Reviewed, UniProtKB/Swiss-Prot Q46ZJ3 (PURA1_RALEJ)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase 1
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase 1
    AdSS 1
    AMPSase 1

Gene names

Name:	purA1
Ordered Locus Names:	Reut_A2076

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Adenylosuccinate synthetase 1 HAMAP MF_00011		PRO_0000224312
Regions
	Nucleotide binding	20 – 26	7	GTP Potential
Sites
	Active site	148	1	By similarity
	Active site	155	1	By similarity
	Metal binding	21	1	Magnesium By similarity
	Metal binding	48	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46ZJ3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D7469D952A757324	FASTA	446	47,981
	10 20 30 40 50 60 MSASAVGQGR NVVVIGTQWG DEGKGKIVDW LTDHAKGVVR FQGGHNAGHT LIIGGKKTIL 70 80 90 100 110 120 RLIPSGIMRE GTVCYIGNGV VLSPEALFRE IEELETAGLE VQKRLRISEA ATLILPYHVA 130 140 150 160 170 180 IDKAREARRG AAKIGTTGRG IGPAYEDKVA RRALRVQDLF DPQQFAERLR ENLDFHNFML 190 200 210 220 230 240 TQYLGAEAVD YQQTLDDALA FAPRLAPMVA DVSAELYAVN AAGGNLMFEG AQGTLLDVDH 250 260 270 280 290 300 GTYPFVTSSN CVAGAAAAGA GVGPGRLSYI LGITKAYCTR VGAGPFPSEL YDNDNPARQD 310 320 330 340 350 360 QVGVRLANVG KEFGSVTGRP RRTGWLDAAA LKRSVQINGV SGLCLTKLDV LDGLESIKLC 370 380 390 400 410 420 VGYTLDGKTV DILPRGSDAV ARCEPVYEEF PGWNESTFGV KAWDALPEAA RVYLKRVEEV 430 440 VGIPIDMIST GPDRDETILL RHPYLA

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ61440.1.
RefSeq	YP_296284.1.
3D structure databases
SMR	Q46ZJ3. Positions 9-446.
ModBase	Search...
Genome annotation databases
GeneID	3611744.
GenomeReviews	Gene locus Reut_A2076 in contig CP000090_GR.
KEGG	reu:Reut_A2076.
NMPDR	fig|264198.3.peg.2762.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG658237.
OMA	KRGRLQQ.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_A2076-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA1_RALEJ

Accession

Primary (citable) accession number: Q46ZJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)