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Q46TF2

- GLGB_CUPPJ

UniProt

Q46TF2 - GLGB_CUPPJ

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei425 – 4251NucleophileUniRule annotation
    Active sitei478 – 4781Proton donorUniRule annotation

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciCPIN264198:GIW3-4305-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
    Alpha-(1->4)-glucan branching enzymeUniRule annotation
    Glycogen branching enzymeUniRule annotation
    Short name:
    BEUniRule annotation
    Gene namesi
    Name:glgBUniRule annotation
    Ordered Locus Names:Reut_B4229
    OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
    Taxonomic identifieri264198 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000002697: Chromosome 2

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7507501,4-alpha-glucan branching enzyme GlgBPRO_0000260681Add
    BLAST

    Proteomic databases

    PRIDEiQ46TF2.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi264198.Reut_B4229.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46TF2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0296.
    HOGENOMiHOG000283037.
    KOiK00700.
    OMAiAKLLFMG.
    OrthoDBiEOG6JX7GT.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q46TF2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRVDTRPPE RRTQTISDGE LGALVEGRHR DPFAILGPHR DGDTLIVRAC    50
    VPGAHSVMLA DSRGEPLAPM TPLHAGGVFT GRLPAGVSVY QLLVRWHNGT 100
    QQVSHDPYAF GLLLGELDLH LIAEGRHFEL GACLGAQWRN VDGVQGVRFA 150
    VWAPNARRVS VIGDFNGWQP ARHPMRLRHP SGVWELFIPE AMGARPGCRY 200
    KFDLLDPHDA QLPDKADPLA LATEAPPATA SVVTQPQVSA PPFAWQDDEW 250
    MRLRNAVDPY AAPLSIYEVH VGSWLRAAND PARGWEVLAD RLIPYVHELG 300
    FTHIELLPVT EHPFGGSWGY QPLSLYAPTA RLGPPQAFAA FIDRCHRDGI 350
    GVILDWVPAH FPTDPHGLAR FDGTALYEHE DPREGFHQDW NTLIYNLGRN 400
    EVRGFLLAGA LHWLEHFHVD GLRVDAVASM LYRDYSRAPD QWVPNRFGGR 450
    ENLEAVAFLR ELNTVVHERC PGALTIAEES TAWPGVTASA ASGGLGFDFK 500
    WNMGWMHDTL RYLSLDPIHR AWHHQDMTFG TVYAWSEAFV LPLSHDEVVH 550
    GKGSMLRKCP GDDWQRFAGL RAYYGFMWAH PGKKLLFMGG ELAQWQEWNH 600
    DAELDWALLD HPMHRGVHTL VRDLNALYRE LPALHELDHS PAGFQWVVGD 650
    DHQNSVFAWL RRPAPGSGDV VLAVTNMTPV PRYGYRIGVP SEGCWHERLN 700
    TDAACYGGSN LGNGGAVTAE PVPSHGQEAS VLLTLPPLAT VILQHAGTQA 750
    Length:750
    Mass (Da):83,328
    Last modified:September 13, 2005 - v1
    Checksum:i7A95B1AAF97F5954
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000091 Genomic DNA. Translation: AAZ63582.1.
    RefSeqiYP_298426.1. NC_007348.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ63582; AAZ63582; Reut_B4229.
    GeneIDi3612659.
    KEGGireu:Reut_B4229.
    PATRICi20234299. VBIRalEut24049_4951.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000091 Genomic DNA. Translation: AAZ63582.1 .
    RefSeqi YP_298426.1. NC_007348.1.

    3D structure databases

    ProteinModelPortali Q46TF2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264198.Reut_B4229.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PRIDEi Q46TF2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ63582 ; AAZ63582 ; Reut_B4229 .
    GeneIDi 3612659.
    KEGGi reu:Reut_B4229.
    PATRICi 20234299. VBIRalEut24049_4951.

    Phylogenomic databases

    eggNOGi COG0296.
    HOGENOMi HOG000283037.
    KOi K00700.
    OMAi AKLLFMG.
    OrthoDBi EOG6JX7GT.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci CPIN264198:GIW3-4305-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 2 of Ralstonia eutropha JMP134."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JMP134 / LMG 1197.

    Entry informationi

    Entry nameiGLGB_CUPPJ
    AccessioniPrimary (citable) accession number: Q46TF2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3