Q46H10 (LIPB_PROMT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Octanoyltransferase
EC=2.3.1.181

Alternative name(s):
Lipoate-protein ligase B
Lipoyl/octanoyl transferase
Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Gene names

Name:	lipB
Ordered Locus Names:	PMN2A_1736

Organism

Prochlorococcus marinus (strain NATL2A) [Complete proteome] [HAMAP]

Taxonomic identifier

59920 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Prochlorales › Prochlorococcaceae › Prochlorococcus › Prochlorococcus marinus

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate By similarity. HAMAP-Rule MF_00013
Catalytic activity	Octanoyl-[acyl-carrier-protein] + protein = protein N(6)-(octanoyl)lysine + [acyl-carrier-protein]. HAMAP-Rule MF_00013
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2. HAMAP-Rule MF_00013
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00013.
Miscellaneous	In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes By similarity. HAMAP-Rule MF_00013
Sequence similarities	Belongs to the LipB family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular protein modification process Inferred from electronic annotation. Source: InterPro lipoate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lipoyl(octanoyl) transferase activity Inferred from electronic annotation. Source: EC octanoyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Octanoyltransferase HAMAP-Rule MF_00013		PRO_0000242743
Regions
	Region	101 – 108	8	Substrate binding By similarity
	Region	168 – 170	3	Substrate binding By similarity
	Region	181 – 183	3	Substrate binding By similarity
Sites
	Active site	199	1	Acyl-thioester intermediate By similarity
	Site	165	1	Lowers pKa of active site Cys By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46H10 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: C3229908C779544C	FASTA	239	27,078
	10 20 30 40 50 60 MDKKLHFVSP ETGPNSNLDL TPQLAQSSSA FLFEPKNIVP FETALGWQKN FQKNLIEQPF 70 80 90 100 110 120 SPQAVWLLEH FSCFTMGRGS DKKNLLFEEN NSPLPVFSIE RGGEVTHHMP GQIVGYLVLN 130 140 150 160 170 180 LSLHKKDLSW YLRELEQVLI DVLDLLGMEG KRVDGLTGVW CEDKKVGSIG IGCKRWVTQH 190 200 210 220 230 GFSLNVDCDL IGFEKIIPCG LDKVKVGKLS DWIPGIKVCD VTPLLRESVK RRFKLNWEK

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NATL2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000095 Genomic DNA. Translation: AAZ59224.1.
RefSeq	YP_292927.1. NC_007335.2.
3D structure databases
ProteinModelPortal	Q46H10.
ModBase	Search...
Protein-protein interaction databases
STRING	59920.PMN2A_1736.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ59224; AAZ59224; PMN2A_1736.
GeneID	3607146.
KEGG	pmn:PMN2A_1736.
PATRIC	23022997. VBIProMar14922_0445.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0321.
HOGENOM	HOG000194322.
KO	K03801.
OMA	THHLPGQ.
ProtClustDB	PRK14344.
Enzyme and pathway databases
BioCyc	PMAR59920:GI1O-1780-MONOMER.
UniPathway	UPA00538; UER00592.
Family and domain databases
HAMAP	MF_00013. LipB.
InterPro	IPR004143. BPL_LipA_LipB. IPR000544. Octanoyltransferase. IPR020605. Octanoyltransferase_CS. [Graphical view]
PANTHER	PTHR10993:SF0. PTHR10993:SF0. 1 hit.
Pfam	PF03099. BPL_LplA_LipB. 1 hit. [Graphical view]
PIRSF	PIRSF016262. LPLase. 1 hit.
TIGRFAMs	TIGR00214. lipB. 1 hit.
PROSITE	PS01313. LIPB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPB_PROMT

Accession

Primary (citable) accession number: Q46H10

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program