Reviewed, UniProtKB/Swiss-Prot Q47066 (BLT1_ECOLX)

Names and origin

Protein names

Recommended name:
    Beta-lactamase Toho-1
    EC=3.5.2.6

Gene names

Name:

bla

Encoded on

Plasmid

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has strong cefotaxime-hydrolyzing activity.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subunit structure	Monomer.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 29

29

Chain

30 – 291

262

Beta-lactamase Toho-1

PRO_0000016994

Regions

Region

237 – 239

3

Substrate binding

Sites

Active site

73

1

Acyl-ester intermediate

Secondary structure

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291

Helix Strand Turn

Details...

Helix

33 – 44

12

Beta strand

46 – 54

9

Turn

55 – 57

3

Beta strand

60 – 64

5

Helix

72 – 75

4

Helix

76 – 87

12

Helix

93 – 95

3

Beta strand

97 – 100

4

Helix

102 – 104

3

Helix

112 – 115

4

Beta strand

118 – 121

4

Helix

122 – 131

10

Helix

135 – 145

11

Helix

148 – 157

10

Helix

171 – 173

3

Helix

186 – 198

13

Beta strand

199 – 202

4

Helix

204 – 215

12

Turn

221 – 223

3

Helix

224 – 227

4

Beta strand

232 – 241

10

Turn

242 – 244

3

Beta strand

245 – 253

9

Beta strand

260 – 267

8

Helix

277 – 287

11

Sequences

	Sequence		Length	Mass (Da)
	Q47066-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 83FC0CD9CD41E7C0	FASTA	291	31,447
	10 20 30 40 50 60 MMTQSIRRSM LTVMATLPLL FSSATLHAQA NSVQQQLEAL EKSSGGRLGV ALINTADNSQ 70 80 90 100 110 120 ILYRADERFA MCSTSKVMAA AAVLKQSESD KHLLNQRVEI KKSDLVNYNP IAEKHVNGTM 130 140 150 160 170 180 TLAELGAAAL QYSDNTAMNK LIAHLGGPDK VTAFARSLGD ETFRLDRTEP TLNTAIPGDP 190 200 210 220 230 240 RDTTTPLAMA QTLKNLTLGK ALAETQRAQL VTWLKGNTTG SASIRAGLPK SWVVGDKTGS 250 260 270 280 290 GDYGTTNDIA VIWPENHAPL VLVTYFTQPE QKAERRRDIL AAAAKIVTHG F

References

[1]	"Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A beta-lactamase isolated from Escherichia coli." Ishii Y., Ohno A., Taguchi H., Imajo S., Ishiguro M., Matsuzawa H. Antimicrob. Agents Chemother. 39:2269-2275(1995) [PubMed: 8619581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TUH12191.
[2]	"Crystal structure of the E166A mutant of extended-spectrum beta-lactamase Toho-1 at 1.8 A resolution." Ibuka A., Taguchi A., Ishiguro M., Fushinobu S., Ishii Y., Kamitori S., Okuyama K., Yamaguchi K., Konno M., Matsuzawa H. J. Mol. Biol. 285:2079-2087(1999) [PubMed: 9925786] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANT ALA-169. Strain: TUH12191.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D37830 Genomic DNA. Translation: BAA07082.1.

PIR

JP0074.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BZA	X-ray	1.80	A	30-291	[»]
1IYO	X-ray	1.80	A	30-291	[»]
1IYP	X-ray	2.00	A	30-291	[»]
1IYQ	X-ray	2.10	A	30-291	[»]
1IYS	X-ray	1.65	A	31-291	[»]
1WE4	X-ray	1.70	A	31-291	[»]
2ZQ7	X-ray	0.94	A	30-291	[»]
2ZQ8	X-ray	1.03	A	30-291	[»]
2ZQ9	X-ray	1.07	A	30-291	[»]
2ZQA	X-ray	0.95	A	30-291	[»]
2ZQC	X-ray	1.07	A	30-291	[»]
2ZQD	X-ray	1.19	A	30-291	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.5.2.6. 246.

Family and domain databases

InterPro

IPR012338. B-lactamase-typ_transpept_fold.
IPR001466. Beta_lactamase-related.
IPR000871. Beta_lactamase_A/D.
[Graphical view]

Pfam

PF00144. Beta-lactamase. 1 hit.
[Graphical view]

PRINTS

PR00118. BLACTAMASEA.

PROSITE

PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BLT1_ECOLX

Accession

Primary (citable) accession number: Q47066

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)