS 800
    EATKIDPNAW VERWKAAGMY DLTPRVLHCE AEQHAGEVVV TTQHVLEYRG 850
    KALFLSRKVW RIDEQGVLHG DIQVDMASDI PEPARIGLSV HLAETPENVR 900
    WLGLGPHENY PDRKLAAQQG RWTLPLEAMH TPYIFPTENG LRCDTRELVV 950
    GMHQLNGHFH FSVSRYSQQQ LRETTHHHLL REEPGCWLNL DAFHMGVGGD 1000
    DSWSPSVSPE FILQTRQLRY TFSWQQNP 1028
    Length:1,028
    Mass (Da):116,678
    Last modified:November 1, 1996 - v1
    Checksum:iF4D61F55F9FEEAEC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42077 Genomic DNA. Translation: BAA07673.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42077 Genomic DNA. Translation: BAA07673.1 .

    3D structure databases

    ProteinModelPortali Q47077.
    SMRi Q47077. Positions 15-1026.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of the beta-galactosidase gene from Enterobacter cloacae GAO."
      Nagano H., Kawaguchi T., Omori M., Shoji Z., Arai M.
      Biosci. Biotechnol. Biochem. 58:1866-1869(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: GAO.

    Entry informationi

    Entry nameiBGAL1_ENTCL
    AccessioniPrimary (citable) accession number: Q47077
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3