Q46L57 (ISPE_PROMT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	PMN2A_0279

Organism

Prochlorococcus marinus (strain NATL2A) [Complete proteome] [HAMAP]

Taxonomic identifier

59920 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235115
Regions
	Nucleotide binding	103 – 113	11	ATP Potential
Sites
	Active site	18	1	By similarity
	Active site	145	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46L57 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 2AD8F04E35C0C1C3	FASTA	319	35,688
	10 20 30 40 50 60 MVPSKANEDF LIANAHAKIN LHLEVLGIRS DGFHELAMVM QSINLSDQLK MIKRVDNTIN 70 80 90 100 110 120 LKSNNKEISN GDDNLIIKAA KLLRNKVENQ ELGVDIELEK NIPIGAGLAG GSTDAAATLL 130 140 150 160 170 180 GLNKLWKLNL KIDELENLSK EIGSDIPFCI SGGRQICFGR GEILEKLKFD QIQLGLVLVK 190 200 210 220 230 240 DPSIQVSTPV AYKKYKEQFG ESYLEDDRDF EIKRNSIRSI DWSDQSLFDN RKEIQNDLQK 250 260 270 280 290 300 SIRPMTPEVE KSLNLLSSLP DSRLVSMSGS GPSCFALFQN YDQANKVLKE HVNEFERAGL 310 SAWACSMMSN GVELRNEFT

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NATL2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000095 Genomic DNA. Translation: AAZ57771.1.
RefSeq	YP_291474.1. NC_007335.2.
3D structure databases
ProteinModelPortal	Q46L57.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46L57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3605651.
GenomeReviews	Gene locus PMN2A_0279 in contig CP000095_GR.
KEGG	pmn:PMN2A_0279.
PATRIC	23023937. VBIProMar14922_0909.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	QLCFGRG.
PhylomeDB	Q46L57.
ProtClustDB	PRK02534.
Enzyme and pathway databases
BioCyc	PMAR59920:PMN2A_0279-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_PROMT

Accession

Primary (citable) accession number: Q46L57

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program