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Q46WZ6 (DDL_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Reut_A2977
OrganismCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134)) [Complete proteome] [HAMAP]
Taxonomic identifier264198 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341159

Regions

Domain113 – 312200ATP-grasp
Nucleotide binding139 – 19456ATP By similarity

Sites

Metal binding2661Magnesium or manganese 1 By similarity
Metal binding2791Magnesium or manganese 1 By similarity
Metal binding2791Magnesium or manganese 2 By similarity
Metal binding2811Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46WZ6 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: A05938A5B9B332BE

FASTA32734,991
        10         20         30         40         50         60 
MSFVANPKID PKSLGKVGVL YGGRSAEREI SLMSGSGVLA ALQSRGVDAH GFDPATQSVA 

        70         80         90        100        110        120 
ELAAAGFDRV FIALHGRYGE DGTMQGLLEQ LGVPYTGSGV LASALAMDKQ ATKRLWMTHD 

       130        140        150        160        170        180 
LSTPRFAMLY ADTDFDAVVA DLGLPLIVKP AREGSSIGLS KVTDASQMRE AFEKAAALDN 

       190        200        210        220        230        240 
DVIAETFIDG AELTCPLVGE GATAEALPVI RIVAPDSNYD YQNKYFTDDT QYLCPSGLSA 

       250        260        270        280        290        300 
EVEREVQQLA VQAYRVLGCR GWARADVMLR ADGKPFLLEM NTSPGMTGHS LVPMAARAAG 

       310        320 
ISYEDFVMQV VAAATLDLHP NEHWKPE 

« Hide

References

[1]"Complete sequence of chromosome 1 of Ralstonia eutropha JMP134."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000090 Genomic DNA. Translation: AAZ62337.1.
RefSeqYP_297181.1. NC_007347.1.

3D structure databases

ProteinModelPortalQ46WZ6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264198.Reut_A2977.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ62337; AAZ62337; Reut_A2977.
GeneID3612374.
KEGGreu:Reut_A2977.
PATRIC20231638. VBIRalEut24049_3652.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycCPIN264198:GIW3-3025-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CUPPJ
AccessionPrimary (citable) accession number: Q46WZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways