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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei639 – 6391ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-2881-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Reut_A2834
OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002697: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Leucine--tRNA ligasePRO_0000334800Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_A2834.

Structurei

3D structure databases

ProteinModelPortaliQ46XD8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi636 – 6405"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46XD8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQDKYLPSAV EQAAQQHWQA IDAYRVPEHA TGPDGKEKPK FYACSMLPYP
60 70 80 90 100
SGKLHMGHVR NYTINDVMAR YLRMNGNNVL MPMGWDAFGM PAENAALNNG
110 120 130 140 150
VAPAAWTYDN IAYMKKQMQS MGLAIDWSRE VATCSPDYYR WNQWLFLKML
160 170 180 190 200
EKGIAYRKTG TVNWDPVDQT VLANEQVIDG RGWRSGAIVE KREIPMYYLR
210 220 230 240 250
ITDYAEELLG DLDGLGWPER VKIMQQNWIG KSVGVRFAFK HDIEGDDGQL
260 270 280 290 300
INDGKLYVFT TRADTIMGVT FCAVAAEHPL ATHAAANNPE LAAFIDECKH
310 320 330 340 350
GSVMEADMAT MEKKGMPTGL QVTHPLTGEQ VDVWVGNYVL MSYGDGAVMG
360 370 380 390 400
VPAHDERDFA FANKYKLPIR QVIDVKGQPY STEAWQEWYG DKENGVCIES
410 420 430 440 450
GKYNGLGYLA AVEAIAADLG AMGLGEKKIT WRLRDWGISR QRYWGTPIPL
460 470 480 490 500
IHCDSCGVVP VPEQDLPVVL PEDLVPDGTG NPLNKDPRFL QCSCPSCGKP
510 520 530 540 550
ARRETDTMDT FIDSCWYYMR YTCPDAATMV DARNDYWMPM DQYIGGIEHA
560 570 580 590 600
ILHLLYARFW TKVMRDLGLV KFDEPFTNLL TQGMVLNETF YREDAAGKKT
610 620 630 640 650
WYNPADVDVQ TDDRGRPVGA TLKADGQPVV IGGVEKMSKS KNNGIDPQAL
660 670 680 690 700
IDQHGADTAR LFVMFAAPPE QQLEWSGSGV EGASRFLRRV WNYGFANAAA
710 720 730 740 750
VRDGAGAAPT ADDADLRREI HGVLKQANYD YQRIQYNTVV SATMKMLNAL
760 770 780 790 800
EDAKNASPVA RRECFGILLR VLYPVVPHIT HGLWDALGYA TQYGDLLDAP
810 820 830 840 850
WPQVDEGALV RTEIEMVLQI NGKVRGSVTV PADADRAAIE TAAAASETVA
860 870
KFAEGKAPKK IVVVPGRLVN VVL
Length:873
Mass (Da):97,066
Last modified:May 20, 2008 - v2
Checksum:i64987B16A46F6342
GO

Sequence cautioni

The sequence AAZ62195.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ62195.1. Different initiation.
RefSeqiYP_297039.2. NC_007347.1.

Genome annotation databases

EnsemblBacteriaiAAZ62195; AAZ62195; Reut_A2834.
GeneIDi3612136.
KEGGireu:Reut_A2834.
PATRICi20231354. VBIRalEut24049_3510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ62195.1. Different initiation.
RefSeqiYP_297039.2. NC_007347.1.

3D structure databases

ProteinModelPortaliQ46XD8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264198.Reut_A2834.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ62195; AAZ62195; Reut_A2834.
GeneIDi3612136.
KEGGireu:Reut_A2834.
PATRICi20231354. VBIRalEut24049_3510.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-2881-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JMP134 / LMG 1197.

Entry informationi

Entry nameiSYL_CUPPJ
AccessioniPrimary (citable) accession number: Q46XD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.