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Q46XD8 (SYL_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Reut_A2834
OrganismCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134)) [Complete proteome] [HAMAP]
Taxonomic identifier264198 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length873 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAZ62195.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 873873Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334800

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_00049
Motif636 – 6405"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6391ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46XD8 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 64987B16A46F6342

FASTA87397,066
        10         20         30         40         50         60 
MQDKYLPSAV EQAAQQHWQA IDAYRVPEHA TGPDGKEKPK FYACSMLPYP SGKLHMGHVR 

        70         80         90        100        110        120 
NYTINDVMAR YLRMNGNNVL MPMGWDAFGM PAENAALNNG VAPAAWTYDN IAYMKKQMQS 

       130        140        150        160        170        180 
MGLAIDWSRE VATCSPDYYR WNQWLFLKML EKGIAYRKTG TVNWDPVDQT VLANEQVIDG 

       190        200        210        220        230        240 
RGWRSGAIVE KREIPMYYLR ITDYAEELLG DLDGLGWPER VKIMQQNWIG KSVGVRFAFK 

       250        260        270        280        290        300 
HDIEGDDGQL INDGKLYVFT TRADTIMGVT FCAVAAEHPL ATHAAANNPE LAAFIDECKH 

       310        320        330        340        350        360 
GSVMEADMAT MEKKGMPTGL QVTHPLTGEQ VDVWVGNYVL MSYGDGAVMG VPAHDERDFA 

       370        380        390        400        410        420 
FANKYKLPIR QVIDVKGQPY STEAWQEWYG DKENGVCIES GKYNGLGYLA AVEAIAADLG 

       430        440        450        460        470        480 
AMGLGEKKIT WRLRDWGISR QRYWGTPIPL IHCDSCGVVP VPEQDLPVVL PEDLVPDGTG 

       490        500        510        520        530        540 
NPLNKDPRFL QCSCPSCGKP ARRETDTMDT FIDSCWYYMR YTCPDAATMV DARNDYWMPM 

       550        560        570        580        590        600 
DQYIGGIEHA ILHLLYARFW TKVMRDLGLV KFDEPFTNLL TQGMVLNETF YREDAAGKKT 

       610        620        630        640        650        660 
WYNPADVDVQ TDDRGRPVGA TLKADGQPVV IGGVEKMSKS KNNGIDPQAL IDQHGADTAR 

       670        680        690        700        710        720 
LFVMFAAPPE QQLEWSGSGV EGASRFLRRV WNYGFANAAA VRDGAGAAPT ADDADLRREI 

       730        740        750        760        770        780 
HGVLKQANYD YQRIQYNTVV SATMKMLNAL EDAKNASPVA RRECFGILLR VLYPVVPHIT 

       790        800        810        820        830        840 
HGLWDALGYA TQYGDLLDAP WPQVDEGALV RTEIEMVLQI NGKVRGSVTV PADADRAAIE 

       850        860        870 
TAAAASETVA KFAEGKAPKK IVVVPGRLVN VVL 

« Hide

References

[1]"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000090 Genomic DNA. Translation: AAZ62195.1. Different initiation.
RefSeqYP_297039.2. NC_007347.1.

3D structure databases

ProteinModelPortalQ46XD8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264198.Reut_A2834.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ62195; AAZ62195; Reut_A2834.
GeneID3612136.
KEGGreu:Reut_A2834.
PATRIC20231354. VBIRalEut24049_3510.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCPIN264198:GIW3-2881-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CUPPJ
AccessionPrimary (citable) accession number: Q46XD8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries