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Q46JG5 (DDL_PROMT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:PMN2A_0872
OrganismProchlorococcus marinus (strain NATL2A) [Complete proteome] [HAMAP]
Taxonomic identifier59920 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030484

Regions

Domain136 – 344209ATP-grasp
Nucleotide binding171 – 22656ATP By similarity

Sites

Metal binding2971Magnesium or manganese 1 By similarity
Metal binding3111Magnesium or manganese 1 By similarity
Metal binding3111Magnesium or manganese 2 By similarity
Metal binding3131Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46JG5 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 4E52A84AB0AE5D3D

FASTA34839,386
        10         20         30         40         50         60 
MFSNKQVIGV VFGGKSSEHE VSIKSAKTIY NALRYLSNKE RYIARPIYID KYGYWHDYIF 

        70         80         90        100        110        120 
SESILFDKKD HLIFEERRIN LTNLSNMEDI DVWFPCLHGP NGEDGVIQGL FKSTGKPFVG 

       130        140        150        160        170        180 
SGVLGSALGM DKIAMKSVFK SYNLPQVPYI SLNKPDIQNN LYMKSIYEQI NKIISYPCFI 

       190        200        210        220        230        240 
KPANLGSSVG ITKAYSKEEF IAGIEFAAKY DERIIVEKSI EGRELECGIL GKSIMKSSVV 

       250        260        270        280        290        300 
GEVKFQTDWY TYESKYNANL SSTIIPADLN IEISNKIQKL AIEACKAINA YGLARVDFFY 

       310        320        330        340 
QESTQQIYIN EVNTLPGFTK TSMYPTLWEA SGLKLEKLVA NLIEIAKE 

« Hide

References

[1]"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus."
Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W.
PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NATL2A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000095 Genomic DNA. Translation: AAZ58363.1.
RefSeqYP_292066.1. NC_007335.2.

3D structure databases

ProteinModelPortalQ46JG5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING59920.PMN2A_0872.

Proteomic databases

PRIDEQ46JG5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ58363; AAZ58363; PMN2A_0872.
GeneID3606255.
KEGGpmn:PMN2A_0872.
PATRIC23025535. VBIProMar14922_1697.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycPMAR59920:GI1O-1722-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PROMT
AccessionPrimary (citable) accession number: Q46JG5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways