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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain NATL2A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei583Aminoacyl-adenylateUniRule annotation1
Binding sitei627ATPUniRule annotation1
Metal bindingi937ZincUniRule annotation1
Metal bindingi940ZincUniRule annotation1
Metal bindingi957ZincUniRule annotation1
Metal bindingi960ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR59920:G1G4T-282-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PMN2A_1605
OrganismiProchlorococcus marinus (strain NATL2A)
Taxonomic identifieri59920 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000002535 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984411 – 967Isoleucine--tRNA ligaseAdd BLAST967

Proteomic databases

PRIDEiQ46HD5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ46HD5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi68 – 78"HIGH" regionAdd BLAST11
Motifi624 – 628"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I
PhylomeDBiQ46HD5

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q46HD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNIKKDSQK DRPTYKDTLN LLQTNFGMRA NATLREPELQ AFWREKNIDF
60 70 80 90 100
ELGLNNSGET FTLHDGPPYA NGTLHMGHAL NKVLKDIINK FQTMKGKKVC
110 120 130 140 150
YVPGWDCHGL PIELKVLQAM DKSQRAELTP IKLRKKAAAY AKKQVSQQMD
160 170 180 190 200
GFKRWGVWGD WDQPYLSLDK KFEASQIKLF GEMVFKGYIY RGLKPVHWSP
210 220 230 240 250
SSQTALAEAE LEYPTGHTSK SIYVGFKVNQ IPKRLTQEIS KQAPDLINSE
260 270 280 290 300
GKLKELKLVI WTTTPWTIPA NEAISVNQKL EYVIAQSSDR SLIIIANDLL
310 320 330 340 350
QEVSKSVGIN YEKRVLIKGS ILDGIIYKHP LFDKISPVVL GGDYITTESG
360 370 380 390 400
TGLVHTAPGH GVDDFNTAKK YNLSISCPVD EKGFLTKEAG KFEGLNVLKD
410 420 430 440 450
ANSVIISDLI NAGSLLKEIP YEHRYPYDWR TKKPTIFRAT EQWFASVEGF
460 470 480 490 500
RDKALSAIED VIWLPESGKN RINSMVRERG DWCISRQRTW GVPIPVFYEK
510 520 530 540 550
NGQEILLNKE TISHIADLFS VHGADIWWEY EVSELLPPSY LNQADRWQKG
560 570 580 590 600
TDTMDVWFDS GSSWSSVISK KENLNYPADL YLEGSDQHRG WFQSSLLTSV
610 620 630 640 650
AVNEHAPFKK VLTHGFALDE NGRKMSKSLG NIIDPLVIIN GGSNKKLDPA
660 670 680 690 700
YGADVLRLWV SSVDYSADVP IGSNILKQIS DVYRKVRNTS RYLLGNLYDF
710 720 730 740 750
DYKIDSIDIA NLPLLDKWML NRTAEVIDEI SDAYNNFEFS KFFQTIQNFC
760 770 780 790 800
VVDLSNFYLD IAKDRLYVSS KSDFRRRSCQ TVLSLVIEKI SGLIAPVLCH
810 820 830 840 850
MAEDIWQNIP YDLEEASVFQ RGWPNVPKSW KNSSFNCHVT ELRKLRAVIN
860 870 880 890 900
RMLESCRNNQ ALGSSLEASV RVDISDEKVK AAIEWLAESE SNNVDVLRDW
910 920 930 940 950
FLVSSLQIGG EPWAEVLVSE DNDYASVEIA KARGFKCERC WHYEIEMSKN
960
PQHTNICKRC EKVVLAI
Length:967
Mass (Da):110,010
Last modified:September 13, 2005 - v1
Checksum:iEDB63C23F3F932C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000095 Genomic DNA Translation: AAZ59093.1
RefSeqiWP_011294238.1, NC_007335.2

Genome annotation databases

EnsemblBacteriaiAAZ59093; AAZ59093; PMN2A_1605
KEGGipmn:PMN2A_1605

Similar proteinsi

Entry informationi

Entry nameiSYI_PROMT
AccessioniPrimary (citable) accession number: Q46HD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 13, 2005
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health