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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961Aminoacyl-adenylateUniRule annotation
Binding sitei640 – 6401ATPUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation
Metal bindingi946 – 9461ZincUniRule annotation
Metal bindingi949 – 9491ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-2792-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Reut_A2745
OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002697 Componenti: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 963963Isoleucine--tRNA ligasePRO_0000098452Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264198.Reut_A2745.

Structurei

3D structure databases

ProteinModelPortaliQ46XM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionAdd
BLAST
Motifi637 – 6415"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46XM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDKRAKPE KSKYPVNLLD TTFPMRGDLP KREPQWVKQW QDKQLYKKIR
60 70 80 90 100
AARKGAKKFV LHDGPPYANG DIHIGHAVNK VLKDMIVKAR GLSGLDAVYV
110 120 130 140 150
PGWDCHGMPI EIQIEKQFGK GLPVQEVQAK ARAYATEQIK RQMVDFERLG
160 170 180 190 200
VLGDWGHPYL TMNYSNEADE LRALGKIMEK GYVFRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDK VDLSIDVGFP FAETDKLAHA FKLSIEQLNA KPGWIVIWTT
260 270 280 290 300
TPWTIPSNQA LNVHPEVEYA LVDTPRGYLI LATERVEEQL KIYELEGKVV
310 320 330 340 350
ATTTGAALSE IRFHHPLAKM DTGYDRLSPI YLGDYVTTDT GSGIVHSAPA
360 370 380 390 400
YGVEDFQSCK AHGMSDHDII SPVMGNGVYA GTLPLFGGLS IWDANPKIVE
410 420 430 440 450
VLKASGNLFN SHKYTHSYMH CWRHKTPIIY RATSQWFAGM DVDPVEQDGK
460 470 480 490 500
AVPTLRETAL AGIEATEFYP SWGKQRLHNM IANRPDWTLS RQRQWGVPMA
510 520 530 540 550
FFVHKETGAL HPRTAELLEE VARRVEQHGI EAWQTLDPKD LLGDEADQYE
560 570 580 590 600
KNRDTLDVWF DSGTTHWTVI RGSHRDDLYD PSADEADGRL ADLYLEGSDQ
610 620 630 640 650
HRGWFHSSLL TASMLYGKPP YKALLTHGFT VDGEGRKMSK SVGNTVSPQD
660 670 680 690 700
IANKMGAEII RLWVASTDYS GELSISDEIL KRVVESYRRI RNTLRFLLSN
710 720 730 740 750
LSDYDHSKHA LPASEWLEID RYAVALTERL QKEVLSHYDS YEFHPVVAKL
760 770 780 790 800
QTFCSEDLGG FYLDVLKDRL YTTAADSKAR RAAQNALYHI TQAMLHWMAP
810 820 830 840 850
FLSFTAEEAW QVFAHGTGHT DTIFTSTYYT LPEVDQADDL LQKWHSLREV
860 870 880 890 900
RAEVTKQLEA VRVEGAIGSS LQAEVNIQAG GPVLAALQSL EDDLRFVLLT
910 920 930 940 950
SAATVTPAPE AGDLLVTVTA STHAKCERCW HYRADVGQNP DHPTLCGRCD
960
SNLFGAGEHR SHA
Length:963
Mass (Da):108,201
Last modified:December 20, 2005 - v2
Checksum:iE3CFF0F4E3D78986
GO

Sequence cautioni

The sequence AAZ62106.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ62106.1. Different initiation.
RefSeqiYP_296950.1. NC_007347.1.

Genome annotation databases

EnsemblBacteriaiAAZ62106; AAZ62106; Reut_A2745.
KEGGireu:Reut_A2745.
PATRICi20231166. VBIRalEut24049_3416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ62106.1. Different initiation.
RefSeqiYP_296950.1. NC_007347.1.

3D structure databases

ProteinModelPortaliQ46XM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264198.Reut_A2745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ62106; AAZ62106; Reut_A2745.
KEGGireu:Reut_A2745.
PATRICi20231166. VBIRalEut24049_3416.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-2792-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JMP134 / LMG 1197.

Entry informationi

Entry nameiSYI_CUPPJ
AccessioniPrimary (citable) accession number: Q46XM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 1, 2015
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.