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Q47066

- BLT1_ECOLX

UniProt

Q47066 - BLT1_ECOLX

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Protein
Beta-lactamase Toho-1
Gene
bla
Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Has strong cefotaxime-hydrolyzing activity.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Acyl-ester intermediate

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKQ47066.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase Toho-1 (EC:3.5.2.6)
Gene namesi
Name:bla
Encoded oniPlasmid0 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929
Add
BLAST
Chaini30 – 291262Beta-lactamase Toho-1
PRO_0000016994Add
BLAST

Proteomic databases

PRIDEiQ47066.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 4311
Beta strandi46 – 549
Turni55 – 573
Beta strandi60 – 645
Helixi72 – 754
Helixi76 – 8712
Helixi93 – 953
Beta strandi97 – 993
Helixi102 – 1043
Helixi112 – 1154
Beta strandi118 – 1214
Helixi122 – 13110
Helixi135 – 14511
Helixi148 – 15710
Helixi171 – 1733
Helixi186 – 19813
Beta strandi199 – 2024
Helixi204 – 21512
Turni221 – 2233
Helixi224 – 2274
Beta strandi232 – 24110
Turni242 – 2443
Beta strandi245 – 2539
Beta strandi255 – 2573
Beta strandi260 – 2678
Helixi277 – 28812

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BZAX-ray1.80A30-290[»]
1IYOX-ray1.80A30-291[»]
1IYPX-ray2.00A30-291[»]
1IYQX-ray2.10A30-291[»]
1IYSX-ray1.65A31-291[»]
1WE4X-ray1.70A30-291[»]
2WYXneutron diffraction2.10A33-288[»]
2XQZneutron diffraction2.10A32-291[»]
2XR0X-ray2.20A32-291[»]
2ZQ7X-ray0.94A30-291[»]
2ZQ8X-ray1.03A30-291[»]
2ZQ9X-ray1.07A30-291[»]
2ZQAX-ray0.95A30-291[»]
2ZQCX-ray1.07A30-291[»]
2ZQDX-ray1.19A30-291[»]
4BD0X-ray1.21A31-291[»]
4BD1neutron diffraction2.00A31-291[»]
ProteinModelPortaliQ47066.
SMRiQ47066. Positions 31-291.

Miscellaneous databases

EvolutionaryTraceiQ47066.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni237 – 2393Substrate binding

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q47066-1 [UniParc]FASTAAdd to Basket

« Hide

MMTQSIRRSM LTVMATLPLL FSSATLHAQA NSVQQQLEAL EKSSGGRLGV    50
ALINTADNSQ ILYRADERFA MCSTSKVMAA AAVLKQSESD KHLLNQRVEI 100
KKSDLVNYNP IAEKHVNGTM TLAELGAAAL QYSDNTAMNK LIAHLGGPDK 150
VTAFARSLGD ETFRLDRTEP TLNTAIPGDP RDTTTPLAMA QTLKNLTLGK 200
ALAETQRAQL VTWLKGNTTG SASIRAGLPK SWVVGDKTGS GDYGTTNDIA 250
VIWPENHAPL VLVTYFTQPE QKAERRRDIL AAAAKIVTHG F 291
Length:291
Mass (Da):31,447
Last modified:November 1, 1996 - v1
Checksum:i83FC0CD9CD41E7C0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37830 Genomic DNA. Translation: BAA07082.1.
PIRiJP0074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37830 Genomic DNA. Translation: BAA07082.1 .
PIRi JP0074.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BZA X-ray 1.80 A 30-290 [» ]
1IYO X-ray 1.80 A 30-291 [» ]
1IYP X-ray 2.00 A 30-291 [» ]
1IYQ X-ray 2.10 A 30-291 [» ]
1IYS X-ray 1.65 A 31-291 [» ]
1WE4 X-ray 1.70 A 30-291 [» ]
2WYX neutron diffraction 2.10 A 33-288 [» ]
2XQZ neutron diffraction 2.10 A 32-291 [» ]
2XR0 X-ray 2.20 A 32-291 [» ]
2ZQ7 X-ray 0.94 A 30-291 [» ]
2ZQ8 X-ray 1.03 A 30-291 [» ]
2ZQ9 X-ray 1.07 A 30-291 [» ]
2ZQA X-ray 0.95 A 30-291 [» ]
2ZQC X-ray 1.07 A 30-291 [» ]
2ZQD X-ray 1.19 A 30-291 [» ]
4BD0 X-ray 1.21 A 31-291 [» ]
4BD1 neutron diffraction 2.00 A 31-291 [» ]
ProteinModelPortali Q47066.
SMRi Q47066. Positions 31-291.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1697675.

Proteomic databases

PRIDEi Q47066.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK Q47066.

Miscellaneous databases

EvolutionaryTracei Q47066.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A beta-lactamase isolated from Escherichia coli."
    Ishii Y., Ohno A., Taguchi H., Imajo S., Ishiguro M., Matsuzawa H.
    Antimicrob. Agents Chemother. 39:2269-2275(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: TUH12191.
  2. "Crystal structure of the E166A mutant of extended-spectrum beta-lactamase Toho-1 at 1.8 A resolution."
    Ibuka A., Taguchi A., Ishiguro M., Fushinobu S., Ishii Y., Kamitori S., Okuyama K., Yamaguchi K., Konno M., Matsuzawa H.
    J. Mol. Biol. 285:2079-2087(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANT ALA-169.
    Strain: TUH12191.

Entry informationi

Entry nameiBLT1_ECOLX
AccessioniPrimary (citable) accession number: Q47066
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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