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Protein

Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase

Gene

phnN

Organism
Cupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP).By similarity

Catalytic activityi

ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate.
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase (phnN), Ribose 1,5-bisphosphate phosphokinase PhnN (phnN)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Multifunctional enzyme, Transferase
Biological processAlkylphosphonate uptake
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00087; UER00175

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase
Including the following 2 domains:
Ribose 1,5-bisphosphate phosphokinase PhnN (EC:2.7.4.23)
Alternative name(s):
Ribose 1,5-bisphosphokinase
Putative thymidine phosphorylase (EC:2.4.2.4)
Alternative name(s):
TdRPase
Gene namesi
Name:phnN
Ordered Locus Names:Reut_B3746
OrganismiCupriavidus necator (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000002697 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003147101 – 602Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylaseAdd BLAST602

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_B3746

Structurei

3D structure databases

ProteinModelPortaliQ46UT0
SMRiQ46UT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 187Ribose 1,5-bisphosphokinaseAdd BLAST187
Regioni188 – 602ThymidinephosphorylaseAdd BLAST415

Sequence similaritiesi

In the N-terminal section; belongs to the ribose 1,5-bisphosphokinase family.Curated
In the C-terminal section; belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CMW Bacteria
COG0213 LUCA
HOGENOMiHOG000252767
KOiK00758
OMAiVHSIGGV
OrthoDBiPOG091H02BC

Family and domain databases

Gene3Di3.40.1030.102 hits
3.90.1170.301 hit
HAMAPiMF_00836 PhnN, 1 hit
MF_00703 Thymid_phosp_2, 1 hit
InterProiView protein in InterPro
IPR000312 Glycosyl_Trfase_fam3
IPR036320 Glycosyl_Trfase_fam3_N_dom)sf
IPR035902 Nuc_phospho_transferase
IPR027417 P-loop_NTPase
IPR012699 PhnN
IPR036566 PYNP-like_C_sf
IPR013102 PYNP_C
IPR017872 Pyrmidine_PPase_CS
IPR028579 Thym_Pase_Put
IPR000053 Thymidine/pyrmidine_PPase
PANTHERiPTHR10515 PTHR10515, 1 hit
PfamiView protein in Pfam
PF00591 Glycos_transf_3, 1 hit
PF07831 PYNP_C, 1 hit
SMARTiView protein in SMART
SM00941 PYNP_C, 1 hit
SUPFAMiSSF47648 SSF47648, 1 hit
SSF52418 SSF52418, 1 hit
SSF52540 SSF52540, 1 hit
SSF54680 SSF54680, 1 hit
TIGRFAMsiTIGR02322 phosphon_PhnN, 1 hit
PROSITEiView protein in PROSITE
PS00647 THYMID_PHOSPHORYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q46UT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKESGTFFLV VGPSGAGKDS LIDGARATLG NDEYVFARRI ITRPSGSPGE
60 70 80 90 100
DHESVAEAEF AERERNGEFL VTWHAHGLRY GLPQWLVGLL ETGKHVVANG
110 120 130 140 150
SRGVIAMLAR QLPRFVVVNV TAPQDVLAQR IAARGRESGD DVMRRVARQA
160 170 180 190 200
PPMPDGVHCV TVTNDSTLDL GVARFTDALR NGANAGSVPQ PASRRHLAAK
210 220 230 240 250
LDGQPLDEGA YEAILRDAIA GRYTAQELTA FLTAATRSLD DREVVALARA
260 270 280 290 300
RTRFTARIEW DEPIVVDKHS MGGIPGSRIT LIVVPIVAAY GLAMPKTSSR
310 320 330 340 350
AITSAAGTAD AMETVARVDL THDDVRRCVA QARACIAWNG RLNHSVIDDV
360 370 380 390 400
MNAITRPLGL DSRRWAVASI LSKKATAGAT HVIVDIPYGP QTKLSARADA
410 420 430 440 450
DALAGLFEEV GKGLGLHVRA LVTDGSRPIG RGVGPALEVR DVRQVLENHP
460 470 480 490 500
DAPMDLREKA LRFAGEIIAF DPRVASAAQG MRIATALLDE GSARAAFDRI
510 520 530 540 550
VATQGIRPDP VAPGAHTHVI VAPAEGRVAA INGWQISGIA RAAGAPRSAG
560 570 580 590 600
AGIDLLCTIG ERVAAGEPLY RIHAESAADL ATAVAMTGPA GEASTAVRVD

PD
Length:602
Mass (Da):63,580
Last modified:January 15, 2008 - v2
Checksum:iD622AFD8F873E7CA
GO

Sequence cautioni

The sequence AAZ63104 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000091 Genomic DNA Translation: AAZ63104.1 Different initiation.
RefSeqiWP_041680127.1, NC_007348.1

Genome annotation databases

EnsemblBacteriaiAAZ63104; AAZ63104; Reut_B3746
KEGGireu:Reut_B3746

Similar proteinsi

Entry informationi

Entry nameiRBKTP_CUPNJ
AccessioniPrimary (citable) accession number: Q46UT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: March 28, 2018
This is version 82 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome