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Q46XM7

- SYI_CUPPJ

UniProt

Q46XM7 - SYI_CUPPJ

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei596 – 5961Aminoacyl-adenylateUniRule annotation
    Binding sitei640 – 6401ATPUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation
    Metal bindingi946 – 9461ZincUniRule annotation
    Metal bindingi949 – 9491ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCPIN264198:GIW3-2792-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Reut_A2745
    OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
    Taxonomic identifieri264198 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000002697: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 963963Isoleucine--tRNA ligasePRO_0000098452Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi264198.Reut_A2745.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46XM7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi66 – 7611"HIGH" regionAdd
    BLAST
    Motifi637 – 6415"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46XM7-1 [UniParc]FASTAAdd to Basket

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    MSDDKRAKPE KSKYPVNLLD TTFPMRGDLP KREPQWVKQW QDKQLYKKIR    50
    AARKGAKKFV LHDGPPYANG DIHIGHAVNK VLKDMIVKAR GLSGLDAVYV 100
    PGWDCHGMPI EIQIEKQFGK GLPVQEVQAK ARAYATEQIK RQMVDFERLG 150
    VLGDWGHPYL TMNYSNEADE LRALGKIMEK GYVFRGLKPV NWCFDCGSAL 200
    AEAEVEYKDK VDLSIDVGFP FAETDKLAHA FKLSIEQLNA KPGWIVIWTT 250
    TPWTIPSNQA LNVHPEVEYA LVDTPRGYLI LATERVEEQL KIYELEGKVV 300
    ATTTGAALSE IRFHHPLAKM DTGYDRLSPI YLGDYVTTDT GSGIVHSAPA 350
    YGVEDFQSCK AHGMSDHDII SPVMGNGVYA GTLPLFGGLS IWDANPKIVE 400
    VLKASGNLFN SHKYTHSYMH CWRHKTPIIY RATSQWFAGM DVDPVEQDGK 450
    AVPTLRETAL AGIEATEFYP SWGKQRLHNM IANRPDWTLS RQRQWGVPMA 500
    FFVHKETGAL HPRTAELLEE VARRVEQHGI EAWQTLDPKD LLGDEADQYE 550
    KNRDTLDVWF DSGTTHWTVI RGSHRDDLYD PSADEADGRL ADLYLEGSDQ 600
    HRGWFHSSLL TASMLYGKPP YKALLTHGFT VDGEGRKMSK SVGNTVSPQD 650
    IANKMGAEII RLWVASTDYS GELSISDEIL KRVVESYRRI RNTLRFLLSN 700
    LSDYDHSKHA LPASEWLEID RYAVALTERL QKEVLSHYDS YEFHPVVAKL 750
    QTFCSEDLGG FYLDVLKDRL YTTAADSKAR RAAQNALYHI TQAMLHWMAP 800
    FLSFTAEEAW QVFAHGTGHT DTIFTSTYYT LPEVDQADDL LQKWHSLREV 850
    RAEVTKQLEA VRVEGAIGSS LQAEVNIQAG GPVLAALQSL EDDLRFVLLT 900
    SAATVTPAPE AGDLLVTVTA STHAKCERCW HYRADVGQNP DHPTLCGRCD 950
    SNLFGAGEHR SHA 963
    Length:963
    Mass (Da):108,201
    Last modified:December 20, 2005 - v2
    Checksum:iE3CFF0F4E3D78986
    GO

    Sequence cautioni

    The sequence AAZ62106.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000090 Genomic DNA. Translation: AAZ62106.1. Different initiation.
    RefSeqiYP_296950.1. NC_007347.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ62106; AAZ62106; Reut_A2745.
    GeneIDi3612451.
    KEGGireu:Reut_A2745.
    PATRICi20231166. VBIRalEut24049_3416.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000090 Genomic DNA. Translation: AAZ62106.1 . Different initiation.
    RefSeqi YP_296950.1. NC_007347.1.

    3D structure databases

    ProteinModelPortali Q46XM7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264198.Reut_A2745.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ62106 ; AAZ62106 ; Reut_A2745 .
    GeneIDi 3612451.
    KEGGi reu:Reut_A2745.
    PATRICi 20231166. VBIRalEut24049_3416.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CPIN264198:GIW3-2792-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JMP134 / LMG 1197.

    Entry informationi

    Entry nameiSYI_CUPPJ
    AccessioniPrimary (citable) accession number: Q46XM7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3