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Q46RX3

- NAPA_CUPPJ

UniProt

Q46RX3 - NAPA_CUPPJ

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Protein

Periplasmic nitrate reductase

Gene
napA, Reut_B4763
Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi55 – 551Iron-sulfur (4Fe-4S) By similarity
Metal bindingi83 – 831Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-4839-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:Reut_B4763
OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002697: Chromosome 2

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signal Reviewed predictionAdd
BLAST
Chaini30 – 831802Periplasmic nitrate reductaseUniRule annotationPRO_0000045997Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi264198.Reut_B4763.

Structurei

3D structure databases

ProteinModelPortaliQ46RX3.
SMRiQ46RX3. Positions 41-828.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q46RX3-1 [UniParc]FASTAAdd to Basket

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MKVSRRDFIK QTAIAATASV AGIPLGTEAA NFVTDSEVTK LKWSKAPCRF    50
CGTGCGVTVA VRDNKVVATQ GDPQCEVNKG LNCVKGYFLS KIMYGQDRLT 100
KPLLRMKNGK YDKNGEFAPV TWDQAFDEME RQFKRVLKEK GPTAVGMFGS 150
GQWTVWEGYA ASKLYKAGFR SNNIDPNARH CMASAVQGFM RTFGMDEPMG 200
CYDDFEAADA FVLWGSNMAE MHPILWTRIT DRRLSHPKTR VAVLSTFTHR 250
SFDLADIPII FTPQTDLAML NYIANYIIQN NKVNKDFVNK HTVFKEGVTE 300
IGYGLRPDHP LQKAAKNAAN PGDSKPITFD DFAKFVSKYD ADYVSKLSGV 350
PKDKLRQLAE LYADPNVKVM SLWTMGFNQH TRGSWVNNMV YNVHLLTGKI 400
ATPGNSPFSL TGQPSACGTA REVGTFSHRL PADMVVTNPK HREEAERIWK 450
LPPGTIVEKP GYHAVLQNRM LRDGKLNAYW VQVNNNMQAA ANIMEEALPG 500
YRNPANFIVV SDAYPTVTAL SADLILPSAM WVEKEGAYGN AERRTQFWHQ 550
LVDAPGDARS DLWQLMEFSK RFKVEEVWPA ELVAKKPEYK GKTLFDVLYR 600
NGQVDKFPIK EVSTEYHNAE AQAFGFYVQK GLFEEYASFG RGHGHDLAPF 650
DRYHEERGLR WPVVNGKETR WRYREGSDPY VKAGTGFQFY GNPDGKAVIF 700
ALPYEPPPES PDKEYPFWLA TGRVLEHWHS GSMTRRVPEL YRAFPNAVVF 750
MHPEDAKAMG LRRGVEVEVV SRRGRMRSRV ETRGRDAPPR GLVFVPWFDA 800
SQLINKVTLD ATCPISLQTD YKKCAVKIVK V 831
Length:831
Mass (Da):93,762
Last modified:September 13, 2005 - v1
Checksum:iD0F5A7BEF139884A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000091 Genomic DNA. Translation: AAZ64111.1.
RefSeqiWP_011300877.1. NC_007348.1.
YP_298955.1. NC_007348.1.

Genome annotation databases

EnsemblBacteriaiAAZ64111; AAZ64111; Reut_B4763.
GeneIDi3613319.
KEGGireu:Reut_B4763.
PATRICi20235417. VBIRalEut24049_5510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000091 Genomic DNA. Translation: AAZ64111.1 .
RefSeqi WP_011300877.1. NC_007348.1.
YP_298955.1. NC_007348.1.

3D structure databases

ProteinModelPortali Q46RX3.
SMRi Q46RX3. Positions 41-828.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264198.Reut_B4763.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ64111 ; AAZ64111 ; Reut_B4763 .
GeneIDi 3613319.
KEGGi reu:Reut_B4763.
PATRICi 20235417. VBIRalEut24049_5510.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci CPIN264198:GIW3-4839-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 2 of Ralstonia eutropha JMP134."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JMP134 / LMG 1197.

Entry informationi

Entry nameiNAPA_CUPPJ
AccessioniPrimary (citable) accession number: Q46RX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: September 13, 2005
Last modified: September 3, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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