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Protein

Gamma-glutamyl phosphate reductase

Gene

proA

Organism
Cupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.UniRule annotation

Catalytic activityi

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00098; UER00360

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl phosphate reductaseUniRule annotation (EC:1.2.1.41UniRule annotation)
Short name:
GPRUniRule annotation
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenaseUniRule annotation
Glutamyl-gamma-semialdehyde dehydrogenaseUniRule annotation
Short name:
GSA dehydrogenaseUniRule annotation
Gene namesi
Name:proAUniRule annotation
Ordered Locus Names:Reut_A2831
OrganismiCupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000002697 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002300191 – 426Gamma-glutamyl phosphate reductaseAdd BLAST426

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_A2831

Structurei

3D structure databases

ProteinModelPortaliQ46XE1
SMRiQ46XE1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gamma-glutamyl phosphate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C2S Bacteria
COG0014 LUCA
HOGENOMiHOG000246356
KOiK00147
OMAiHGSHHTD
OrthoDBiPOG091H00OT

Family and domain databases

CDDicd07079 ALDH_F18-19_ProA-GPR, 1 hit
Gene3Di3.40.309.10, 1 hit
3.40.605.10, 2 hits
HAMAPiMF_00412 ProA, 1 hit
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
IPR020593 G-glutamylP_reductase_CS
IPR012134 Glu-5-SA_DH
IPR000965 GPR_dom
PfamiView protein in Pfam
PF00171 Aldedh, 2 hits
PIRSFiPIRSF000151 GPR, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR00407 proA, 1 hit
PROSITEiView protein in PROSITE
PS01223 PROA, 1 hit

Sequencei

Sequence statusi: Complete.

Q46XE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELDLNQYM DRVGRQARAA SRAMARASTA DKNRALLTIA AAIRRDADKL
60 70 80 90 100
KAVNARDVER ARANGQDAAF VDRLTLSDKA IKTMAEGLEQ IAALADPIGE
110 120 130 140 150
ISNMKFRPTG IQVGQMRVPL GVIGIIYESR PNVTIDAAAL CLKSGNATIL
160 170 180 190 200
RGGSEAIESN TALAALVAEG LASAGLPPEA VQVVETTDRA AVGRLITMTE
210 220 230 240 250
YVDVIVPRGG KSLIARLMEE ARVPMIKHLD GICHVFIDAD ADLDKAVRVC
260 270 280 290 300
DNAKTQRYAP CNTMETLLVS QDIAARALPP LCRIYQEKGV ELRVCPATRA
310 320 330 340 350
MLEAAGFSGL VDAHEEDWRL EYLAPILAIK TVAGLDEAIA HINEYGSHHT
360 370 380 390 400
DSIITENYST GMRFIREVDS ASVMINASTR FADGFEYGLG AEIGISNDKL
410 420
HARGPVGLEG LTSLKYVVFG HGEIRT
Length:426
Mass (Da):45,898
Last modified:September 13, 2005 - v1
Checksum:iF7EF7346E22C43C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA Translation: AAZ62192.1
RefSeqiWP_011298977.1, NC_007347.1

Genome annotation databases

EnsemblBacteriaiAAZ62192; AAZ62192; Reut_A2831
KEGGireu:Reut_A2831

Similar proteinsi

Entry informationi

Entry nameiPROA_CUPNJ
AccessioniPrimary (citable) accession number: Q46XE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health