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Q46YU4

- MDH_CUPPJ

UniProt

Q46YU4 - MDH_CUPPJ

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Protein

Malate dehydrogenase

Gene

mdh

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931SubstrateUniRule annotation
Binding sitei99 – 991SubstrateUniRule annotation
Binding sitei106 – 1061NADUniRule annotation
Binding sitei113 – 1131NADUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Binding sitei163 – 1631SubstrateUniRule annotation
Active sitei188 – 1881Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187NADUniRule annotation
Nucleotide bindingi130 – 1323NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-2366-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Reut_A2327
OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002697: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 327326Malate dehydrogenasePRO_0000113390Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi264198.Reut_A2327.

Structurei

3D structure databases

ProteinModelPortaliQ46YU4.
SMRiQ46YU4. Positions 3-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiNCLIASK.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q46YU4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKAPMRVAV TGAAGQIGYS LLFRIANGDM LGKDQPVILQ LLDLPQAQAA
60 70 80 90 100
VKGVVMELED CAFPLLAGVV ITDDPKVAFK DADVALLVGA RPRSKGMERK
110 120 130 140 150
DLLEANAQIF TVQGKALDEV ASRNVKVLVV GNPANTNAYI AMKSAPSLPR
160 170 180 190 200
ENFTAMLRLD HNRALSQIAA KTGKPVASIE KMFVWGNHSP TMYADYRYAT
210 220 230 240 250
VDGKSVKDMI NDPVWNNDVF LPTVGKRGAA IIEARGLSSA ASAANAAIDH
260 270 280 290 300
VHDWVLGTNG KVVTMGIPSN GEYGIPADTM FGYPVTCANG KYEIVKGLEI
310 320
DAYSQEKINI TLNELEEEKA GVQHLLG
Length:327
Mass (Da):35,021
Last modified:September 13, 2005 - v1
Checksum:iDE9EC7B6386FC77C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ61689.1.
RefSeqiWP_011298478.1. NC_007347.1.
YP_296533.1. NC_007347.1.

Genome annotation databases

EnsemblBacteriaiAAZ61689; AAZ61689; Reut_A2327.
GeneIDi3610322.
KEGGireu:Reut_A2327.
PATRICi20230285. VBIRalEut24049_2983.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ61689.1 .
RefSeqi WP_011298478.1. NC_007347.1.
YP_296533.1. NC_007347.1.

3D structure databases

ProteinModelPortali Q46YU4.
SMRi Q46YU4. Positions 3-327.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264198.Reut_A2327.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ61689 ; AAZ61689 ; Reut_A2327 .
GeneIDi 3610322.
KEGGi reu:Reut_A2327.
PATRICi 20230285. VBIRalEut24049_2983.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000220953.
KOi K00024.
OMAi NCLIASK.
OrthoDBi EOG6PP9Q2.

Enzyme and pathway databases

BioCyci CPIN264198:GIW3-2366-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01517. Malate_dehydrog_2.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JMP134 / LMG 1197.

Entry informationi

Entry nameiMDH_CUPPJ
AccessioniPrimary (citable) accession number: Q46YU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3