Reviewed, UniProtKB/Swiss-Prot Q46PT7 (3HAO_RALEJ)

Names and origin

Protein names

Recommended name:
    3-hydroxyanthranilate 3,4-dioxygenase
    EC=1.13.11.6
Alternative name(s):
    3-hydroxyanthranilic acid dioxygenase
      Short name=HAD
    3-hydroxyanthranilate oxygenase
      Short name=3-HAO

Gene names

Ordered Locus Names:

Reut_B5502

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity.
Catalytic activity	3-hydroxyanthranilate + O(2) = 2-amino-3-carboxymuconate semialdehyde. HAMAP MF_00825
Cofactor	Binds 2 Fe(2+) ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the 3-HAO family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from electronic annotation. Source: HAMAP ferrous iron binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	3-hydroxyanthranilate 3,4-dioxygenase HAMAP MF_00825		PRO_0000245476
Sites
	Metal binding	53	1	Iron 1; catalytic By similarity
	Metal binding	59	1	Iron 1; catalytic By similarity
	Metal binding	97	1	Iron 1; catalytic By similarity
	Metal binding	127	1	Iron 2 By similarity
	Metal binding	130	1	Iron 2 By similarity
	Metal binding	165	1	Iron 2 By similarity
	Metal binding	168	1	Iron 2 By similarity
	Binding site	49	1	Dioxygen By similarity
	Binding site	59	1	Substrate By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	112	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46PT7-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: F3EE0A8DCEEEB74E	FASTA	189	21,938
	10 20 30 40 50 60 MFDLKYGRPL NFKRWLDDHA DKLKPPVGNQ QIWQDSDMMV TVVGGPNERS DFHDDPIEEL 70 80 90 100 110 120 FYQFKGNAYL LLWEDGRYER VDLREGDMLL LPPHTLHSPQ RPEADSRCLV VERKRPQGQN 130 140 150 160 170 180 DAFQWSCASC GTIVQRHEVT LQSIVADLPP LYEKFYASSE DARRCPGCGE IHPGRDFQAW HRTLARHSS

References

[1]

"Complete sequence of chromosome 2 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000091 Genomic DNA. Translation: AAZ64847.1.
RefSeq	YP_299691.1.
3D structure databases
SMR	Q46PT7. Positions 4-175.
ModBase	Search...
Genome annotation databases
GeneID	3614547.
GenomeReviews	Gene locus Reut_B5502 in contig CP000091_GR.
KEGG	reu:Reut_B5502.
NMPDR	fig|264198.3.peg.6202.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46PT7.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_B5502-MON.
Family and domain databases
HAMAP	MF_00825. [Tree]
InterPro	IPR010329. 3hydroanth_dOase. [Graphical view]
Pfam	PF06052. 3-HAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR03037. anthran_nbaC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3HAO_RALEJ

Accession

Primary (citable) accession number: Q46PT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	September 13, 2005
Last modified:	November 4, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)