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Q47077 (BGAL1_ENTCL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
OrganismEnterobacter cloacae
Taxonomic identifier550 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Protein attributes

Sequence length1028 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10281028Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000057661

Regions

Region539 – 5424Substrate binding By similarity

Sites

Active site4631Proton donor By similarity
Active site5391Nucleophile By similarity
Metal binding2031Sodium By similarity
Metal binding4181Magnesium 1 By similarity
Metal binding4201Magnesium 1 By similarity
Metal binding4631Magnesium 1 By similarity
Metal binding5991Magnesium 2 By similarity
Metal binding6031Sodium; via carbonyl oxygen By similarity
Metal binding6061Sodium By similarity
Binding site1041Substrate By similarity
Binding site2031Substrate By similarity
Binding site4631Substrate By similarity
Binding site6061Substrate By similarity
Binding site10031Substrate By similarity
Site3591Transition state stabilizer By similarity
Site3931Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q47077 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F4D61F55F9FEEAEC

FASTA1,028116,678
        10         20         30         40         50         60 
MSDTPSLTLS ALLARRDWEN PVVTQWNRLA AHAPLHSWRN EPSARDDAGS ARRQTLNGLW 

        70         80         90        100        110        120 
RFSYFTAPEQ VPQAWVTEDC ADAVAMPVPS NWQMQGFDTP IYTNVTYPIP VNPPFVPQEN 

       130        140        150        160        170        180 
PTGCYSLTFE VDDAWLQSGQ TRIIFDGVNS AFHLWCNGQW IGYSQDSRLP AEFDLSAALR 

       190        200        210        220        230        240 
PGQNRLAVMV LRWCDGSYLE DQDMWRMSGI FRDVTLLHKP ETQIADYHVV TDLNAELDRA 

       250        260        270        280        290        300 
VLKVDVTLAG AGFADGEVVF TLWRKGEKCA SVSRQPGSAI VDERGSWAER LTVTIPVETP 

       310        320        330        340        350        360 
ALWSAETPEL YRLTMALLNP QGEVLEIEAC DVGFRRVEIS NGLLKLNGKP LLIRGVNRHE 

       370        380        390        400        410        420 
HHSENGQVMD EATMRRDIET MKQHSFNAVR CSHYPNHPLW YQLCDRYGLY VVDEANIETH 

       430        440        450        460        470        480 
GMVPMSRLAD DPRWLPAMSE RVTRMVQRDR NHPSIIIWSL GNESGHGANH DALYRWLKTT 

       490        500        510        520        530        540 
DPTRPVQYEG GGANTAATDI VCPMYARVDR DQPFPAVPKW SIKKWIGMPD ETRPLILCEY 

       550        560        570        580        590        600 
AHAMGNSFGG FAKYWQAFRS HPRLQGGFVW DWVDQALTKR DEDGNTFWAY GGDFGDKPND 

       610        620        630        640        650        660 
RQFCLNGLVF PDRTPHPALY EAHGPQQFFT FTRVSTSPLV IEVQSGYLFR HTDNEVLNWT 

       670        680        690        700        710        720 
VARDGDVLVA GEVTLAMVPE GTQRLEIALP ELNAGPGEVW LNVEVRQPRA TPWSPAAIAA 

       730        740        750        760        770        780 
AGSSGRFRLR SLLLRQPRRR AAVLTQTDRI LEIAHRQQRW QFDRASGNLT QWWRNGVETL 

       790        800        810        820        830        840 
LSPLTDNVSR APLDNDIGVS EATKIDPNAW VERWKAAGMY DLTPRVLHCE AEQHAGEVVV 

       850        860        870        880        890        900 
TTQHVLEYRG KALFLSRKVW RIDEQGVLHG DIQVDMASDI PEPARIGLSV HLAETPENVR 

       910        920        930        940        950        960 
WLGLGPHENY PDRKLAAQQG RWTLPLEAMH TPYIFPTENG LRCDTRELVV GMHQLNGHFH 

       970        980        990       1000       1010       1020 
FSVSRYSQQQ LRETTHHHLL REEPGCWLNL DAFHMGVGGD DSWSPSVSPE FILQTRQLRY 


TFSWQQNP 

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References

[1]"Molecular cloning and nucleotide sequence of the beta-galactosidase gene from Enterobacter cloacae GAO."
Nagano H., Kawaguchi T., Omori M., Shoji Z., Arai M.
Biosci. Biotechnol. Biochem. 58:1866-1869(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: GAO.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D42077 Genomic DNA. Translation: BAA07673.1.

3D structure databases

ProteinModelPortalQ47077.
SMRQ47077. Positions 15-1026.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL1_ENTCL
AccessionPrimary (citable) accession number: Q47077
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries