ID   3HAO_RALEJ              Reviewed;         189 AA.
AC   Q46PT7;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 28.
DE   RecName: Full=3-hydroxyanthranilate 3,4-dioxygenase;
DE            EC=1.13.11.6;
DE   AltName: Full=3-hydroxyanthranilic acid dioxygenase;
DE            Short=HAD;
DE   AltName: Full=3-hydroxyanthranilate oxygenase;
DE            Short=3-HAO;
GN   OrderedLocusNames=Reut_B5502;
OS   Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=264198;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M.,
RA   Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.;
RT   "Complete sequence of chromosome 2 of Ralstonia eutropha JMP134.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the oxidative ring opening of 3-
CC       hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde,
CC       which spontaneously cyclizes to quinolinate (By similarity).
CC   -!- CATALYTIC ACTIVITY: 3-hydroxyanthranilate + O(2) = 2-amino-3-
CC       carboxymuconate semialdehyde.
CC   -!- COFACTOR: Binds 2 Fe(2+) ions per subunit (By similarity).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the 3-HAO family.
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DR   EMBL; CP000091; AAZ64847.1; -; Genomic_DNA.
DR   RefSeq; YP_299691.1; -.
DR   SMR; Q46PT7; 4-175.
DR   GeneID; 3614547; -.
DR   GenomeReviews; CP000091_GR; Reut_B5502.
DR   KEGG; reu:Reut_B5502; -.
DR   NMPDR; fig|264198.3.peg.6202; -.
DR   HOGENOM; Q46PT7; -.
DR   OMA; Q46PT7; RHSPQRP.
DR   BioCyc; REUT264198:REUT_B5502-MON; -.
DR   GO; GO:0000334; F:3-hydroxyanthranilate 3,4-dioxygenase activity; IEA:HAMAP.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00825; -; 1.
DR   InterPro; IPR010329; 3hydroanth_dOase.
DR   Pfam; PF06052; 3-HAO; 1.
DR   TIGRFAMs; TIGR03037; anthran_nbaC; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Dioxygenase; Iron; Metal-binding; Oxidoreductase.
FT   CHAIN         1    189       3-hydroxyanthranilate 3,4-dioxygenase.
FT                                /FTId=PRO_0000245476.
FT   METAL        53     53       Iron 1; catalytic (By similarity).
FT   METAL        59     59       Iron 1; catalytic (By similarity).
FT   METAL        97     97       Iron 1; catalytic (By similarity).
FT   METAL       127    127       Iron 2 (By similarity).
FT   METAL       130    130       Iron 2 (By similarity).
FT   METAL       165    165       Iron 2 (By similarity).
FT   METAL       168    168       Iron 2 (By similarity).
FT   BINDING      49     49       Dioxygen (By similarity).
FT   BINDING      59     59       Substrate (By similarity).
FT   BINDING     101    101       Substrate (By similarity).
FT   BINDING     112    112       Substrate (By similarity).
SQ   SEQUENCE   189 AA;  21938 MW;  F3EE0A8DCEEEB74E CRC64;
     MFDLKYGRPL NFKRWLDDHA DKLKPPVGNQ QIWQDSDMMV TVVGGPNERS DFHDDPIEEL
     FYQFKGNAYL LLWEDGRYER VDLREGDMLL LPPHTLHSPQ RPEADSRCLV VERKRPQGQN
     DAFQWSCASC GTIVQRHEVT LQSIVADLPP LYEKFYASSE DARRCPGCGE IHPGRDFQAW
     HRTLARHSS
//