Nitrogen assimilation regulatory protein nac - Escherichia coli (strain K12)

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Q47005 (NAC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nitrogen assimilation regulatory protein nac

Alternative name(s):
Nitrogen assimilation control protein

Gene names

Name:	nac
Ordered Locus Names:	b1988, JW1967

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression By similarity.
Sequence similarities	Contains 1 HTH lysR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Nitrate assimilation Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 11121068 Ref.1. Source: EcoCyc nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 11121068 Ref.1. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 11121068 Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 305

305

Nitrogen assimilation regulatory protein nac

PRO_0000105686

Regions

Domain

1 – 58

HTH lysR-type

DNA binding

18 – 37

H-T-H motif Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q47005 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B0B5DC408C6C6821
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FASTA

305

32,835

        10         20         30         40         50         60 
MNFRRLKYFV KIVDIGSLTQ AAEVLHIAQP ALSQQVATLE GELNQQLLIR TKRGVTPTDA 

        70         80         90        100        110        120 
GKILYTHARA ILRQCEQAQL AVHNVGQALS GQVSIGFAPG TAASSITMPL LQAVRAEFPE 

       130        140        150        160        170        180 
IVIYLHENSG AVLNEKLINH QLDMAVIYEH SPVAGVSSQA LLKEDLFLVG TQDCPGQSVD 

       190        200        210        220        230        240 
VNAIAQMNLF LPSDYSAIRL RVDEAFSLRR LTAKVIGEIE SIATLTAAIA SGMGVAVLPE 

       250        260        270        280        290        300 
SAARSLCGAV NGWMSRITTP SMSLSLSLNL PARANLSPQA QAVKELLMSV ISSPVMEKRQ 


WQLVS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nac (nitrogen assimilation control) gene from Escherichia coli." Muse W.B., Bender R.A. J. Bacteriol. 180:1166-1173(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A new gene, cbl, encoding a member of the LysR family of transcriptional regulators belongs to Escherichia coli cys regulon." Iwanicka-Nowicka R., Hryniewicz M.M. Gene 166:11-17(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 245-305. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U56736 Genomic DNA. Translation: AAD14869.1. U00096 Genomic DNA. Translation: AAC75050.1. AP009048 Genomic DNA. Translation: BAA15806.1. L31639 Genomic DNA. Translation: AAA99928.1.
PIR	D64963.
RefSeq	NP_416493.1. NC_000913.2. YP_490232.1. NC_007779.1.
3D structure databases
ProteinModelPortal	Q47005.
SMR	Q47005. Positions 1-241.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q47005. 1 interaction.
STRING	511145.b1988.
Protocols and materials databases
DNASU	946501.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75050; AAC75050; b1988. BAA15806; BAA15806; BAA15806.
GeneID	12931413. 946501.
KEGG	ecj:Y75_p1950. eco:b1988.
PATRIC	32119307. VBIEscCol129921_2064.
Organism-specific databases
EchoBASE	EB4013.
EcoGene	EG14265. nac.
Phylogenomic databases
eggNOG	COG0583.
HOGENOM	HOG000233514.
OMA	HENSGSV.
ProtClustDB	PRK11233.
Enzyme and pathway databases
BioCyc	EcoCyc:G7072-MONOMER. ECOL316407:JW1967-MONOMER.
Gene expression databases
Genevestigator	Q47005.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR005119. LysR_subst-bd. IPR000847. Tscrpt_reg_HTH_LysR. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00126. HTH_1. 1 hit. PF03466. LysR_substrate. 1 hit. [Graphical view]
PRINTS	PR00039. HTHLYSR.
PROSITE	PS50931. HTH_LYSR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAC_ECOLI

Accession

Primary (citable) accession number: Q47005
Secondary accession number(s): Q47082

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents