Q46XE1 (PROA_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Reut_A2831

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus pinatubonensis

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP-Rule MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP-Rule MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP-Rule MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Gamma-glutamyl phosphate reductase HAMAP-Rule MF_00412		PRO_0000230019

Sequences

	Sequence		Length	Mass (Da)
	Q46XE1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: F7EF7346E22C43C2	FASTA	426	45,898
	10 20 30 40 50 60 MTELDLNQYM DRVGRQARAA SRAMARASTA DKNRALLTIA AAIRRDADKL KAVNARDVER 70 80 90 100 110 120 ARANGQDAAF VDRLTLSDKA IKTMAEGLEQ IAALADPIGE ISNMKFRPTG IQVGQMRVPL 130 140 150 160 170 180 GVIGIIYESR PNVTIDAAAL CLKSGNATIL RGGSEAIESN TALAALVAEG LASAGLPPEA 190 200 210 220 230 240 VQVVETTDRA AVGRLITMTE YVDVIVPRGG KSLIARLMEE ARVPMIKHLD GICHVFIDAD 250 260 270 280 290 300 ADLDKAVRVC DNAKTQRYAP CNTMETLLVS QDIAARALPP LCRIYQEKGV ELRVCPATRA 310 320 330 340 350 360 MLEAAGFSGL VDAHEEDWRL EYLAPILAIK TVAGLDEAIA HINEYGSHHT DSIITENYST 370 380 390 400 410 420 GMRFIREVDS ASVMINASTR FADGFEYGLG AEIGISNDKL HARGPVGLEG LTSLKYVVFG HGEIRT

References

[1]

"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000090 Genomic DNA. Translation: AAZ62192.1.
RefSeq	YP_297036.1. NC_007347.1.
3D structure databases
ProteinModelPortal	Q46XE1.
ModBase	Search...
Protein-protein interaction databases
STRING	264198.Reut_A2831.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ62192; AAZ62192; Reut_A2831.
GeneID	3612191.
KEGG	reu:Reut_A2831.
PATRIC	20231348. VBIRalEut24049_3507.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HOG000246356.
KO	K00147.
OMA	EVGISTQ.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	CPIN264198:GIW3-2878-MONOMER.
UniPathway	UPA00098; UER00360.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 2 hits.
HAMAP	MF_00412. ProA.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
PANTHER	PTHR11063:SF1. PTHR11063:SF1. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CUPPJ

Accession

Primary (citable) accession number: Q46XE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program