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Protein

Protease ElaD

Gene

elaD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Protease that can act as an efficient and specific deubiquitinating enzyme in vitro. Does not possess desumoylating and deneddylating activities. The physiological substrate is unknown.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei231 – 2311By similarity
Active sitei313 – 3131NucleophileCurated

GO - Molecular functioni

  • cysteine-type peptidase activity Source: EcoCyc

GO - Biological processi

  • protein deubiquitination Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciEcoCyc:G7176-MONOMER.
ECOL316407:JW5840-MONOMER.
MetaCyc:G7176-MONOMER.

Protein family/group databases

MEROPSiC79.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease ElaD (EC:3.4.22.-)
Alternative name(s):
Deubiquitinase
Deubiquitinating enzyme
Short name:
DUB
Deubiquitinating protease
Gene namesi
Name:elaD
Ordered Locus Names:b2269, JW5840
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14261. elaD.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi313 – 3131C → S: Abolishes deubiquitinating activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Protease ElaDPRO_0000086950Add
BLAST

Proteomic databases

PaxDbiQ47013.

Interactioni

Protein-protein interaction databases

BioGridi4261774. 14 interactions.
STRINGi511145.b2269.

Structurei

3D structure databases

ProteinModelPortaliQ47013.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C79 family.Curated

Phylogenomic databases

eggNOGiENOG4105Z6G. Bacteria.
ENOG410XTR6. LUCA.
HOGENOMiHOG000054843.
KOiK18015.
OMAiYRLSTPQ.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVTVVSNYC QLSQTQLSQT FAEKFTVTEE LLQSLKKTAL SGDEESIELL
60 70 80 90 100
HNIALGYDKF GKEAEDILYH IVRTPTNETL SIIRLIKNAC LKLYNLAHIA
110 120 130 140 150
TNSPLKSHDS DDLLFKKLFS PSKLMTIIGD EIPLISEKQS LSKVLLNDEN
160 170 180 190 200
NELSDGTNFW DKNRQLTTDE IACYLQKIAA NAKNTQVNYP TGLYVPYSTR
210 220 230 240 250
THLEDALNEN IKSDPSWPNE VQLFPINTGG HWILVSLQKI VNKKNNKLQI
260 270 280 290 300
KCVIFNSLRA LGYDKENSLK RVINSFNSEL MGEMSNNNIK VHLNEPEIIF
310 320 330 340 350
LHADLQQYLS QSCGAFVCMA AQEVIEQRES NSDSAPYTLL KNHADRFKKY
360 370 380 390 400
SAEEQYEIDF QHRLANRNCY LDKYGDANIN HYYRNLEIKH SQPKNRASGK

RVS
Length:403
Mass (Da):46,078
Last modified:December 4, 2007 - v3
Checksum:i775AFB6D5AE37DC0
GO

Sequence cautioni

The sequence AAB02733 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161Q → QQ in AAB02733 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58768 Genomic DNA. Translation: AAB02733.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75329.1.
AP009048 Genomic DNA. Translation: BAE76677.1.
PIRiC64998.
RefSeqiNP_416772.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75329; AAC75329; b2269.
BAE76677; BAE76677; BAE76677.
GeneIDi946742.
KEGGiecj:JW5840.
eco:b2269.
PATRICi32119905. VBIEscCol129921_2362.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58768 Genomic DNA. Translation: AAB02733.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75329.1.
AP009048 Genomic DNA. Translation: BAE76677.1.
PIRiC64998.
RefSeqiNP_416772.1. NC_000913.3.

3D structure databases

ProteinModelPortaliQ47013.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261774. 14 interactions.
STRINGi511145.b2269.

Protein family/group databases

MEROPSiC79.001.

Proteomic databases

PaxDbiQ47013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75329; AAC75329; b2269.
BAE76677; BAE76677; BAE76677.
GeneIDi946742.
KEGGiecj:JW5840.
eco:b2269.
PATRICi32119905. VBIEscCol129921_2362.

Organism-specific databases

EchoBASEiEB4009.
EcoGeneiEG14261. elaD.

Phylogenomic databases

eggNOGiENOG4105Z6G. Bacteria.
ENOG410XTR6. LUCA.
HOGENOMiHOG000054843.
KOiK18015.
OMAiYRLSTPQ.

Enzyme and pathway databases

BioCyciEcoCyc:G7176-MONOMER.
ECOL316407:JW5840-MONOMER.
MetaCyc:G7176-MONOMER.

Miscellaneous databases

PROiQ47013.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELAD_ECOLI
AccessioniPrimary (citable) accession number: Q47013
Secondary accession number(s): P76480, Q2MAM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 4, 2007
Last modified: September 7, 2016
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.