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Protein

Nitrogen assimilation regulatory protein nac

Gene

nac

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi18 – 3720H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Nitrate assimilation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7072-MONOMER.
ECOL316407:JW1967-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen assimilation regulatory protein nac
Alternative name(s):
Nitrogen assimilation control protein
Gene namesi
Name:nac
Ordered Locus Names:b1988, JW1967
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14265. nac.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Nitrogen assimilation regulatory protein nacPRO_0000105686Add
BLAST

Proteomic databases

PaxDbiQ47005.

Interactioni

Protein-protein interaction databases

BioGridi4260407. 8 interactions.
IntActiQ47005. 1 interaction.
STRINGi511145.b1988.

Structurei

3D structure databases

ProteinModelPortaliQ47005.
SMRiQ47005. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5858HTH lysR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4106P55. Bacteria.
ENOG410YGJ0. LUCA.
HOGENOMiHOG000233514.
InParanoidiQ47005.
KOiK19338.
OMAiYRHAQII.
OrthoDBiEOG686NGM.
PhylomeDBiQ47005.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q47005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFRRLKYFV KIVDIGSLTQ AAEVLHIAQP ALSQQVATLE GELNQQLLIR
60 70 80 90 100
TKRGVTPTDA GKILYTHARA ILRQCEQAQL AVHNVGQALS GQVSIGFAPG
110 120 130 140 150
TAASSITMPL LQAVRAEFPE IVIYLHENSG AVLNEKLINH QLDMAVIYEH
160 170 180 190 200
SPVAGVSSQA LLKEDLFLVG TQDCPGQSVD VNAIAQMNLF LPSDYSAIRL
210 220 230 240 250
RVDEAFSLRR LTAKVIGEIE SIATLTAAIA SGMGVAVLPE SAARSLCGAV
260 270 280 290 300
NGWMSRITTP SMSLSLSLNL PARANLSPQA QAVKELLMSV ISSPVMEKRQ

WQLVS
Length:305
Mass (Da):32,835
Last modified:November 1, 1996 - v1
Checksum:iB0B5DC408C6C6821
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56736 Genomic DNA. Translation: AAD14869.1.
U00096 Genomic DNA. Translation: AAC75050.1.
AP009048 Genomic DNA. Translation: BAA15806.1.
L31639 Genomic DNA. Translation: AAA99928.1.
PIRiD64963.
RefSeqiNP_416493.1. NC_000913.3.
WP_001011462.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75050; AAC75050; b1988.
BAA15806; BAA15806; BAA15806.
GeneIDi946501.
KEGGiecj:JW1967.
eco:b1988.
PATRICi32119307. VBIEscCol129921_2064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56736 Genomic DNA. Translation: AAD14869.1.
U00096 Genomic DNA. Translation: AAC75050.1.
AP009048 Genomic DNA. Translation: BAA15806.1.
L31639 Genomic DNA. Translation: AAA99928.1.
PIRiD64963.
RefSeqiNP_416493.1. NC_000913.3.
WP_001011462.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ47005.
SMRiQ47005. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260407. 8 interactions.
IntActiQ47005. 1 interaction.
STRINGi511145.b1988.

Proteomic databases

PaxDbiQ47005.

Protocols and materials databases

DNASUi946501.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75050; AAC75050; b1988.
BAA15806; BAA15806; BAA15806.
GeneIDi946501.
KEGGiecj:JW1967.
eco:b1988.
PATRICi32119307. VBIEscCol129921_2064.

Organism-specific databases

EchoBASEiEB4013.
EcoGeneiEG14265. nac.

Phylogenomic databases

eggNOGiENOG4106P55. Bacteria.
ENOG410YGJ0. LUCA.
HOGENOMiHOG000233514.
InParanoidiQ47005.
KOiK19338.
OMAiYRHAQII.
OrthoDBiEOG686NGM.
PhylomeDBiQ47005.

Enzyme and pathway databases

BioCyciEcoCyc:G7072-MONOMER.
ECOL316407:JW1967-MONOMER.

Miscellaneous databases

PROiQ47005.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nac (nitrogen assimilation control) gene from Escherichia coli."
    Muse W.B., Bender R.A.
    J. Bacteriol. 180:1166-1173(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A new gene, cbl, encoding a member of the LysR family of transcriptional regulators belongs to Escherichia coli cys regulon."
    Iwanicka-Nowicka R., Hryniewicz M.M.
    Gene 166:11-17(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 245-305.
    Strain: K12.

Entry informationi

Entry nameiNAC_ECOLI
AccessioniPrimary (citable) accession number: Q47005
Secondary accession number(s): Q47082
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: January 20, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.