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Q46HD5

- SYI_PROMT

UniProt

Q46HD5 - SYI_PROMT

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PMN2A_1605
Organism
Prochlorococcus marinus (strain NATL2A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei583 – 5831Aminoacyl-adenylate By similarity
Binding sitei627 – 6271ATP By similarity
Metal bindingi937 – 9371Zinc By similarity
Metal bindingi940 – 9401Zinc By similarity
Metal bindingi957 – 9571Zinc By similarity
Metal bindingi960 – 9601Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR59920:GI1O-303-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PMN2A_1605
OrganismiProchlorococcus marinus (strain NATL2A)
Taxonomic identifieri59920 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002535: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098441Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi59920.PMN2A_1605.

Structurei

3D structure databases

ProteinModelPortaliQ46HD5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionUniRule annotation
Add
BLAST
Motifi624 – 6285"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.
PhylomeDBiQ46HD5.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46HD5-1 [UniParc]FASTAAdd to Basket

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MNNIKKDSQK DRPTYKDTLN LLQTNFGMRA NATLREPELQ AFWREKNIDF    50
ELGLNNSGET FTLHDGPPYA NGTLHMGHAL NKVLKDIINK FQTMKGKKVC 100
YVPGWDCHGL PIELKVLQAM DKSQRAELTP IKLRKKAAAY AKKQVSQQMD 150
GFKRWGVWGD WDQPYLSLDK KFEASQIKLF GEMVFKGYIY RGLKPVHWSP 200
SSQTALAEAE LEYPTGHTSK SIYVGFKVNQ IPKRLTQEIS KQAPDLINSE 250
GKLKELKLVI WTTTPWTIPA NEAISVNQKL EYVIAQSSDR SLIIIANDLL 300
QEVSKSVGIN YEKRVLIKGS ILDGIIYKHP LFDKISPVVL GGDYITTESG 350
TGLVHTAPGH GVDDFNTAKK YNLSISCPVD EKGFLTKEAG KFEGLNVLKD 400
ANSVIISDLI NAGSLLKEIP YEHRYPYDWR TKKPTIFRAT EQWFASVEGF 450
RDKALSAIED VIWLPESGKN RINSMVRERG DWCISRQRTW GVPIPVFYEK 500
NGQEILLNKE TISHIADLFS VHGADIWWEY EVSELLPPSY LNQADRWQKG 550
TDTMDVWFDS GSSWSSVISK KENLNYPADL YLEGSDQHRG WFQSSLLTSV 600
AVNEHAPFKK VLTHGFALDE NGRKMSKSLG NIIDPLVIIN GGSNKKLDPA 650
YGADVLRLWV SSVDYSADVP IGSNILKQIS DVYRKVRNTS RYLLGNLYDF 700
DYKIDSIDIA NLPLLDKWML NRTAEVIDEI SDAYNNFEFS KFFQTIQNFC 750
VVDLSNFYLD IAKDRLYVSS KSDFRRRSCQ TVLSLVIEKI SGLIAPVLCH 800
MAEDIWQNIP YDLEEASVFQ RGWPNVPKSW KNSSFNCHVT ELRKLRAVIN 850
RMLESCRNNQ ALGSSLEASV RVDISDEKVK AAIEWLAESE SNNVDVLRDW 900
FLVSSLQIGG EPWAEVLVSE DNDYASVEIA KARGFKCERC WHYEIEMSKN 950
PQHTNICKRC EKVVLAI 967
Length:967
Mass (Da):110,010
Last modified:September 13, 2005 - v1
Checksum:iEDB63C23F3F932C0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000095 Genomic DNA. Translation: AAZ59093.1.
RefSeqiWP_011294238.1. NC_007335.2.
YP_292796.1. NC_007335.2.

Genome annotation databases

EnsemblBacteriaiAAZ59093; AAZ59093; PMN2A_1605.
GeneIDi3607004.
KEGGipmn:PMN2A_1605.
PATRICi23022693. VBIProMar14922_0304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000095 Genomic DNA. Translation: AAZ59093.1 .
RefSeqi WP_011294238.1. NC_007335.2.
YP_292796.1. NC_007335.2.

3D structure databases

ProteinModelPortali Q46HD5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 59920.PMN2A_1605.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ59093 ; AAZ59093 ; PMN2A_1605 .
GeneIDi 3607004.
KEGGi pmn:PMN2A_1605.
PATRICi 23022693. VBIProMar14922_0304.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.
PhylomeDBi Q46HD5.

Enzyme and pathway databases

BioCyci PMAR59920:GI1O-303-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NATL2A.

Entry informationi

Entry nameiSYI_PROMT
AccessioniPrimary (citable) accession number: Q46HD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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