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Q46GC3 (TRUB_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable tRNA pseudouridine synthase B
EC=5.4.99.25
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene names
Name:truB
Ordered Locus Names:Mbar_A0082
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina ›

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01081

Catalytic activity

tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01081

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338Probable tRNA pseudouridine synthase B HAMAP-Rule MF_01081
PRO_1000084721

Regions

Domain245 – 32076PUA

Sites

Active site781Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46GC3 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: A346867FC9192D06

FASTA33836,888
        10         20         30         40         50         60 
MSPAGKLPSE AERILVRKSG AWTNPSYGSY PEKRPILEYI EKGVVNIDKP KGPTSHEVAA 

        70         80         90        100        110        120 
WVKAILGVST AGHAGSLDPK VTGLLPTLLG KATKAVPALR LSGKEYVCLL KLHKEMPQKL 

       130        140        150        160        170        180 
VRKVCEEFTG PIYQMPPIKS AVKRVIRIRT IYYLEVLEIE GSFVLFRVGC EAGTYIRKLC 

       190        200        210        220        230        240 
HDIGLALGCG GHMQELRRTK AGPFTEETLV TLQDLKDAYV LWKEDGDESE IRRVIMPMET 

       250        260        270        280        290        300 
AVSHLPKIIL RDSAVDAICS GAALAVPGIT SLDANLKKGE LIALFTLKGE LVALAKAEMS 

       310        320        330 
TEELLKASTG LAATSVRIMM EIGTYPKGWT KKEYGVES

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000099 Genomic DNA. Translation: AAZ69069.1.
RefSeqYP_303649.1. NC_007355.1.

3D structure databases

HSSPHSSP built from PDB template 2APO based on UniProtKB Q57612.
ProteinModelPortalQ46GC3.
SMRQ46GC3. Positions 13-329.
ModBaseSearch...

Protein-protein interaction databases

STRING269797.Mbar_A0082.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ69069; AAZ69069; Mbar_A0082.
GeneID3627577.
KEGGmba:Mbar_A0082.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0130.
HOGENOMHOG000231224.
KOK11131.
OMAVDAICHG.
ProtClustDBPRK04270.

Enzyme and pathway databases

BioCycMBAR269797:GHUW-155-MONOMER.

Family and domain databases

HAMAPMF_01081. TruB_arch.
InterProIPR012960. Dyskerin-like.
IPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
[Graphical view]
PANTHERPTHR23127. PTHR23127. 1 hit.
PfamPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF55120. PsdUridine_synth_cat_dom. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR00425. CBF5. 1 hit.
PROSITEPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRUB_METBF
AccessionPrimary (citable) accession number: Q46GC3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 13, 2005
Last modified: May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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