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Protein

Shikimate kinase

Gene

aroK

Organism
Cupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.UniRule annotation

Catalytic activityi

ATP + shikimate = ADP + shikimate 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (Reut_A1026), Phospho-2-dehydro-3-deoxyheptonate aldolase (Reut_B4281)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi19MagnesiumUniRule annotation1
Binding sitei37SubstrateUniRule annotation1
Binding sitei61SubstrateUniRule annotation1
Binding sitei83Substrate; via amide nitrogenUniRule annotation1
Binding sitei121ATPUniRule annotation1
Binding sitei140SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 20ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate kinaseUniRule annotation (EC:2.7.1.71UniRule annotation)
Short name:
SKUniRule annotation
Gene namesi
Name:aroKUniRule annotation
Ordered Locus Names:Reut_A3130
OrganismiCupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000002697 Componenti: Chromosome 1

Subcellular locationi

Q46WJ3:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002379171 – 192Shikimate kinaseAdd BLAST192

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264198.Reut_A3130.

Structurei

3D structure databases

ProteinModelPortaliQ46WJ3.
SMRiQ46WJ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the shikimate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
HOGENOMiHOG000032568.
KOiK00891.
OMAiHGEPHFR.
OrthoDBiPOG091H02J5.

Family and domain databases

CDDicd00464. SK. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
PfamiView protein in Pfam
PF01202. SKI. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS01128. SHIKIMATE_KINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46WJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METGRPNLFF VGLMGAGKTT VGRTVARRLH YPFFDSDHEL EAHCGVRIPV
60 70 80 90 100
IFEHEGEEGF RDREAAMIRE LTGRSGIVLA TGGGAVLRPE NRELLKSRGT
110 120 130 140 150
VVYLRASPHD LWLRTRHDRN RPLLQTEDPK GKLEALYGAR DPLYREVADF
160 170 180 190
IIETGKPSVA QLANMVLMQL EMAGFRIDTA AASDQPANDT HS
Length:192
Mass (Da):21,409
Last modified:September 13, 2005 - v1
Checksum:iD6D4180EABFDA7F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000090 Genomic DNA. Translation: AAZ62490.1.
RefSeqiWP_011299273.1. NC_007347.1.

Genome annotation databases

EnsemblBacteriaiAAZ62490; AAZ62490; Reut_A3130.
KEGGireu:Reut_A3130.

Similar proteinsi

Entry informationi

Entry nameiAROK_CUPNJ
AccessioniPrimary (citable) accession number: Q46WJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: September 13, 2005
Last modified: October 25, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families