Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q46HD5

- SYI_PROMT

UniProt

Q46HD5 - SYI_PROMT

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain NATL2A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei583 – 5831Aminoacyl-adenylateUniRule annotation
    Binding sitei627 – 6271ATPUniRule annotation
    Metal bindingi937 – 9371ZincUniRule annotation
    Metal bindingi940 – 9401ZincUniRule annotation
    Metal bindingi957 – 9571ZincUniRule annotation
    Metal bindingi960 – 9601ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPMAR59920:GI1O-303-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PMN2A_1605
    OrganismiProchlorococcus marinus (strain NATL2A)
    Taxonomic identifieri59920 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
    ProteomesiUP000002535: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 967967Isoleucine--tRNA ligasePRO_0000098441Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi59920.PMN2A_1605.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46HD5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi68 – 7811"HIGH" regionAdd
    BLAST
    Motifi624 – 6285"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.
    PhylomeDBiQ46HD5.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46HD5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNIKKDSQK DRPTYKDTLN LLQTNFGMRA NATLREPELQ AFWREKNIDF    50
    ELGLNNSGET FTLHDGPPYA NGTLHMGHAL NKVLKDIINK FQTMKGKKVC 100
    YVPGWDCHGL PIELKVLQAM DKSQRAELTP IKLRKKAAAY AKKQVSQQMD 150
    GFKRWGVWGD WDQPYLSLDK KFEASQIKLF GEMVFKGYIY RGLKPVHWSP 200
    SSQTALAEAE LEYPTGHTSK SIYVGFKVNQ IPKRLTQEIS KQAPDLINSE 250
    GKLKELKLVI WTTTPWTIPA NEAISVNQKL EYVIAQSSDR SLIIIANDLL 300
    QEVSKSVGIN YEKRVLIKGS ILDGIIYKHP LFDKISPVVL GGDYITTESG 350
    TGLVHTAPGH GVDDFNTAKK YNLSISCPVD EKGFLTKEAG KFEGLNVLKD 400
    ANSVIISDLI NAGSLLKEIP YEHRYPYDWR TKKPTIFRAT EQWFASVEGF 450
    RDKALSAIED VIWLPESGKN RINSMVRERG DWCISRQRTW GVPIPVFYEK 500
    NGQEILLNKE TISHIADLFS VHGADIWWEY EVSELLPPSY LNQADRWQKG 550
    TDTMDVWFDS GSSWSSVISK KENLNYPADL YLEGSDQHRG WFQSSLLTSV 600
    AVNEHAPFKK VLTHGFALDE NGRKMSKSLG NIIDPLVIIN GGSNKKLDPA 650
    YGADVLRLWV SSVDYSADVP IGSNILKQIS DVYRKVRNTS RYLLGNLYDF 700
    DYKIDSIDIA NLPLLDKWML NRTAEVIDEI SDAYNNFEFS KFFQTIQNFC 750
    VVDLSNFYLD IAKDRLYVSS KSDFRRRSCQ TVLSLVIEKI SGLIAPVLCH 800
    MAEDIWQNIP YDLEEASVFQ RGWPNVPKSW KNSSFNCHVT ELRKLRAVIN 850
    RMLESCRNNQ ALGSSLEASV RVDISDEKVK AAIEWLAESE SNNVDVLRDW 900
    FLVSSLQIGG EPWAEVLVSE DNDYASVEIA KARGFKCERC WHYEIEMSKN 950
    PQHTNICKRC EKVVLAI 967
    Length:967
    Mass (Da):110,010
    Last modified:September 13, 2005 - v1
    Checksum:iEDB63C23F3F932C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000095 Genomic DNA. Translation: AAZ59093.1.
    RefSeqiWP_011294238.1. NC_007335.2.
    YP_292796.1. NC_007335.2.

    Genome annotation databases

    EnsemblBacteriaiAAZ59093; AAZ59093; PMN2A_1605.
    GeneIDi3607004.
    KEGGipmn:PMN2A_1605.
    PATRICi23022693. VBIProMar14922_0304.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000095 Genomic DNA. Translation: AAZ59093.1 .
    RefSeqi WP_011294238.1. NC_007335.2.
    YP_292796.1. NC_007335.2.

    3D structure databases

    ProteinModelPortali Q46HD5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 59920.PMN2A_1605.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ59093 ; AAZ59093 ; PMN2A_1605 .
    GeneIDi 3607004.
    KEGGi pmn:PMN2A_1605.
    PATRICi 23022693. VBIProMar14922_0304.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.
    PhylomeDBi Q46HD5.

    Enzyme and pathway databases

    BioCyci PMAR59920:GI1O-303-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NATL2A.

    Entry informationi

    Entry nameiSYI_PROMT
    AccessioniPrimary (citable) accession number: Q46HD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3