Reviewed, UniProtKB/Swiss-Prot Q46QQ8 (PURA2_RALEJ)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase 2
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase 2
    AdSS 2
    AMPSase 2

Gene names

Name:	purA2
Ordered Locus Names:	Reut_B5180

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Adenylosuccinate synthetase 2 HAMAP MF_00011		PRO_0000224313
Sites
	Metal binding	20	1	Magnesium By similarity
	Metal binding	47	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46QQ8-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 2FBB70A36A01F41D	FASTA	452	48,972
	10 20 30 40 50 60 MDTATKTGYA DVLVGLQYGD EGKARVVDHL AAGYDVIARF NGGANAGHTI ATSEGILRLR 70 80 90 100 110 120 QVPSGVLHPR VSLYIGSGCV IGLRQLASEI EMLAGQGIDL AGRLTISDRC PVVQPAHFLS 130 140 150 160 170 180 DRRDGGRIGT TGNGIGPCYA DLAARMRGGE RSAFQLCDLM RDESRVFEQM MRLAAQRDDE 190 200 210 220 230 240 DIAVLIQEMR QAWQVVRPFV TDNPVALLER VEGGARVLFE GAQSVMLDVV QGAQPWVTSS 250 260 270 280 290 300 HTLPSYAYVG GDLPCRYHRK TIGVAKAMVS RVGSGPLPTE LGAERSEAYC ADAGREGRGR 310 320 330 340 350 360 ADEAARFDPR ALLAQGDAFS TGIAIRMLSN EYGTGTGRPR RVGLLDVAQL QLAIRQFGVD 370 380 390 400 410 420 EVYLNKCDSL AVFAQTRDRC IPVVVGSRDG NAMHVMRFPA FDESVIPRDD ATPLPPQLET 430 440 450 LLEWLADVLG RPLRGIGLGP QRAQMRLFKT QP

References

[1]

"Complete sequence of chromosome 2 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000091 Genomic DNA. Translation: AAZ64526.1.
RefSeq	YP_299370.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3614614.
GenomeReviews	Gene locus Reut_B5180 in contig CP000091_GR.
KEGG	reu:Reut_B5180.
NMPDR	fig|264198.3.peg.6155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46QQ8.
OMA	Q46QQ8. LFEGAQS.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_B5180-MON.
Family and domain databases
HAMAP	MF_00011. Divergent sequence. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. False negative. PS00513. ADENYLOSUCCIN_SYN_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA2_RALEJ

Accession

Primary (citable) accession number: Q46QQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)