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Q46K32

- BIOB_PROMT

UniProt

Q46K32 - BIOB_PROMT

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Protein
Biotin synthase
Gene
bioB, PMN2A_0655
Organism
Prochlorococcus marinus (strain NATL2A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi126 – 1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi158 – 1581Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi218 – 2181Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi290 – 2901Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMAR59920:GI1O-1473-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:PMN2A_0655
OrganismiProchlorococcus marinus (strain NATL2A)
Taxonomic identifieri59920 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002535: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Biotin synthaseUniRule annotation
PRO_0000381548Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi59920.PMN2A_0655.

Structurei

3D structure databases

ProteinModelPortaliQ46K32.
SMRiQ46K32. Positions 33-343.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.
PhylomeDBiQ46K32.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46K32-1 [UniParc]FASTAAdd to Basket

« Hide

MTLINPNIQE SNKLKFKDES YLDFNSIKGG DIRHDWSSEE IKEILDLPLM    50
DLLWRAQIVH RSYNPGYKVQ LASLLSVKTG GCSEDCAYCP QSVHNETTVQ 100
PNPVIEVESV LDRARAAKDA GADRFCMGWA WREIKDGNAF DSMLEMVKGV 150
RELGLEACVT AGMITDSQAS RLAEAGLTAY NHNLDTSPEH YSKIISTRTY 200
QDRLETLRRV RMAGITVCCG GIIGMGESVS DRASLLKVLA TLDPHPESVP 250
INALVAVEGT PMEDLSSIDP LEMVRMVATA RIIMPKSRIR LSAGRQQLGR 300
EAQILCLQSG ADSIFYGDTL LTTSNPEVEA DRKLLADAGI TANFS 345
Length:345
Mass (Da):37,938
Last modified:September 13, 2005 - v1
Checksum:i56DFC9866D9F87CC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000095 Genomic DNA. Translation: AAZ58146.1.
RefSeqiYP_291849.1. NC_007335.2.

Genome annotation databases

EnsemblBacteriaiAAZ58146; AAZ58146; PMN2A_0655.
GeneIDi3606032.
KEGGipmn:PMN2A_0655.
PATRICi23025035. VBIProMar14922_1453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000095 Genomic DNA. Translation: AAZ58146.1 .
RefSeqi YP_291849.1. NC_007335.2.

3D structure databases

ProteinModelPortali Q46K32.
SMRi Q46K32. Positions 33-343.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 59920.PMN2A_0655.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ58146 ; AAZ58146 ; PMN2A_0655 .
GeneIDi 3606032.
KEGGi pmn:PMN2A_0655.
PATRICi 23025035. VBIProMar14922_1453.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.
PhylomeDBi Q46K32.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PMAR59920:GI1O-1473-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NATL2A.

Entry informationi

Entry nameiBIOB_PROMT
AccessioniPrimary (citable) accession number: Q46K32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 13, 2005
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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