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Q46Y84 (TRMD_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:Reut_A2538
OrganismCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134)) [Complete proteome] [HAMAP]
Taxonomic identifier264198 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00605

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00605

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 260260tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00605
PRO_0000257453

Regions

Region137 – 1426S-adenosyl-L-methionine binding By similarity

Sites

Binding site1171S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46Y84 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 2666526C9DD7C78E

FASTA26029,051
        10         20         30         40         50         60 
MQFDVITLFP EMFRALTDWG ITSRAAKQQR YALRTWNPRD FTTDNYRTID DRPYGGGPGM 

        70         80         90        100        110        120 
VMLPRPLDDA IDAASAAQSQ AGVDTPHVVL MSPQGATLTH EKVMSLAQRP GLVLLCGRYE 

       130        140        150        160        170        180 
AIDQRLIDRR VHEEISLGDF VLSGGELPAM ALIDAVVRHL PGVLGDAQSA VQDSFVNGLL 

       190        200        210        220        230        240 
DCPHYTRPEE YEGVRVPEIL LGGHHAEIEK WRRQQALANT ARKRPDLIEA ARKQGLLTRA 

       250        260 
DEKFLSEWLV KEGREMSPAR 

« Hide

References

[1]"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000090 Genomic DNA. Translation: AAZ61899.1.
RefSeqYP_296743.1. NC_007347.1.

3D structure databases

ProteinModelPortalQ46Y84.
SMRQ46Y84. Positions 1-250.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264198.Reut_A2538.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ61899; AAZ61899; Reut_A2538.
GeneID3609310.
KEGGreu:Reut_A2538.
PATRIC20230736. VBIRalEut24049_3203.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0336.
HOGENOMHOG000016242.
KOK00554.
OMACGRFEGI.
OrthoDBEOG6J48RZ.
ProtClustDBPRK00026.

Enzyme and pathway databases

BioCycCPIN264198:GIW3-2582-MONOMER.

Family and domain databases

Gene3D1.10.1270.20. 1 hit.
HAMAPMF_00605. TrmD.
InterProIPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. trmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_CUPPJ
AccessionPrimary (citable) accession number: Q46Y84
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families