Reviewed, UniProtKB/Swiss-Prot Q46YI9 (ILVD2_RALEJ)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 2
      Short name=DAD 2
    EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	Reut_A2432

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000225416
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	201	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q46YI9-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 6093841AD4B11C82	FASTA	619	66,305
	10 20 30 40 50 60 MPAYRSKTST AGRNMAGARS LWRATGMKDD DFNKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEAAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRDII ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPV IFVSGGPMEA GKTRLANPVT KAVEFKKLDL VDAMVIAADP 190 200 210 220 230 240 AYSDADVAEV ERSACPTCGS CSGMFTANSM NCLTEALGLS LPGNGTVVAT HADREQLFKR 250 260 270 280 290 300 AGSRIVELAR QYYEQEDARV LPRSVGFKAF ENAMTLDIAM GGSTNTILHL LAIAQEAGID 310 320 330 340 350 360 FSMNDIDRLS RVVPQLCKVA PNTNKYHIED VHRAGGIMAI LGELERAGKL HTDVPTVHAP 370 380 390 400 410 420 SLGDALNQWD IVRTKDEAVR HFYMAGPAGI PTQVAFSQNT RWPSLDLDRA EGCIRSYEHA 430 440 450 460 470 480 FSKEGGLAVL RGNIALDGCV VKTAGVDESI LVFEGSAHVT ESQDEAVENI LNDKVKAGDV 490 500 510 520 530 540 VIVRYEGPKG GPGMQEMLYP TSYIKSKGLG KACALLTDGR FSGGTSGLSI GHCSPEAAAG 550 560 570 580 590 600 GAIGLVRDGD KIRIDIPNRT IDVLVSDEEL ARRREEQNAK GWKPAKARPR KVSAALKAYA 610 KLVMSADKGA VRDLSLLDD

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000090 Genomic DNA. Translation: AAZ61794.1.
RefSeq	YP_296638.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3612300.
GenomeReviews	Gene locus Reut_A2432 in contig CP000090_GR.
KEGG	reu:Reut_A2432.
NMPDR	fig|264198.3.peg.3155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46YI9.
OMA	SRKVPCL.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_A2432-MON.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_RALEJ

Accession

Primary (citable) accession number: Q46YI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)