Reviewed, UniProtKB/Swiss-Prot Q46V28 (CHEB1_RALEJ)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Reut_B3648

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225479
Regions
	Domain	2 – 117	116	Response regulatory
	Domain	146 – 336	191	CheB-type methylesterase
Sites
	Active site	158	1	By similarity
	Active site	185	1	By similarity
	Active site	278	1	By similarity
Amino acid modifications
	Modified residue	53	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46V28-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 1A1C222071EFA7B4	FASTA	341	35,278
	10 20 30 40 50 60 MKVGIVNDSA LAVAALRRAI ALDPAFEIVW VAGDGEQAVQ LAASHTPDVI LMDLLMPVMD 70 80 90 100 110 120 GVEATRRIMA ATPCPIVVVT TDLGRNATQV FDAMGHGAID AVDTPTLTES DAKLTAGPLL 130 140 150 160 170 180 RKMRNIGRLV AGRAAPRPAI TEMPGTLAAS RLVAIGASAG GPAALAKLLG ALPADFCAAV 190 200 210 220 230 240 VAVQHVDEAF APGMADWLDS QCALPVRVAA PGETPQAGTV LIAGTNNHLR LLGSNRMAYT 250 260 270 280 290 300 EQPSEYLYRP SIDVFFESVV EYWRGQAIGV LLTGMGRDGA SGLKAMRDHG CLTIAQDQAT 310 320 330 340 SAVYGMPKAA AAMGAASEIL PLTAIAPRLV QACCSVRPPP G

References

[1]

"Complete sequence of chromosome 2 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000091 Genomic DNA. Translation: AAZ63006.1.
RefSeq	YP_297850.1.
3D structure databases
SMR	Q46V28. Positions 1-337.
ModBase	Search...
Genome annotation databases
GeneID	3613877.
GenomeReviews	Gene locus Reut_B3648 in contig CP000091_GR.
KEGG	reu:Reut_B3648.
NMPDR	fig|264198.3.peg.4546.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG705324.
OMA	FFESVVE.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_B3648-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_RALEJ

Accession

Primary (citable) accession number: Q46V28

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)