Q46Z82 (GLO2_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Reut_A2187

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000144792
Sites
	Metal binding	53	1	Zinc 1 By similarity
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	57	1	Zinc 2 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	118	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 1 By similarity
	Metal binding	140	1	Zinc 2 By similarity
	Metal binding	178	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46Z82 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 933FBA85F3632F25	FASTA	266	28,228
	10 20 30 40 50 60 MLKVEPIPAF QDNYIWAIHD GRNAAVVDPG EAAPVERFLK DKGLALGAIV ITHHHGDHQG 70 80 90 100 110 120 GVAELLSAYP TTHDGAPMPV IGPAGERIGG RTQAVREGDT VTLPAPALQF RVLDVPGHTA 130 140 150 160 170 180 GHVAYAGDLP GVGPVVFCGD TLFATGCGRL FEGTPAQMLA SLDKLASLPG ETRVYCAHEY 190 200 210 220 230 240 TRSNVRFARA VEPGNTALAD WEARVESLRE AGQPTVPSTI GLERQVNPFL RSREPTVREA 250 260 VKAQGGSVAG DAEAFGALRG WKDNFR

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ61551.1.
RefSeq	YP_296395.1. NC_007347.1.
3D structure databases
ProteinModelPortal	Q46Z82.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46Z82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3609614.
GenomeReviews	Gene locus Reut_A2187 in contig CP000090_GR.
KEGG	reu:Reut_A2187.
NMPDR	fig|264198.3.peg.2999.
PATRIC	20229977. VBIRalEut24049_2836.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	IGCGRVF.
ProtClustDB	CLSK896990.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_A2187-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_CUPPJ

Accession

Primary (citable) accession number: Q46Z82

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program