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Q46TF2 (GLGB_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Reut_B4229
OrganismCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134)) [Complete proteome] [HAMAP]
Taxonomic identifier264198 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length750 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7507501,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260681

Sites

Active site4251Nucleophile By similarity
Active site4781Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46TF2 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 7A95B1AAF97F5954

FASTA75083,328
        10         20         30         40         50         60 
MNRVDTRPPE RRTQTISDGE LGALVEGRHR DPFAILGPHR DGDTLIVRAC VPGAHSVMLA 

        70         80         90        100        110        120 
DSRGEPLAPM TPLHAGGVFT GRLPAGVSVY QLLVRWHNGT QQVSHDPYAF GLLLGELDLH 

       130        140        150        160        170        180 
LIAEGRHFEL GACLGAQWRN VDGVQGVRFA VWAPNARRVS VIGDFNGWQP ARHPMRLRHP 

       190        200        210        220        230        240 
SGVWELFIPE AMGARPGCRY KFDLLDPHDA QLPDKADPLA LATEAPPATA SVVTQPQVSA 

       250        260        270        280        290        300 
PPFAWQDDEW MRLRNAVDPY AAPLSIYEVH VGSWLRAAND PARGWEVLAD RLIPYVHELG 

       310        320        330        340        350        360 
FTHIELLPVT EHPFGGSWGY QPLSLYAPTA RLGPPQAFAA FIDRCHRDGI GVILDWVPAH 

       370        380        390        400        410        420 
FPTDPHGLAR FDGTALYEHE DPREGFHQDW NTLIYNLGRN EVRGFLLAGA LHWLEHFHVD 

       430        440        450        460        470        480 
GLRVDAVASM LYRDYSRAPD QWVPNRFGGR ENLEAVAFLR ELNTVVHERC PGALTIAEES 

       490        500        510        520        530        540 
TAWPGVTASA ASGGLGFDFK WNMGWMHDTL RYLSLDPIHR AWHHQDMTFG TVYAWSEAFV 

       550        560        570        580        590        600 
LPLSHDEVVH GKGSMLRKCP GDDWQRFAGL RAYYGFMWAH PGKKLLFMGG ELAQWQEWNH 

       610        620        630        640        650        660 
DAELDWALLD HPMHRGVHTL VRDLNALYRE LPALHELDHS PAGFQWVVGD DHQNSVFAWL 

       670        680        690        700        710        720 
RRPAPGSGDV VLAVTNMTPV PRYGYRIGVP SEGCWHERLN TDAACYGGSN LGNGGAVTAE 

       730        740        750 
PVPSHGQEAS VLLTLPPLAT VILQHAGTQA 

« Hide

References

[1]"Complete sequence of chromosome 2 of Ralstonia eutropha JMP134."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000091 Genomic DNA. Translation: AAZ63582.1.
RefSeqYP_298426.1. NC_007348.1.

3D structure databases

ProteinModelPortalQ46TF2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264198.Reut_B4229.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEQ46TF2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ63582; AAZ63582; Reut_B4229.
GeneID3612659.
KEGGreu:Reut_B4229.
PATRIC20234299. VBIRalEut24049_4951.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycCPIN264198:GIW3-4305-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_CUPPJ
AccessionPrimary (citable) accession number: Q46TF2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: September 13, 2005
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries