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Q46RX3

- NAPA_CUPPJ

UniProt

Q46RX3 - NAPA_CUPPJ

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciCPIN264198:GIW3-4839-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:Reut_B4763
    OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
    Taxonomic identifieri264198 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000002697: Chromosome 2

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
    BLAST
    Chaini30 – 831802Periplasmic nitrate reductasePRO_0000045997Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi264198.Reut_B4763.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46RX3.
    SMRiQ46RX3. Positions 41-828.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q46RX3-1 [UniParc]FASTAAdd to Basket

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    MKVSRRDFIK QTAIAATASV AGIPLGTEAA NFVTDSEVTK LKWSKAPCRF    50
    CGTGCGVTVA VRDNKVVATQ GDPQCEVNKG LNCVKGYFLS KIMYGQDRLT 100
    KPLLRMKNGK YDKNGEFAPV TWDQAFDEME RQFKRVLKEK GPTAVGMFGS 150
    GQWTVWEGYA ASKLYKAGFR SNNIDPNARH CMASAVQGFM RTFGMDEPMG 200
    CYDDFEAADA FVLWGSNMAE MHPILWTRIT DRRLSHPKTR VAVLSTFTHR 250
    SFDLADIPII FTPQTDLAML NYIANYIIQN NKVNKDFVNK HTVFKEGVTE 300
    IGYGLRPDHP LQKAAKNAAN PGDSKPITFD DFAKFVSKYD ADYVSKLSGV 350
    PKDKLRQLAE LYADPNVKVM SLWTMGFNQH TRGSWVNNMV YNVHLLTGKI 400
    ATPGNSPFSL TGQPSACGTA REVGTFSHRL PADMVVTNPK HREEAERIWK 450
    LPPGTIVEKP GYHAVLQNRM LRDGKLNAYW VQVNNNMQAA ANIMEEALPG 500
    YRNPANFIVV SDAYPTVTAL SADLILPSAM WVEKEGAYGN AERRTQFWHQ 550
    LVDAPGDARS DLWQLMEFSK RFKVEEVWPA ELVAKKPEYK GKTLFDVLYR 600
    NGQVDKFPIK EVSTEYHNAE AQAFGFYVQK GLFEEYASFG RGHGHDLAPF 650
    DRYHEERGLR WPVVNGKETR WRYREGSDPY VKAGTGFQFY GNPDGKAVIF 700
    ALPYEPPPES PDKEYPFWLA TGRVLEHWHS GSMTRRVPEL YRAFPNAVVF 750
    MHPEDAKAMG LRRGVEVEVV SRRGRMRSRV ETRGRDAPPR GLVFVPWFDA 800
    SQLINKVTLD ATCPISLQTD YKKCAVKIVK V 831
    Length:831
    Mass (Da):93,762
    Last modified:September 13, 2005 - v1
    Checksum:iD0F5A7BEF139884A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000091 Genomic DNA. Translation: AAZ64111.1.
    RefSeqiWP_011300877.1. NC_007348.1.
    YP_298955.1. NC_007348.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ64111; AAZ64111; Reut_B4763.
    GeneIDi3613319.
    KEGGireu:Reut_B4763.
    PATRICi20235417. VBIRalEut24049_5510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000091 Genomic DNA. Translation: AAZ64111.1 .
    RefSeqi WP_011300877.1. NC_007348.1.
    YP_298955.1. NC_007348.1.

    3D structure databases

    ProteinModelPortali Q46RX3.
    SMRi Q46RX3. Positions 41-828.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264198.Reut_B4763.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ64111 ; AAZ64111 ; Reut_B4763 .
    GeneIDi 3613319.
    KEGGi reu:Reut_B4763.
    PATRICi 20235417. VBIRalEut24049_5510.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci CPIN264198:GIW3-4839-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 2 of Ralstonia eutropha JMP134."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JMP134 / LMG 1197.

    Entry informationi

    Entry nameiNAPA_CUPPJ
    AccessioniPrimary (citable) accession number: Q46RX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3