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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

Reut_A1963

Organism
Cupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102SubstrateBy similarity1
Metal bindingi103Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandMetal-binding

Enzyme and pathway databases

BioCyciREUT264198:G12XR-1967-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:Reut_A1963
OrganismiCupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000002697 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000138681 – 165Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolaseAdd BLAST165

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi264198.Reut_A1963.

Structurei

3D structure databases

ProteinModelPortaliQ46ZV6.
SMRiQ46ZV6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 83Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiRSCDTQF.
OrthoDBiPOG091H03SM.

Family and domain databases

CDDicd16841. RraA_family. 1 hit.
Gene3Di3.50.30.40. 1 hit.
InterProiView protein in InterPro
IPR010203. RraA.
IPR005493. RraA/RraA-like.
IPR036704. RraA/RraA-like_sf.
PfamiView protein in Pfam
PF03737. RraA-like. 1 hit.
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46ZV6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHVTTDLCD ANEPRLADGT LRVMAPVFRA FGKQQAFAGP AATLKIFEDN
60 70 80 90 100
SLVREALESP GQGRVLVVDG GGSLRCALVG GNLGLLAEKN GWVGIIVNGC
110 120 130 140 150
VRDTGELDVC DIGIRALAAH PQKSQKRGAG ERDVAVQMPG AVVRPGNWIY
160
VDADGVLVSE EKLGA
Length:165
Mass (Da):17,319
Last modified:September 13, 2005 - v1
Checksum:iB221FBD451DCF8A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ61327.1.
RefSeqiWP_011298125.1. NC_007347.1.

Genome annotation databases

EnsemblBacteriaiAAZ61327; AAZ61327; Reut_A1963.
KEGGireu:Reut_A1963.

Similar proteinsi

Entry informationi

Entry nameiRRAAH_CUPNJ
AccessioniPrimary (citable) accession number: Q46ZV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: October 25, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families