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Protein

Glutamate 5-kinase

Gene

proB

Organism
Cupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14ATPUniRule annotation1
Binding sitei54SubstrateUniRule annotation1
Binding sitei141SubstrateUniRule annotation1
Binding sitei153Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi173 – 174ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Reut_A2955
OrganismiCupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000002697 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002300621 – 372Glutamate 5-kinaseAdd BLAST372

Proteomic databases

PRIDEiQ46X18

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_A2955

Structurei

3D structure databases

ProteinModelPortaliQ46X18
SMRiQ46X18
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 358PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q46X18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSVIAQAKR IVVKVGSSLV TNDGKGLDYD AIARWAAQIA KLRSIGKEVV
60 70 80 90 100
LVSSGAIAEG MQRLGWVRRP REIHELQAAA AVGQMGLAQV YESQFGRYGI
110 120 130 140 150
RTAQVLLTHA DLADRERYLN ARSTLLTLLS LGVVPIINEN DTVVTDEIKF
160 170 180 190 200
GDNDTLGALV TNLIEGDALV ILTDQRGLYT ADPRKDPNAE FVDEALAGTP
210 220 230 240 250
ELEQMAGGAG TSIGRGGMLT KILAAKRAAK SGAHTTIASG REANVLERLA
260 270 280 290 300
AGEAIGTQLL APTGRLTARK QWMADHLQLR GRVVIDAGAV EKLTSGGKSL
310 320 330 340 350
LPIGVVEVQG EFARGEVIAC VGADGREVAR GITNYSSAEA RLIARKPSSE
360 370
IESVLGHLNE PELIHRDNLV LV
Length:372
Mass (Da):39,560
Last modified:September 13, 2005 - v1
Checksum:iB5D5DA9B384B6D6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA Translation: AAZ62315.1
RefSeqiWP_011299100.1, NC_007347.1

Genome annotation databases

EnsemblBacteriaiAAZ62315; AAZ62315; Reut_A2955
KEGGireu:Reut_A2955

Similar proteinsi

Entry informationi

Entry nameiPROB_CUPNJ
AccessioniPrimary (citable) accession number: Q46X18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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