Reviewed, UniProtKB/Swiss-Prot Q46UA4 (HGD_RALEJ)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	Reut_B3923

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_0000225794
Sites
	Metal binding	336	1	Iron By similarity
	Metal binding	342	1	Iron By similarity
	Metal binding	372	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46UA4-1 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 293FAF36EFC3752D	FASTA	439	48,732
	10 20 30 40 50 60 MNLTHTQLAE HGYMSGFANE FATEALPGAL PVGQNSPQRA PYGLYAEQLS GTAFTAPRAH 70 80 90 100 110 120 NRRSWLYRIR PGAVHKPFTL VEQSRFLSRF DEVPPSPNQM RWSPPAMPTT PTDFIDGIVT 130 140 150 160 170 180 MAGNGGPDAM TGCGIHLYLA NQSMQDRFFY NADGEMLIVP QQGRVRFVTE MGRLDVEPQE 190 200 210 220 230 240 IVVIPRGVRF RVELPDGEAR GYICENYGAL FKLPDLGVIG SNGLANPRDF MTPVAAYEDR 250 260 270 280 290 300 EGDFELVAKF QGNLWRADIG HSPLDVVAWH GNFAPYKYDL RRFNTIGSIS FDHPDPSIFL 310 320 330 340 350 360 VLQSPSDTPG VDTIDFVIFG PRWLAMENSF RPPWFHRNIA SEFMGLITGV YDAKADGFAP 370 380 390 400 410 420 GGASLHNCMS GHGPDADTFA KATSADTSTP HHITDTMAFM FETPGVIRPT PYAARSASLQ 430 QDYYTCWQGL KKHFNPNVR

References

[1]

"Complete sequence of chromosome 2 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000091 Genomic DNA. Translation: AAZ63280.1. Different initiation.
RefSeq	YP_298124.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3614130.
GenomeReviews	Gene locus Reut_B3923 in contig CP000091_GR.
KEGG	reu:Reut_B3923.
NMPDR	fig|264198.3.peg.4480.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46UA4.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_B3923-MON.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_RALEJ

Accession

Primary (citable) accession number: Q46UA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	November 4, 2008
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)