Reviewed, UniProtKB/Swiss-Prot Q46RX3 (NAPA_RALEJ)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	Reut_B4763

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	831 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Tat-type signal Potential
	Chain	30 – 831	802	Periplasmic nitrate reductase HAMAP MF_01630		PRO_0000045997
Sites
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	55	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46RX3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D0F5A7BEF139884A	FASTA	831	93,762
	10 20 30 40 50 60 MKVSRRDFIK QTAIAATASV AGIPLGTEAA NFVTDSEVTK LKWSKAPCRF CGTGCGVTVA 70 80 90 100 110 120 VRDNKVVATQ GDPQCEVNKG LNCVKGYFLS KIMYGQDRLT KPLLRMKNGK YDKNGEFAPV 130 140 150 160 170 180 TWDQAFDEME RQFKRVLKEK GPTAVGMFGS GQWTVWEGYA ASKLYKAGFR SNNIDPNARH 190 200 210 220 230 240 CMASAVQGFM RTFGMDEPMG CYDDFEAADA FVLWGSNMAE MHPILWTRIT DRRLSHPKTR 250 260 270 280 290 300 VAVLSTFTHR SFDLADIPII FTPQTDLAML NYIANYIIQN NKVNKDFVNK HTVFKEGVTE 310 320 330 340 350 360 IGYGLRPDHP LQKAAKNAAN PGDSKPITFD DFAKFVSKYD ADYVSKLSGV PKDKLRQLAE 370 380 390 400 410 420 LYADPNVKVM SLWTMGFNQH TRGSWVNNMV YNVHLLTGKI ATPGNSPFSL TGQPSACGTA 430 440 450 460 470 480 REVGTFSHRL PADMVVTNPK HREEAERIWK LPPGTIVEKP GYHAVLQNRM LRDGKLNAYW 490 500 510 520 530 540 VQVNNNMQAA ANIMEEALPG YRNPANFIVV SDAYPTVTAL SADLILPSAM WVEKEGAYGN 550 560 570 580 590 600 AERRTQFWHQ LVDAPGDARS DLWQLMEFSK RFKVEEVWPA ELVAKKPEYK GKTLFDVLYR 610 620 630 640 650 660 NGQVDKFPIK EVSTEYHNAE AQAFGFYVQK GLFEEYASFG RGHGHDLAPF DRYHEERGLR 670 680 690 700 710 720 WPVVNGKETR WRYREGSDPY VKAGTGFQFY GNPDGKAVIF ALPYEPPPES PDKEYPFWLA 730 740 750 760 770 780 TGRVLEHWHS GSMTRRVPEL YRAFPNAVVF MHPEDAKAMG LRRGVEVEVV SRRGRMRSRV 790 800 810 820 830 ETRGRDAPPR GLVFVPWFDA SQLINKVTLD ATCPISLQTD YKKCAVKIVK V

References

[1]

"Complete sequence of chromosome 2 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000091 Genomic DNA. Translation: AAZ64111.1.
RefSeq	YP_298955.1.
3D structure databases
SMR	Q46RX3. Positions 41-830.
ModBase	Search...
Genome annotation databases
GeneID	3613319.
GenomeReviews	Gene locus Reut_B4763 in contig CP000091_GR.
KEGG	reu:Reut_B4763.
NMPDR	fig|264198.3.peg.5696.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46RX3.
OMA	NAYWVQV.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_B4763-MON.
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_RALEJ

Accession

Primary (citable) accession number: Q46RX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)